<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with the Workiva Platform-->
<!--Copyright 2025 Workiva-->
<!--r:b595322d-a216-4bbd-8201-16491d2ef956,g:866936ac-3b56-4691-954c-c63aa0008543-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xbrldt="http://xbrl.org/2005/xbrldt" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/all" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#all"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#hypercube-dimension"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-default"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-domain"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/domain-member" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#domain-member"/>
  <link:roleRef roleURI="http://www.workiva.com/role/CoverPage" xlink:type="simple" xlink:href="wk-20241231.xsd#CoverPage"/>
  <link:definitionLink xlink:role="http://www.workiva.com/role/CoverPage" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityInformationLineItems_926eca96-07e0-4421-b5c9-14aa922f5528" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityInformationLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntitiesTable_7fec472a-cb51-42e4-b5e8-0f2206d41e4a" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntitiesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_dei_EntityInformationLineItems_926eca96-07e0-4421-b5c9-14aa922f5528" xlink:to="loc_dei_EntitiesTable_7fec472a-cb51-42e4-b5e8-0f2206d41e4a" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_8b8597cb-f182-4e7c-a623-819cf0849418" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_dei_EntitiesTable_7fec472a-cb51-42e4-b5e8-0f2206d41e4a" xlink:to="loc_us-gaap_StatementClassOfStockAxis_8b8597cb-f182-4e7c-a623-819cf0849418" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_8b8597cb-f182-4e7c-a623-819cf0849418_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_8b8597cb-f182-4e7c-a623-819cf0849418" xlink:to="loc_us-gaap_ClassOfStockDomain_8b8597cb-f182-4e7c-a623-819cf0849418_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_dbff8408-3764-49b0-8a19-26a35a08b0ef" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_8b8597cb-f182-4e7c-a623-819cf0849418" xlink:to="loc_us-gaap_ClassOfStockDomain_dbff8408-3764-49b0-8a19-26a35a08b0ef" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonClassAMember_a327613e-41d4-4d21-9059-89cabf859a0f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonClassAMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_dbff8408-3764-49b0-8a19-26a35a08b0ef" xlink:to="loc_us-gaap_CommonClassAMember_a327613e-41d4-4d21-9059-89cabf859a0f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonClassBMember_e24db33c-c83b-4ac8-8772-b979ae1dfccb" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonClassBMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_dbff8408-3764-49b0-8a19-26a35a08b0ef" xlink:to="loc_us-gaap_CommonClassBMember_e24db33c-c83b-4ac8-8772-b979ae1dfccb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentType_fad93f9f-5e50-4f4d-95b9-05825133d441" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_DocumentType"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_926eca96-07e0-4421-b5c9-14aa922f5528" xlink:to="loc_dei_DocumentType_fad93f9f-5e50-4f4d-95b9-05825133d441" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentAnnualReport_3081415d-076e-4d3c-9fc9-a94dae747378" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_DocumentAnnualReport"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_926eca96-07e0-4421-b5c9-14aa922f5528" xlink:to="loc_dei_DocumentAnnualReport_3081415d-076e-4d3c-9fc9-a94dae747378" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentPeriodEndDate_9036af31-59e7-40c3-99b4-e0b21db94c89" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_DocumentPeriodEndDate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_926eca96-07e0-4421-b5c9-14aa922f5528" xlink:to="loc_dei_DocumentPeriodEndDate_9036af31-59e7-40c3-99b4-e0b21db94c89" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_CurrentFiscalYearEndDate_2a59aa97-c774-4efe-abab-1ab9aa6e15f2" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_CurrentFiscalYearEndDate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_926eca96-07e0-4421-b5c9-14aa922f5528" xlink:to="loc_dei_CurrentFiscalYearEndDate_2a59aa97-c774-4efe-abab-1ab9aa6e15f2" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentTransitionReport_0e172ae7-4c5e-48fe-bfd9-c789fcf757ec" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_DocumentTransitionReport"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_926eca96-07e0-4421-b5c9-14aa922f5528" xlink:to="loc_dei_DocumentTransitionReport_0e172ae7-4c5e-48fe-bfd9-c789fcf757ec" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityFileNumber_f81314a3-8eee-4e02-8a45-58c6c1e56a60" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityFileNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_926eca96-07e0-4421-b5c9-14aa922f5528" xlink:to="loc_dei_EntityFileNumber_f81314a3-8eee-4e02-8a45-58c6c1e56a60" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityRegistrantName_d4279a70-6b56-402c-a53e-7590ffa64c67" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityRegistrantName"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_926eca96-07e0-4421-b5c9-14aa922f5528" xlink:to="loc_dei_EntityRegistrantName_d4279a70-6b56-402c-a53e-7590ffa64c67" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityIncorporationStateCountryCode_1f557158-692e-4811-9fcf-845a5a41848a" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityIncorporationStateCountryCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_926eca96-07e0-4421-b5c9-14aa922f5528" xlink:to="loc_dei_EntityIncorporationStateCountryCode_1f557158-692e-4811-9fcf-845a5a41848a" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityTaxIdentificationNumber_7c85956f-fceb-4183-9df6-23a0b951499c" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityTaxIdentificationNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_926eca96-07e0-4421-b5c9-14aa922f5528" xlink:to="loc_dei_EntityTaxIdentificationNumber_7c85956f-fceb-4183-9df6-23a0b951499c" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressAddressLine1_36fc7927-5e87-465e-8c84-550e35cae3d9" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityAddressAddressLine1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_926eca96-07e0-4421-b5c9-14aa922f5528" xlink:to="loc_dei_EntityAddressAddressLine1_36fc7927-5e87-465e-8c84-550e35cae3d9" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressCityOrTown_06523048-5007-4dd1-bb6d-8e97594915af" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityAddressCityOrTown"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_926eca96-07e0-4421-b5c9-14aa922f5528" xlink:to="loc_dei_EntityAddressCityOrTown_06523048-5007-4dd1-bb6d-8e97594915af" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressStateOrProvince_9af2d251-1e5e-4e74-9a13-63336d74f0ad" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityAddressStateOrProvince"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_926eca96-07e0-4421-b5c9-14aa922f5528" xlink:to="loc_dei_EntityAddressStateOrProvince_9af2d251-1e5e-4e74-9a13-63336d74f0ad" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressPostalZipCode_f8a024b4-44f3-4e4a-b076-dd9b609e03ed" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityAddressPostalZipCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_926eca96-07e0-4421-b5c9-14aa922f5528" xlink:to="loc_dei_EntityAddressPostalZipCode_f8a024b4-44f3-4e4a-b076-dd9b609e03ed" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_CityAreaCode_5866c233-f6ea-495a-8374-950403ae1aa9" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_CityAreaCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_926eca96-07e0-4421-b5c9-14aa922f5528" xlink:to="loc_dei_CityAreaCode_5866c233-f6ea-495a-8374-950403ae1aa9" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LocalPhoneNumber_eb6e65bb-a366-48f6-86ec-b5bb7bdd201f" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_LocalPhoneNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_926eca96-07e0-4421-b5c9-14aa922f5528" xlink:to="loc_dei_LocalPhoneNumber_eb6e65bb-a366-48f6-86ec-b5bb7bdd201f" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_Security12bTitle_09fce71f-bc83-432e-a85c-fbcaba53a363" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_Security12bTitle"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_926eca96-07e0-4421-b5c9-14aa922f5528" xlink:to="loc_dei_Security12bTitle_09fce71f-bc83-432e-a85c-fbcaba53a363" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_TradingSymbol_56c787aa-6758-4d37-a613-266c1e6489ba" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_TradingSymbol"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_926eca96-07e0-4421-b5c9-14aa922f5528" xlink:to="loc_dei_TradingSymbol_56c787aa-6758-4d37-a613-266c1e6489ba" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_SecurityExchangeName_146fab24-2104-45b7-a6c1-29ecf22efe5c" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_SecurityExchangeName"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_926eca96-07e0-4421-b5c9-14aa922f5528" xlink:to="loc_dei_SecurityExchangeName_146fab24-2104-45b7-a6c1-29ecf22efe5c" xlink:type="arc" order="17"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityWellKnownSeasonedIssuer_6696bfa7-ba21-4bf8-ba52-8a563f13e813" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityWellKnownSeasonedIssuer"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_926eca96-07e0-4421-b5c9-14aa922f5528" xlink:to="loc_dei_EntityWellKnownSeasonedIssuer_6696bfa7-ba21-4bf8-ba52-8a563f13e813" xlink:type="arc" order="18"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityVoluntaryFilers_dd66b9cd-1788-42b5-9e95-187ee727b906" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityVoluntaryFilers"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_926eca96-07e0-4421-b5c9-14aa922f5528" xlink:to="loc_dei_EntityVoluntaryFilers_dd66b9cd-1788-42b5-9e95-187ee727b906" xlink:type="arc" order="19"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCurrentReportingStatus_7849659d-d8e0-4d96-8478-d91a0cc0d30c" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityCurrentReportingStatus"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_926eca96-07e0-4421-b5c9-14aa922f5528" xlink:to="loc_dei_EntityCurrentReportingStatus_7849659d-d8e0-4d96-8478-d91a0cc0d30c" xlink:type="arc" order="20"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityInteractiveDataCurrent_a585248c-4a8f-4d12-aa1c-c1d8ad5e78a8" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityInteractiveDataCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_926eca96-07e0-4421-b5c9-14aa922f5528" xlink:to="loc_dei_EntityInteractiveDataCurrent_a585248c-4a8f-4d12-aa1c-c1d8ad5e78a8" xlink:type="arc" order="21"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityFilerCategory_7a288b48-3bdb-494a-a64f-75db051dfb72" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityFilerCategory"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_926eca96-07e0-4421-b5c9-14aa922f5528" xlink:to="loc_dei_EntityFilerCategory_7a288b48-3bdb-494a-a64f-75db051dfb72" xlink:type="arc" order="22"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntitySmallBusiness_7f0a3522-4312-4355-b5df-9909fa573e8b" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntitySmallBusiness"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_926eca96-07e0-4421-b5c9-14aa922f5528" xlink:to="loc_dei_EntitySmallBusiness_7f0a3522-4312-4355-b5df-9909fa573e8b" xlink:type="arc" order="23"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityEmergingGrowthCompany_bbd35223-b393-472f-ad5b-e0ea9380bbb7" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityEmergingGrowthCompany"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_926eca96-07e0-4421-b5c9-14aa922f5528" xlink:to="loc_dei_EntityEmergingGrowthCompany_bbd35223-b393-472f-ad5b-e0ea9380bbb7" xlink:type="arc" order="24"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_IcfrAuditorAttestationFlag_da6fcb42-f587-4a83-95c5-b4bdbea9cfb8" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_IcfrAuditorAttestationFlag"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_926eca96-07e0-4421-b5c9-14aa922f5528" xlink:to="loc_dei_IcfrAuditorAttestationFlag_da6fcb42-f587-4a83-95c5-b4bdbea9cfb8" xlink:type="arc" order="25"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentFinStmtErrorCorrectionFlag_5c3418da-72d8-430a-9ce1-d19610341d7c" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_DocumentFinStmtErrorCorrectionFlag"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_926eca96-07e0-4421-b5c9-14aa922f5528" xlink:to="loc_dei_DocumentFinStmtErrorCorrectionFlag_5c3418da-72d8-430a-9ce1-d19610341d7c" xlink:type="arc" order="26"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityShellCompany_58c27f06-1409-409c-817c-7efc02d44b12" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityShellCompany"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_926eca96-07e0-4421-b5c9-14aa922f5528" xlink:to="loc_dei_EntityShellCompany_58c27f06-1409-409c-817c-7efc02d44b12" xlink:type="arc" order="27"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityPublicFloat_1a038940-e34f-473a-af31-e2a4269eff9a" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityPublicFloat"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_926eca96-07e0-4421-b5c9-14aa922f5528" xlink:to="loc_dei_EntityPublicFloat_1a038940-e34f-473a-af31-e2a4269eff9a" xlink:type="arc" order="28"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCommonStockSharesOutstanding_8a5dd69d-dfc5-4be0-8acb-3ac8942319a6" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityCommonStockSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_926eca96-07e0-4421-b5c9-14aa922f5528" xlink:to="loc_dei_EntityCommonStockSharesOutstanding_8a5dd69d-dfc5-4be0-8acb-3ac8942319a6" xlink:type="arc" order="29"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentsIncorporatedByReferenceTextBlock_ae358165-36de-4174-b510-aaf00d900403" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_DocumentsIncorporatedByReferenceTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_926eca96-07e0-4421-b5c9-14aa922f5528" xlink:to="loc_dei_DocumentsIncorporatedByReferenceTextBlock_ae358165-36de-4174-b510-aaf00d900403" xlink:type="arc" order="30"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentFiscalPeriodFocus_a21ef1bc-98da-473f-97c7-614152d4db56" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_DocumentFiscalPeriodFocus"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_926eca96-07e0-4421-b5c9-14aa922f5528" xlink:to="loc_dei_DocumentFiscalPeriodFocus_a21ef1bc-98da-473f-97c7-614152d4db56" xlink:type="arc" order="31"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_AmendmentFlag_298e1f1e-59ad-480b-833e-51015f3d81ee" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_AmendmentFlag"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_926eca96-07e0-4421-b5c9-14aa922f5528" xlink:to="loc_dei_AmendmentFlag_298e1f1e-59ad-480b-833e-51015f3d81ee" xlink:type="arc" order="32"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentFiscalYearFocus_c139fe29-9e3d-4626-8d49-a8d1b7a1a21b" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_DocumentFiscalYearFocus"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_926eca96-07e0-4421-b5c9-14aa922f5528" xlink:to="loc_dei_DocumentFiscalYearFocus_c139fe29-9e3d-4626-8d49-a8d1b7a1a21b" xlink:type="arc" order="33"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCentralIndexKey_1a2df430-4c71-408e-a60c-ce51e58a0c2e" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityCentralIndexKey"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_926eca96-07e0-4421-b5c9-14aa922f5528" xlink:to="loc_dei_EntityCentralIndexKey_1a2df430-4c71-408e-a60c-ce51e58a0c2e" xlink:type="arc" order="34"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.workiva.com/role/CONSOLIDATEDBALANCESHEETS" xlink:type="simple" xlink:href="wk-20241231.xsd#CONSOLIDATEDBALANCESHEETS"/>
  <link:definitionLink xlink:role="http://www.workiva.com/role/CONSOLIDATEDBALANCESHEETS" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_ca513014-9be5-4ac0-8b27-c6e30e48f5ea" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_67cb7a40-7eb2-475b-aae8-1bbadf55b477" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_ca513014-9be5-4ac0-8b27-c6e30e48f5ea" xlink:to="loc_us-gaap_StatementTable_67cb7a40-7eb2-475b-aae8-1bbadf55b477" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_b58a333f-2600-4d2c-acbc-68a39eca5564" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_67cb7a40-7eb2-475b-aae8-1bbadf55b477" xlink:to="loc_us-gaap_StatementClassOfStockAxis_b58a333f-2600-4d2c-acbc-68a39eca5564" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_b58a333f-2600-4d2c-acbc-68a39eca5564_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_b58a333f-2600-4d2c-acbc-68a39eca5564" xlink:to="loc_us-gaap_ClassOfStockDomain_b58a333f-2600-4d2c-acbc-68a39eca5564_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_70a25208-ef8a-4f65-a8dd-154e311b6619" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_b58a333f-2600-4d2c-acbc-68a39eca5564" xlink:to="loc_us-gaap_ClassOfStockDomain_70a25208-ef8a-4f65-a8dd-154e311b6619" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonClassAMember_b4772dfe-af39-4ffe-9eee-3f18b2f05722" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonClassAMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_70a25208-ef8a-4f65-a8dd-154e311b6619" xlink:to="loc_us-gaap_CommonClassAMember_b4772dfe-af39-4ffe-9eee-3f18b2f05722" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonClassBMember_62ca48d2-6f88-48ac-b07d-b379c98f5ccc" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonClassBMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_70a25208-ef8a-4f65-a8dd-154e311b6619" xlink:to="loc_us-gaap_CommonClassBMember_62ca48d2-6f88-48ac-b07d-b379c98f5ccc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsAbstract_0bce817e-3e77-4287-8368-4c9429ccd24a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AssetsAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_ca513014-9be5-4ac0-8b27-c6e30e48f5ea" xlink:to="loc_us-gaap_AssetsAbstract_0bce817e-3e77-4287-8368-4c9429ccd24a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsCurrentAbstract_6b1a839a-8b4f-421d-8192-98908e3270f3" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AssetsCurrentAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_0bce817e-3e77-4287-8368-4c9429ccd24a" xlink:to="loc_us-gaap_AssetsCurrentAbstract_6b1a839a-8b4f-421d-8192-98908e3270f3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_0c293701-ffcf-4cfd-9a94-06cb4b3fe0e8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CashAndCashEquivalentsAtCarryingValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_6b1a839a-8b4f-421d-8192-98908e3270f3" xlink:to="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_0c293701-ffcf-4cfd-9a94-06cb4b3fe0e8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtSecuritiesCurrent_43f54754-178d-47d6-918c-d4616206bd93" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AvailableForSaleSecuritiesDebtSecuritiesCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_6b1a839a-8b4f-421d-8192-98908e3270f3" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtSecuritiesCurrent_43f54754-178d-47d6-918c-d4616206bd93" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsReceivableNetCurrent_4d68291a-b764-4aa9-b276-257f494b788e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccountsReceivableNetCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_6b1a839a-8b4f-421d-8192-98908e3270f3" xlink:to="loc_us-gaap_AccountsReceivableNetCurrent_4d68291a-b764-4aa9-b276-257f494b788e" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CapitalizedContractCostNetCurrent_764cad2e-8e36-4478-b4fe-0da72d96bbd8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CapitalizedContractCostNetCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_6b1a839a-8b4f-421d-8192-98908e3270f3" xlink:to="loc_us-gaap_CapitalizedContractCostNetCurrent_764cad2e-8e36-4478-b4fe-0da72d96bbd8" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherReceivablesNetCurrent_bcedb969-29c7-44ee-bef5-dd97a80ea6d6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherReceivablesNetCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_6b1a839a-8b4f-421d-8192-98908e3270f3" xlink:to="loc_us-gaap_OtherReceivablesNetCurrent_bcedb969-29c7-44ee-bef5-dd97a80ea6d6" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PrepaidExpenseAndOtherAssetsCurrent_5afe343d-0d45-4fcc-9325-e6049b4d553d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PrepaidExpenseAndOtherAssetsCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_6b1a839a-8b4f-421d-8192-98908e3270f3" xlink:to="loc_us-gaap_PrepaidExpenseAndOtherAssetsCurrent_5afe343d-0d45-4fcc-9325-e6049b4d553d" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsCurrent_6c698b6f-46f7-498d-9c3a-2cfc279d2b83" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AssetsCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_6b1a839a-8b4f-421d-8192-98908e3270f3" xlink:to="loc_us-gaap_AssetsCurrent_6c698b6f-46f7-498d-9c3a-2cfc279d2b83" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentAndFinanceLeaseRightOfUseAssetAfterAccumulatedDepreciationAndAmortization_9bbf1029-19a3-4cb1-ac11-6b819be16cc7" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PropertyPlantAndEquipmentAndFinanceLeaseRightOfUseAssetAfterAccumulatedDepreciationAndAmortization"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_0bce817e-3e77-4287-8368-4c9429ccd24a" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentAndFinanceLeaseRightOfUseAssetAfterAccumulatedDepreciationAndAmortization_9bbf1029-19a3-4cb1-ac11-6b819be16cc7" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseRightOfUseAsset_aef0e261-b86c-45bf-b41a-cd66c612c9b8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingLeaseRightOfUseAsset"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_0bce817e-3e77-4287-8368-4c9429ccd24a" xlink:to="loc_us-gaap_OperatingLeaseRightOfUseAsset_aef0e261-b86c-45bf-b41a-cd66c612c9b8" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CapitalizedContractCostNetNoncurrent_6d2e91cd-4c63-4329-8cad-2f54dedf3b85" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CapitalizedContractCostNetNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_0bce817e-3e77-4287-8368-4c9429ccd24a" xlink:to="loc_us-gaap_CapitalizedContractCostNetNoncurrent_6d2e91cd-4c63-4329-8cad-2f54dedf3b85" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Goodwill_1c529791-e89e-473b-ac29-8615d44a64db" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_Goodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_0bce817e-3e77-4287-8368-4c9429ccd24a" xlink:to="loc_us-gaap_Goodwill_1c529791-e89e-473b-ac29-8615d44a64db" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsNet_b2003c8a-ee98-4917-8010-c5f6f80311bc" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FiniteLivedIntangibleAssetsNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_0bce817e-3e77-4287-8368-4c9429ccd24a" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsNet_b2003c8a-ee98-4917-8010-c5f6f80311bc" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAssetsNoncurrent_f962928a-0dc9-4ff4-9158-75a0d879a53b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherAssetsNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_0bce817e-3e77-4287-8368-4c9429ccd24a" xlink:to="loc_us-gaap_OtherAssetsNoncurrent_f962928a-0dc9-4ff4-9158-75a0d879a53b" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Assets_a5086f71-67ff-47ed-a31d-5eb85056b110" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_Assets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_0bce817e-3e77-4287-8368-4c9429ccd24a" xlink:to="loc_us-gaap_Assets_a5086f71-67ff-47ed-a31d-5eb85056b110" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_032d1f47-4677-445d-9c2d-01cbf9fa1041" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LiabilitiesAndStockholdersEquityAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_ca513014-9be5-4ac0-8b27-c6e30e48f5ea" xlink:to="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_032d1f47-4677-445d-9c2d-01cbf9fa1041" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesCurrentAbstract_3dc5d1ba-cbcb-42ef-aa6f-6786ceeffd74" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LiabilitiesCurrentAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_032d1f47-4677-445d-9c2d-01cbf9fa1041" xlink:to="loc_us-gaap_LiabilitiesCurrentAbstract_3dc5d1ba-cbcb-42ef-aa6f-6786ceeffd74" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsPayableCurrent_b9884ab7-8f51-459d-966e-625d0230bb7e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccountsPayableCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesCurrentAbstract_3dc5d1ba-cbcb-42ef-aa6f-6786ceeffd74" xlink:to="loc_us-gaap_AccountsPayableCurrent_b9884ab7-8f51-459d-966e-625d0230bb7e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccruedLiabilitiesCurrent_b884dcc6-296a-4923-8010-061ea425cc5a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccruedLiabilitiesCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesCurrentAbstract_3dc5d1ba-cbcb-42ef-aa6f-6786ceeffd74" xlink:to="loc_us-gaap_AccruedLiabilitiesCurrent_b884dcc6-296a-4923-8010-061ea425cc5a" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerLiabilityCurrent_d7d6a486-9edc-46fe-b3b3-3b1b141adbea" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ContractWithCustomerLiabilityCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesCurrentAbstract_3dc5d1ba-cbcb-42ef-aa6f-6786ceeffd74" xlink:to="loc_us-gaap_ContractWithCustomerLiabilityCurrent_d7d6a486-9edc-46fe-b3b3-3b1b141adbea" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinanceLeaseLiabilityCurrent_d38edcc0-0e90-4a8c-863b-bcf28c93838f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FinanceLeaseLiabilityCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesCurrentAbstract_3dc5d1ba-cbcb-42ef-aa6f-6786ceeffd74" xlink:to="loc_us-gaap_FinanceLeaseLiabilityCurrent_d38edcc0-0e90-4a8c-863b-bcf28c93838f" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesCurrent_2ace206f-5301-4ae5-97f6-868877e2108f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LiabilitiesCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesCurrentAbstract_3dc5d1ba-cbcb-42ef-aa6f-6786ceeffd74" xlink:to="loc_us-gaap_LiabilitiesCurrent_2ace206f-5301-4ae5-97f6-868877e2108f" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConvertibleDebtNoncurrent_2544bce6-a4fe-44e4-9796-6a3296ea4943" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ConvertibleDebtNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_032d1f47-4677-445d-9c2d-01cbf9fa1041" xlink:to="loc_us-gaap_ConvertibleDebtNoncurrent_2544bce6-a4fe-44e4-9796-6a3296ea4943" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerLiabilityNoncurrent_51bd08dc-0154-49b8-83ed-47ee3e2d5579" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ContractWithCustomerLiabilityNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_032d1f47-4677-445d-9c2d-01cbf9fa1041" xlink:to="loc_us-gaap_ContractWithCustomerLiabilityNoncurrent_51bd08dc-0154-49b8-83ed-47ee3e2d5579" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherLiabilitiesNoncurrent_412ad529-6903-44cc-a0c4-ff142badccce" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherLiabilitiesNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_032d1f47-4677-445d-9c2d-01cbf9fa1041" xlink:to="loc_us-gaap_OtherLiabilitiesNoncurrent_412ad529-6903-44cc-a0c4-ff142badccce" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiabilityNoncurrent_845732f4-9b12-4b97-a6e0-b542e42b3f84" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingLeaseLiabilityNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_032d1f47-4677-445d-9c2d-01cbf9fa1041" xlink:to="loc_us-gaap_OperatingLeaseLiabilityNoncurrent_845732f4-9b12-4b97-a6e0-b542e42b3f84" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinanceLeaseLiabilityNoncurrent_ff202b22-224a-4076-9a15-a173698597d3" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FinanceLeaseLiabilityNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_032d1f47-4677-445d-9c2d-01cbf9fa1041" xlink:to="loc_us-gaap_FinanceLeaseLiabilityNoncurrent_ff202b22-224a-4076-9a15-a173698597d3" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Liabilities_c2149394-095d-43b5-a229-a230c6d78b98" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_Liabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_032d1f47-4677-445d-9c2d-01cbf9fa1041" xlink:to="loc_us-gaap_Liabilities_c2149394-095d-43b5-a229-a230c6d78b98" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquityAbstract_196303fe-7152-4ec1-b43f-ad7c1ad2b612" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockholdersEquityAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_032d1f47-4677-445d-9c2d-01cbf9fa1041" xlink:to="loc_us-gaap_StockholdersEquityAbstract_196303fe-7152-4ec1-b43f-ad7c1ad2b612" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockValue_c26b10b7-745c-4181-8fe5-92959ead5792" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonStockValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_196303fe-7152-4ec1-b43f-ad7c1ad2b612" xlink:to="loc_us-gaap_CommonStockValue_c26b10b7-745c-4181-8fe5-92959ead5792" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockValue_fdc933c5-fc85-447e-be52-7dd03cfeee00" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PreferredStockValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_196303fe-7152-4ec1-b43f-ad7c1ad2b612" xlink:to="loc_us-gaap_PreferredStockValue_fdc933c5-fc85-447e-be52-7dd03cfeee00" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapital_6aaab7e5-43d9-4881-ac21-88a36fc2b60d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AdditionalPaidInCapital"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_196303fe-7152-4ec1-b43f-ad7c1ad2b612" xlink:to="loc_us-gaap_AdditionalPaidInCapital_6aaab7e5-43d9-4881-ac21-88a36fc2b60d" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsAccumulatedDeficit_e07465d5-726d-424b-983e-edb0e7bc5352" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RetainedEarningsAccumulatedDeficit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_196303fe-7152-4ec1-b43f-ad7c1ad2b612" xlink:to="loc_us-gaap_RetainedEarningsAccumulatedDeficit_e07465d5-726d-424b-983e-edb0e7bc5352" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax_ebf3ddca-73c2-4a96-89b8-bd07e55ae9be" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_196303fe-7152-4ec1-b43f-ad7c1ad2b612" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax_ebf3ddca-73c2-4a96-89b8-bd07e55ae9be" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_7f80959d-39a6-4f68-89c0-dfabc82fe0b9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockholdersEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_196303fe-7152-4ec1-b43f-ad7c1ad2b612" xlink:to="loc_us-gaap_StockholdersEquity_7f80959d-39a6-4f68-89c0-dfabc82fe0b9" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesAndStockholdersEquity_4952d3bb-c979-4d35-b511-a6fb2513227d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LiabilitiesAndStockholdersEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_032d1f47-4677-445d-9c2d-01cbf9fa1041" xlink:to="loc_us-gaap_LiabilitiesAndStockholdersEquity_4952d3bb-c979-4d35-b511-a6fb2513227d" xlink:type="arc" order="9"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.workiva.com/role/CONSOLIDATEDBALANCESHEETSParenthetical" xlink:type="simple" xlink:href="wk-20241231.xsd#CONSOLIDATEDBALANCESHEETSParenthetical"/>
  <link:definitionLink xlink:role="http://www.workiva.com/role/CONSOLIDATEDBALANCESHEETSParenthetical" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_c22977fa-8985-4bfd-a0b3-149303d24e1e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_63058041-8bce-4bb4-8f59-642e084d12e0" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_c22977fa-8985-4bfd-a0b3-149303d24e1e" xlink:to="loc_us-gaap_StatementTable_63058041-8bce-4bb4-8f59-642e084d12e0" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_474a97d3-9783-4b98-b245-13cfd5faf198" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_63058041-8bce-4bb4-8f59-642e084d12e0" xlink:to="loc_us-gaap_StatementClassOfStockAxis_474a97d3-9783-4b98-b245-13cfd5faf198" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_474a97d3-9783-4b98-b245-13cfd5faf198_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_474a97d3-9783-4b98-b245-13cfd5faf198" xlink:to="loc_us-gaap_ClassOfStockDomain_474a97d3-9783-4b98-b245-13cfd5faf198_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_fa52c76e-e1ee-41ff-8425-1ededcae2e2b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_474a97d3-9783-4b98-b245-13cfd5faf198" xlink:to="loc_us-gaap_ClassOfStockDomain_fa52c76e-e1ee-41ff-8425-1ededcae2e2b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonClassAMember_140e8d54-9695-463e-a582-9b5939d0a08e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonClassAMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_fa52c76e-e1ee-41ff-8425-1ededcae2e2b" xlink:to="loc_us-gaap_CommonClassAMember_140e8d54-9695-463e-a582-9b5939d0a08e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonClassBMember_3140830f-11d2-4961-8b12-f62289ad909d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonClassBMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_fa52c76e-e1ee-41ff-8425-1ededcae2e2b" xlink:to="loc_us-gaap_CommonClassBMember_3140830f-11d2-4961-8b12-f62289ad909d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllowanceForDoubtfulAccountsReceivableCurrent_6d1cba55-a023-48b4-a486-012f7359b84e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AllowanceForDoubtfulAccountsReceivableCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_c22977fa-8985-4bfd-a0b3-149303d24e1e" xlink:to="loc_us-gaap_AllowanceForDoubtfulAccountsReceivableCurrent_6d1cba55-a023-48b4-a486-012f7359b84e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockParOrStatedValuePerShare_cf98136a-57f2-4577-ac95-cce4ace27a30" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonStockParOrStatedValuePerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_c22977fa-8985-4bfd-a0b3-149303d24e1e" xlink:to="loc_us-gaap_CommonStockParOrStatedValuePerShare_cf98136a-57f2-4577-ac95-cce4ace27a30" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesAuthorized_3961f986-4bef-47ff-a82d-3adfcb95d314" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonStockSharesAuthorized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_c22977fa-8985-4bfd-a0b3-149303d24e1e" xlink:to="loc_us-gaap_CommonStockSharesAuthorized_3961f986-4bef-47ff-a82d-3adfcb95d314" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesIssued_1ba35c27-9bdc-4771-8763-f124551e366c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonStockSharesIssued"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_c22977fa-8985-4bfd-a0b3-149303d24e1e" xlink:to="loc_us-gaap_CommonStockSharesIssued_1ba35c27-9bdc-4771-8763-f124551e366c" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesOutstanding_8d9b29ac-0028-40cb-92cc-26ba72d7981c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonStockSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_c22977fa-8985-4bfd-a0b3-149303d24e1e" xlink:to="loc_us-gaap_CommonStockSharesOutstanding_8d9b29ac-0028-40cb-92cc-26ba72d7981c" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockParOrStatedValuePerShare_0cd683fc-b0c5-40a8-b192-6fb160cd934b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PreferredStockParOrStatedValuePerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_c22977fa-8985-4bfd-a0b3-149303d24e1e" xlink:to="loc_us-gaap_PreferredStockParOrStatedValuePerShare_0cd683fc-b0c5-40a8-b192-6fb160cd934b" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockSharesAuthorized_ddb193ae-116d-4c67-a0f1-bd039ec3a891" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PreferredStockSharesAuthorized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_c22977fa-8985-4bfd-a0b3-149303d24e1e" xlink:to="loc_us-gaap_PreferredStockSharesAuthorized_ddb193ae-116d-4c67-a0f1-bd039ec3a891" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockSharesIssued_757b018e-e2c0-4543-82d3-ad8887124f26" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PreferredStockSharesIssued"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_c22977fa-8985-4bfd-a0b3-149303d24e1e" xlink:to="loc_us-gaap_PreferredStockSharesIssued_757b018e-e2c0-4543-82d3-ad8887124f26" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockSharesOutstanding_28cee35c-98b8-4ddc-9aaa-d44580430276" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PreferredStockSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_c22977fa-8985-4bfd-a0b3-149303d24e1e" xlink:to="loc_us-gaap_PreferredStockSharesOutstanding_28cee35c-98b8-4ddc-9aaa-d44580430276" xlink:type="arc" order="8"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.workiva.com/role/CONSOLIDATEDSTATEMENTSOFOPERATIONS" xlink:type="simple" xlink:href="wk-20241231.xsd#CONSOLIDATEDSTATEMENTSOFOPERATIONS"/>
  <link:definitionLink xlink:role="http://www.workiva.com/role/CONSOLIDATEDSTATEMENTSOFOPERATIONS" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_17521288-af03-4f14-9412-1438d488fa9b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_892425fc-d1a4-450d-a04b-3382d1477123" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_17521288-af03-4f14-9412-1438d488fa9b" xlink:to="loc_us-gaap_StatementTable_892425fc-d1a4-450d-a04b-3382d1477123" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductOrServiceAxis_704ece69-c07c-46a3-ad39-40a129e190c6" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ProductOrServiceAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_892425fc-d1a4-450d-a04b-3382d1477123" xlink:to="loc_srt_ProductOrServiceAxis_704ece69-c07c-46a3-ad39-40a129e190c6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_704ece69-c07c-46a3-ad39-40a129e190c6_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ProductOrServiceAxis_704ece69-c07c-46a3-ad39-40a129e190c6" xlink:to="loc_srt_ProductsAndServicesDomain_704ece69-c07c-46a3-ad39-40a129e190c6_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_1c7708f2-616d-40f2-aded-78faf6e9ea68" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ProductOrServiceAxis_704ece69-c07c-46a3-ad39-40a129e190c6" xlink:to="loc_srt_ProductsAndServicesDomain_1c7708f2-616d-40f2-aded-78faf6e9ea68" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LicenseAndServiceMember_ab704c15-07d8-473d-bd1d-2d22f9100de1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LicenseAndServiceMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_1c7708f2-616d-40f2-aded-78faf6e9ea68" xlink:to="loc_us-gaap_LicenseAndServiceMember_ab704c15-07d8-473d-bd1d-2d22f9100de1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_ProfessionalServicesMember_6cf0e885-410f-423f-9a70-d93948a5c186" xlink:href="wk-20241231.xsd#wk_ProfessionalServicesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_1c7708f2-616d-40f2-aded-78faf6e9ea68" xlink:to="loc_wk_ProfessionalServicesMember_6cf0e885-410f-423f-9a70-d93948a5c186" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenuesAbstract_0b9d6668-4425-41a4-b186-3ec4b5306d5b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RevenuesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_17521288-af03-4f14-9412-1438d488fa9b" xlink:to="loc_us-gaap_RevenuesAbstract_0b9d6668-4425-41a4-b186-3ec4b5306d5b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_1f16dc38-4e07-48ee-a6de-335a80aa8178" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenuesAbstract_0b9d6668-4425-41a4-b186-3ec4b5306d5b" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_1f16dc38-4e07-48ee-a6de-335a80aa8178" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostOfGoodsAndServicesSoldAbstract_f8da9a37-af95-4146-aa9e-d280b4d455dd" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CostOfGoodsAndServicesSoldAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_17521288-af03-4f14-9412-1438d488fa9b" xlink:to="loc_us-gaap_CostOfGoodsAndServicesSoldAbstract_f8da9a37-af95-4146-aa9e-d280b4d455dd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostOfGoodsAndServicesSold_5f355950-65a6-4bf1-b9ed-2a114649f2b9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CostOfGoodsAndServicesSold"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CostOfGoodsAndServicesSoldAbstract_f8da9a37-af95-4146-aa9e-d280b4d455dd" xlink:to="loc_us-gaap_CostOfGoodsAndServicesSold_5f355950-65a6-4bf1-b9ed-2a114649f2b9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GrossProfit_bc316803-3093-45f3-b1d9-9484c7596b3d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_GrossProfit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_17521288-af03-4f14-9412-1438d488fa9b" xlink:to="loc_us-gaap_GrossProfit_bc316803-3093-45f3-b1d9-9484c7596b3d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingExpensesAbstract_c0b91c38-f472-45a3-aeb5-69cbd32c1878" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingExpensesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_17521288-af03-4f14-9412-1438d488fa9b" xlink:to="loc_us-gaap_OperatingExpensesAbstract_c0b91c38-f472-45a3-aeb5-69cbd32c1878" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResearchAndDevelopmentExpense_113343a4-854a-4fc1-b693-ee7a71e25dda" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ResearchAndDevelopmentExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingExpensesAbstract_c0b91c38-f472-45a3-aeb5-69cbd32c1878" xlink:to="loc_us-gaap_ResearchAndDevelopmentExpense_113343a4-854a-4fc1-b693-ee7a71e25dda" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SellingAndMarketingExpense_5292ae9a-ae33-4ae0-82f5-dc66001a03c6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SellingAndMarketingExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingExpensesAbstract_c0b91c38-f472-45a3-aeb5-69cbd32c1878" xlink:to="loc_us-gaap_SellingAndMarketingExpense_5292ae9a-ae33-4ae0-82f5-dc66001a03c6" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GeneralAndAdministrativeExpense_99b3099c-1e45-4dc2-a83d-33e799cca808" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_GeneralAndAdministrativeExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingExpensesAbstract_c0b91c38-f472-45a3-aeb5-69cbd32c1878" xlink:to="loc_us-gaap_GeneralAndAdministrativeExpense_99b3099c-1e45-4dc2-a83d-33e799cca808" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingExpenses_72f7347e-83f1-4d63-8fce-140d4ab25538" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingExpenses"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingExpensesAbstract_c0b91c38-f472-45a3-aeb5-69cbd32c1878" xlink:to="loc_us-gaap_OperatingExpenses_72f7347e-83f1-4d63-8fce-140d4ab25538" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingIncomeLoss_5fd098a2-cf76-4f70-b630-5fe517b8d42f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_17521288-af03-4f14-9412-1438d488fa9b" xlink:to="loc_us-gaap_OperatingIncomeLoss_5fd098a2-cf76-4f70-b630-5fe517b8d42f" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InvestmentIncomeInterest_bd7e7122-075d-4a8a-bf8d-e99b44327fe4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_InvestmentIncomeInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_17521288-af03-4f14-9412-1438d488fa9b" xlink:to="loc_us-gaap_InvestmentIncomeInterest_bd7e7122-075d-4a8a-bf8d-e99b44327fe4" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestExpenseNonoperating_f037db55-325d-407e-a62b-2330bc23eb58" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_InterestExpenseNonoperating"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_17521288-af03-4f14-9412-1438d488fa9b" xlink:to="loc_us-gaap_InterestExpenseNonoperating_f037db55-325d-407e-a62b-2330bc23eb58" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherNonoperatingIncomeExpense_01c7bb58-7d60-41f7-97f3-1928f9ba8d78" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherNonoperatingIncomeExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_17521288-af03-4f14-9412-1438d488fa9b" xlink:to="loc_us-gaap_OtherNonoperatingIncomeExpense_01c7bb58-7d60-41f7-97f3-1928f9ba8d78" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_2083fd01-7c91-4adc-939a-f62f855bf9ed" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_17521288-af03-4f14-9412-1438d488fa9b" xlink:to="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_2083fd01-7c91-4adc-939a-f62f855bf9ed" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxExpenseBenefit_eb1fa035-073d-44e7-b788-23ab314700e7" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncomeTaxExpenseBenefit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_17521288-af03-4f14-9412-1438d488fa9b" xlink:to="loc_us-gaap_IncomeTaxExpenseBenefit_eb1fa035-073d-44e7-b788-23ab314700e7" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_9104e4a1-dfa3-4ef2-933d-86fba322ae11" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_17521288-af03-4f14-9412-1438d488fa9b" xlink:to="loc_us-gaap_NetIncomeLoss_9104e4a1-dfa3-4ef2-933d-86fba322ae11" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_EPSBasicAndDilutedAbstract_b8202e2f-f7e9-4898-9538-2ffe8c1ae91e" xlink:href="wk-20241231.xsd#wk_EPSBasicAndDilutedAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_17521288-af03-4f14-9412-1438d488fa9b" xlink:to="loc_wk_EPSBasicAndDilutedAbstract_b8202e2f-f7e9-4898-9538-2ffe8c1ae91e" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EarningsPerShareBasic_13960ea8-5b33-4751-98d5-04b92eb594cf" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EarningsPerShareBasic"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_wk_EPSBasicAndDilutedAbstract_b8202e2f-f7e9-4898-9538-2ffe8c1ae91e" xlink:to="loc_us-gaap_EarningsPerShareBasic_13960ea8-5b33-4751-98d5-04b92eb594cf" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EarningsPerShareDiluted_6ff6bfbd-d0f8-4730-ba5a-bd1d1e4c96a9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EarningsPerShareDiluted"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_wk_EPSBasicAndDilutedAbstract_b8202e2f-f7e9-4898-9538-2ffe8c1ae91e" xlink:to="loc_us-gaap_EarningsPerShareDiluted_6ff6bfbd-d0f8-4730-ba5a-bd1d1e4c96a9" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingBasic_2521ede5-ab74-4208-a541-dfeef98c8e25" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_WeightedAverageNumberOfSharesOutstandingBasic"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_wk_EPSBasicAndDilutedAbstract_b8202e2f-f7e9-4898-9538-2ffe8c1ae91e" xlink:to="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingBasic_2521ede5-ab74-4208-a541-dfeef98c8e25" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding_b5175bfe-e92c-47d8-a11c-43193762fcde" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_wk_EPSBasicAndDilutedAbstract_b8202e2f-f7e9-4898-9538-2ffe8c1ae91e" xlink:to="loc_us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding_b5175bfe-e92c-47d8-a11c-43193762fcde" xlink:type="arc" order="4"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.workiva.com/role/CONSOLIDATEDSTATEMENTSOFSTOCKHOLDERSEQUITYDEFICIT" xlink:type="simple" xlink:href="wk-20241231.xsd#CONSOLIDATEDSTATEMENTSOFSTOCKHOLDERSEQUITYDEFICIT"/>
  <link:definitionLink xlink:role="http://www.workiva.com/role/CONSOLIDATEDSTATEMENTSOFSTOCKHOLDERSEQUITYDEFICIT" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_2d143da9-0a00-46a2-8d4a-5dee07aee3a7" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_5e2b3848-5b80-48b3-8542-516210c069cc" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_2d143da9-0a00-46a2-8d4a-5dee07aee3a7" xlink:to="loc_us-gaap_StatementTable_5e2b3848-5b80-48b3-8542-516210c069cc" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_56f03494-52f6-4d56-846d-160a56874759" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_5e2b3848-5b80-48b3-8542-516210c069cc" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_56f03494-52f6-4d56-846d-160a56874759" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_56f03494-52f6-4d56-846d-160a56874759_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_56f03494-52f6-4d56-846d-160a56874759" xlink:to="loc_us-gaap_EquityComponentDomain_56f03494-52f6-4d56-846d-160a56874759_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_8b9cd470-7dd7-49d9-a9ab-fc6bf5fecb96" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_56f03494-52f6-4d56-846d-160a56874759" xlink:to="loc_us-gaap_EquityComponentDomain_8b9cd470-7dd7-49d9-a9ab-fc6bf5fecb96" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_bfca677e-f8cc-44b3-9741-bb15ffb78f5a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_8b9cd470-7dd7-49d9-a9ab-fc6bf5fecb96" xlink:to="loc_us-gaap_CommonStockMember_bfca677e-f8cc-44b3-9741-bb15ffb78f5a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapitalMember_f29e7eed-e7ab-4132-9f39-f6227cdd3ce6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AdditionalPaidInCapitalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_8b9cd470-7dd7-49d9-a9ab-fc6bf5fecb96" xlink:to="loc_us-gaap_AdditionalPaidInCapitalMember_f29e7eed-e7ab-4132-9f39-f6227cdd3ce6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_75cbf0ea-28b5-41be-ac1b-fdf97f414760" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_8b9cd470-7dd7-49d9-a9ab-fc6bf5fecb96" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_75cbf0ea-28b5-41be-ac1b-fdf97f414760" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsMember_854f5f94-6316-459a-aa60-3cf9dea13ec6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RetainedEarningsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_8b9cd470-7dd7-49d9-a9ab-fc6bf5fecb96" xlink:to="loc_us-gaap_RetainedEarningsMember_854f5f94-6316-459a-aa60-3cf9dea13ec6" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CumulativeEffectPeriodOfAdoptionAxis_2202b7b8-b6a8-4233-ab7c-448b362fa610" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_CumulativeEffectPeriodOfAdoptionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_5e2b3848-5b80-48b3-8542-516210c069cc" xlink:to="loc_srt_CumulativeEffectPeriodOfAdoptionAxis_2202b7b8-b6a8-4233-ab7c-448b362fa610" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CumulativeEffectPeriodOfAdoptionDomain_2202b7b8-b6a8-4233-ab7c-448b362fa610_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_CumulativeEffectPeriodOfAdoptionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_CumulativeEffectPeriodOfAdoptionAxis_2202b7b8-b6a8-4233-ab7c-448b362fa610" xlink:to="loc_srt_CumulativeEffectPeriodOfAdoptionDomain_2202b7b8-b6a8-4233-ab7c-448b362fa610_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CumulativeEffectPeriodOfAdoptionDomain_94608aeb-d7ec-443a-8399-1b4789f57cf1" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_CumulativeEffectPeriodOfAdoptionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_CumulativeEffectPeriodOfAdoptionAxis_2202b7b8-b6a8-4233-ab7c-448b362fa610" xlink:to="loc_srt_CumulativeEffectPeriodOfAdoptionDomain_94608aeb-d7ec-443a-8399-1b4789f57cf1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CumulativeEffectPeriodOfAdoptionAdjustmentMember_f8733980-4364-4854-994e-a4fc6ee96ab3" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_CumulativeEffectPeriodOfAdoptionAdjustmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_CumulativeEffectPeriodOfAdoptionDomain_94608aeb-d7ec-443a-8399-1b4789f57cf1" xlink:to="loc_srt_CumulativeEffectPeriodOfAdoptionAdjustmentMember_f8733980-4364-4854-994e-a4fc6ee96ab3" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_fe7c833d-8230-4438-aa35-3a8058897b25" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInStockholdersEquityRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_2d143da9-0a00-46a2-8d4a-5dee07aee3a7" xlink:to="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_fe7c833d-8230-4438-aa35-3a8058897b25" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharesOutstanding_5714f4ee-a1c2-4398-ac7f-790a55b81a7b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_fe7c833d-8230-4438-aa35-3a8058897b25" xlink:to="loc_us-gaap_SharesOutstanding_5714f4ee-a1c2-4398-ac7f-790a55b81a7b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_4cfc9e5e-0429-47b9-b2ea-3e51397160f7" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockholdersEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_fe7c833d-8230-4438-aa35-3a8058897b25" xlink:to="loc_us-gaap_StockholdersEquity_4cfc9e5e-0429-47b9-b2ea-3e51397160f7" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_AdditionalPaidInCapitalInducedConversionOfConvertibleSecurities_beb651e1-cd62-4584-a5f2-0f96933255cc" xlink:href="wk-20241231.xsd#wk_AdditionalPaidInCapitalInducedConversionOfConvertibleSecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_fe7c833d-8230-4438-aa35-3a8058897b25" xlink:to="loc_wk_AdditionalPaidInCapitalInducedConversionOfConvertibleSecurities_beb651e1-cd62-4584-a5f2-0f96933255cc" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue_0fbd172b-2a32-46dc-ba27-cb23d9641113" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_fe7c833d-8230-4438-aa35-3a8058897b25" xlink:to="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue_0fbd172b-2a32-46dc-ba27-cb23d9641113" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised_0b71e031-c4f2-4052-8348-9062f0bd3bc6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_fe7c833d-8230-4438-aa35-3a8058897b25" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised_0b71e031-c4f2-4052-8348-9062f0bd3bc6" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised_8aeb9cf3-e11d-46c2-9c48-b8f0d89227fa" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_fe7c833d-8230-4438-aa35-3a8058897b25" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised_8aeb9cf3-e11d-46c2-9c48-b8f0d89227fa" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesEmployeeStockPurchasePlans_17650635-3baa-41aa-8af7-602036d23619" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockIssuedDuringPeriodSharesEmployeeStockPurchasePlans"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_fe7c833d-8230-4438-aa35-3a8058897b25" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesEmployeeStockPurchasePlans_17650635-3baa-41aa-8af7-602036d23619" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueEmployeeStockPurchasePlan_85fdb918-5f47-48cf-9156-8b7b44432a17" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockIssuedDuringPeriodValueEmployeeStockPurchasePlan"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_fe7c833d-8230-4438-aa35-3a8058897b25" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueEmployeeStockPurchasePlan_85fdb918-5f47-48cf-9156-8b7b44432a17" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesRestrictedStockAwardGross_3592184c-2627-43ed-8115-f755222be1b0" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockIssuedDuringPeriodSharesRestrictedStockAwardGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_fe7c833d-8230-4438-aa35-3a8058897b25" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesRestrictedStockAwardGross_3592184c-2627-43ed-8115-f755222be1b0" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharesPaidForTaxWithholdingForShareBasedCompensation_d157d517-208e-4a54-adc5-bae68aa04d7c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SharesPaidForTaxWithholdingForShareBasedCompensation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_fe7c833d-8230-4438-aa35-3a8058897b25" xlink:to="loc_us-gaap_SharesPaidForTaxWithholdingForShareBasedCompensation_d157d517-208e-4a54-adc5-bae68aa04d7c" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdjustmentsRelatedToTaxWithholdingForShareBasedCompensation_6da9e39f-1e01-4420-8025-e51d45c5218b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AdjustmentsRelatedToTaxWithholdingForShareBasedCompensation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_fe7c833d-8230-4438-aa35-3a8058897b25" xlink:to="loc_us-gaap_AdjustmentsRelatedToTaxWithholdingForShareBasedCompensation_6da9e39f-1e01-4420-8025-e51d45c5218b" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_004b4e2f-2402-4abf-ab41-e1c7c9b2d8c3" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_fe7c833d-8230-4438-aa35-3a8058897b25" xlink:to="loc_us-gaap_NetIncomeLoss_004b4e2f-2402-4abf-ab41-e1c7c9b2d8c3" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_4dd030ad-471f-4973-9f55-16e25dd137b8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_fe7c833d-8230-4438-aa35-3a8058897b25" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_4dd030ad-471f-4973-9f55-16e25dd137b8" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharesOutstanding_d65e323c-ed53-43e0-b3d1-01c9f6f33fd1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SharesOutstanding"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_5e7feb2d-3e38-4a8f-813e-f0da29f7e0f9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockholdersEquity"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountingStandardsUpdateExtensibleList_abd04b43-f37f-428b-905a-e2e52c4a363a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccountingStandardsUpdateExtensibleList"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_2d143da9-0a00-46a2-8d4a-5dee07aee3a7" xlink:to="loc_us-gaap_AccountingStandardsUpdateExtensibleList_abd04b43-f37f-428b-905a-e2e52c4a363a" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.workiva.com/role/OrganizationandSignificantAccountingPoliciesPropertyandEquipmentnetDetails" xlink:type="simple" xlink:href="wk-20241231.xsd#OrganizationandSignificantAccountingPoliciesPropertyandEquipmentnetDetails"/>
  <link:definitionLink xlink:role="http://www.workiva.com/role/OrganizationandSignificantAccountingPoliciesPropertyandEquipmentnetDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentLineItems_7f715694-0630-46d6-beef-c83b1432cbc7" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PropertyPlantAndEquipmentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_f68dd528-0e8a-4c9d-80e2-4e8de02ed30a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfPropertyPlantAndEquipmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_7f715694-0630-46d6-beef-c83b1432cbc7" xlink:to="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_f68dd528-0e8a-4c9d-80e2-4e8de02ed30a" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_60586c57-75c6-4430-be7e-35226de2d168" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_f68dd528-0e8a-4c9d-80e2-4e8de02ed30a" xlink:to="loc_srt_RangeAxis_60586c57-75c6-4430-be7e-35226de2d168" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_60586c57-75c6-4430-be7e-35226de2d168_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_60586c57-75c6-4430-be7e-35226de2d168" xlink:to="loc_srt_RangeMember_60586c57-75c6-4430-be7e-35226de2d168_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_66a52871-59ca-4ffe-9375-a68e5d36dd82" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_60586c57-75c6-4430-be7e-35226de2d168" xlink:to="loc_srt_RangeMember_66a52871-59ca-4ffe-9375-a68e5d36dd82" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_285d3df1-34e5-4103-a72b-88e3bd3968ff" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_66a52871-59ca-4ffe-9375-a68e5d36dd82" xlink:to="loc_srt_MinimumMember_285d3df1-34e5-4103-a72b-88e3bd3968ff" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_b3762ccf-7b6c-4cd7-99d3-e2bd45354e8a" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_66a52871-59ca-4ffe-9375-a68e5d36dd82" xlink:to="loc_srt_MaximumMember_b3762ccf-7b6c-4cd7-99d3-e2bd45354e8a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentUsefulLife_2a08637a-13fb-4ff5-9ef5-ac5cb66d9e9f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PropertyPlantAndEquipmentUsefulLife"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_7f715694-0630-46d6-beef-c83b1432cbc7" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentUsefulLife_2a08637a-13fb-4ff5-9ef5-ac5cb66d9e9f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Depreciation_be8db159-20b1-47ab-b073-29568171d606" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_Depreciation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_7f715694-0630-46d6-beef-c83b1432cbc7" xlink:to="loc_us-gaap_Depreciation_be8db159-20b1-47ab-b073-29568171d606" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.workiva.com/role/OrganizationandSignificantAccountingPoliciesRevenueRecognitionandDeferredRevenueDetails" xlink:type="simple" xlink:href="wk-20241231.xsd#OrganizationandSignificantAccountingPoliciesRevenueRecognitionandDeferredRevenueDetails"/>
  <link:definitionLink xlink:role="http://www.workiva.com/role/OrganizationandSignificantAccountingPoliciesRevenueRecognitionandDeferredRevenueDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleLineItems_55e4a692-826a-4c0a-a5fc-4f63d56ca239" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleTable_228beec8-f357-4055-9527-6264e2650450" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleLineItems_55e4a692-826a-4c0a-a5fc-4f63d56ca239" xlink:to="loc_us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleTable_228beec8-f357-4055-9527-6264e2650450" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductOrServiceAxis_25d52d14-4957-4587-a8ae-6671deb02bc1" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ProductOrServiceAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleTable_228beec8-f357-4055-9527-6264e2650450" xlink:to="loc_srt_ProductOrServiceAxis_25d52d14-4957-4587-a8ae-6671deb02bc1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_25d52d14-4957-4587-a8ae-6671deb02bc1_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ProductOrServiceAxis_25d52d14-4957-4587-a8ae-6671deb02bc1" xlink:to="loc_srt_ProductsAndServicesDomain_25d52d14-4957-4587-a8ae-6671deb02bc1_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_1c97f312-e96c-409a-87e4-5889ef30d9f0" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ProductOrServiceAxis_25d52d14-4957-4587-a8ae-6671deb02bc1" xlink:to="loc_srt_ProductsAndServicesDomain_1c97f312-e96c-409a-87e4-5889ef30d9f0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LicenseAndServiceMember_3b1f22cc-7879-4c25-b8a2-2733e676d14e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LicenseAndServiceMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_1c97f312-e96c-409a-87e4-5889ef30d9f0" xlink:to="loc_us-gaap_LicenseAndServiceMember_3b1f22cc-7879-4c25-b8a2-2733e676d14e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_748e205b-37fc-4958-ac1d-43829c3dc6fc" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleTable_228beec8-f357-4055-9527-6264e2650450" xlink:to="loc_srt_RangeAxis_748e205b-37fc-4958-ac1d-43829c3dc6fc" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_748e205b-37fc-4958-ac1d-43829c3dc6fc_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_748e205b-37fc-4958-ac1d-43829c3dc6fc" xlink:to="loc_srt_RangeMember_748e205b-37fc-4958-ac1d-43829c3dc6fc_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_f7c1b2d3-92c6-4dee-a5f7-f1086b031bab" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_748e205b-37fc-4958-ac1d-43829c3dc6fc" xlink:to="loc_srt_RangeMember_f7c1b2d3-92c6-4dee-a5f7-f1086b031bab" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_ba99fae0-5274-4ad6-83e6-bc6cad4b9d97" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_f7c1b2d3-92c6-4dee-a5f7-f1086b031bab" xlink:to="loc_srt_MinimumMember_ba99fae0-5274-4ad6-83e6-bc6cad4b9d97" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_02045f28-6dc2-4a31-aa1b-05a81ef27b4e" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_f7c1b2d3-92c6-4dee-a5f7-f1086b031bab" xlink:to="loc_srt_MaximumMember_02045f28-6dc2-4a31-aa1b-05a81ef27b4e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_RevenueRecognitionCustomerContractPeriodMinimum_42d6657d-0f3e-4075-b3bd-a3747bdd0036" xlink:href="wk-20241231.xsd#wk_RevenueRecognitionCustomerContractPeriodMinimum"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleLineItems_55e4a692-826a-4c0a-a5fc-4f63d56ca239" xlink:to="loc_wk_RevenueRecognitionCustomerContractPeriodMinimum_42d6657d-0f3e-4075-b3bd-a3747bdd0036" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_RevenueRecognitionCustomerContractPeriodMaximum_f1c3e9ed-fba5-4b44-94be-1cc3a8f342a7" xlink:href="wk-20241231.xsd#wk_RevenueRecognitionCustomerContractPeriodMaximum"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleLineItems_55e4a692-826a-4c0a-a5fc-4f63d56ca239" xlink:to="loc_wk_RevenueRecognitionCustomerContractPeriodMaximum_f1c3e9ed-fba5-4b44-94be-1cc3a8f342a7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_RevenueRecognitionCustomerContractPeriodMedian_fd9e9c9e-d01b-4573-bf28-793ceaf0e03c" xlink:href="wk-20241231.xsd#wk_RevenueRecognitionCustomerContractPeriodMedian"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleLineItems_55e4a692-826a-4c0a-a5fc-4f63d56ca239" xlink:to="loc_wk_RevenueRecognitionCustomerContractPeriodMedian_fd9e9c9e-d01b-4573-bf28-793ceaf0e03c" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.workiva.com/role/OrganizationandSignificantAccountingPoliciesAccountingPronouncementsDetails" xlink:type="simple" xlink:href="wk-20241231.xsd#OrganizationandSignificantAccountingPoliciesAccountingPronouncementsDetails"/>
  <link:definitionLink xlink:role="http://www.workiva.com/role/OrganizationandSignificantAccountingPoliciesAccountingPronouncementsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleLineItems_fbbd81f3-51b5-4f35-863d-ff71a1adf6df" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleTable_cc6a3192-218c-4d5a-8aad-e8319cfba0ff" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleLineItems_fbbd81f3-51b5-4f35-863d-ff71a1adf6df" xlink:to="loc_us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleTable_cc6a3192-218c-4d5a-8aad-e8319cfba0ff" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CumulativeEffectPeriodOfAdoptionAxis_141d709b-13f0-43fd-a502-8fcb14d5d54a" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_CumulativeEffectPeriodOfAdoptionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleTable_cc6a3192-218c-4d5a-8aad-e8319cfba0ff" xlink:to="loc_srt_CumulativeEffectPeriodOfAdoptionAxis_141d709b-13f0-43fd-a502-8fcb14d5d54a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CumulativeEffectPeriodOfAdoptionDomain_141d709b-13f0-43fd-a502-8fcb14d5d54a_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_CumulativeEffectPeriodOfAdoptionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_CumulativeEffectPeriodOfAdoptionAxis_141d709b-13f0-43fd-a502-8fcb14d5d54a" xlink:to="loc_srt_CumulativeEffectPeriodOfAdoptionDomain_141d709b-13f0-43fd-a502-8fcb14d5d54a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CumulativeEffectPeriodOfAdoptionDomain_6de8ca48-1c20-4b0b-85ab-6ba249fb3cfa" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_CumulativeEffectPeriodOfAdoptionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_CumulativeEffectPeriodOfAdoptionAxis_141d709b-13f0-43fd-a502-8fcb14d5d54a" xlink:to="loc_srt_CumulativeEffectPeriodOfAdoptionDomain_6de8ca48-1c20-4b0b-85ab-6ba249fb3cfa" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CumulativeEffectPeriodOfAdoptionAdjustmentMember_42600fcd-f5b5-414b-9900-b5527cc53515" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_CumulativeEffectPeriodOfAdoptionAdjustmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_CumulativeEffectPeriodOfAdoptionDomain_6de8ca48-1c20-4b0b-85ab-6ba249fb3cfa" xlink:to="loc_srt_CumulativeEffectPeriodOfAdoptionAdjustmentMember_42600fcd-f5b5-414b-9900-b5527cc53515" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_628a40f1-ac3d-433e-ba54-dda2180c0248" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleTable_cc6a3192-218c-4d5a-8aad-e8319cfba0ff" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_628a40f1-ac3d-433e-ba54-dda2180c0248" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_628a40f1-ac3d-433e-ba54-dda2180c0248_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_628a40f1-ac3d-433e-ba54-dda2180c0248" xlink:to="loc_us-gaap_EquityComponentDomain_628a40f1-ac3d-433e-ba54-dda2180c0248_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_fe83d205-bf75-4ea4-8ec7-b09a5d65a955" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_628a40f1-ac3d-433e-ba54-dda2180c0248" xlink:to="loc_us-gaap_EquityComponentDomain_fe83d205-bf75-4ea4-8ec7-b09a5d65a955" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsMember_d94bb45e-57f6-4325-9ddb-71ec90492cc3" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RetainedEarningsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_fe83d205-bf75-4ea4-8ec7-b09a5d65a955" xlink:to="loc_us-gaap_RetainedEarningsMember_d94bb45e-57f6-4325-9ddb-71ec90492cc3" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapitalMember_ce85a13f-388e-4d6f-b328-6eadb1b84302" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AdditionalPaidInCapitalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_fe83d205-bf75-4ea4-8ec7-b09a5d65a955" xlink:to="loc_us-gaap_AdditionalPaidInCapitalMember_ce85a13f-388e-4d6f-b328-6eadb1b84302" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountingStandardsUpdateExtensibleList_a8560285-3a41-4a33-87ed-063fa2103460" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccountingStandardsUpdateExtensibleList"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleLineItems_fbbd81f3-51b5-4f35-863d-ff71a1adf6df" xlink:to="loc_us-gaap_AccountingStandardsUpdateExtensibleList_a8560285-3a41-4a33-87ed-063fa2103460" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_b65d6ca2-2b7b-4954-b8b3-2c46b7a615d9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockholdersEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleLineItems_fbbd81f3-51b5-4f35-863d-ff71a1adf6df" xlink:to="loc_us-gaap_StockholdersEquity_b65d6ca2-2b7b-4954-b8b3-2c46b7a615d9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConvertibleDebtNoncurrent_e944f00a-93a4-407f-8d22-25d2975b07e5" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ConvertibleDebtNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleLineItems_fbbd81f3-51b5-4f35-863d-ff71a1adf6df" xlink:to="loc_us-gaap_ConvertibleDebtNoncurrent_e944f00a-93a4-407f-8d22-25d2975b07e5" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.workiva.com/role/CashEquivalentsandMarketableSecuritiesScheduleofMarketableSecuritiesDetails" xlink:type="simple" xlink:href="wk-20241231.xsd#CashEquivalentsandMarketableSecuritiesScheduleofMarketableSecuritiesDetails"/>
  <link:definitionLink xlink:role="http://www.workiva.com/role/CashEquivalentsandMarketableSecuritiesScheduleofMarketableSecuritiesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsLineItems_c022d3c8-08fd-45fd-92aa-18e942ae2897" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CashAndCashEquivalentsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfCashAndCashEquivalentsTable_38d35fda-443a-417f-be43-a39075e3da8a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfCashAndCashEquivalentsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_CashAndCashEquivalentsLineItems_c022d3c8-08fd-45fd-92aa-18e942ae2897" xlink:to="loc_us-gaap_ScheduleOfCashAndCashEquivalentsTable_38d35fda-443a-417f-be43-a39075e3da8a" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsAxis_cef0d774-e2db-415b-87dd-02fadd2fcfdf" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CashAndCashEquivalentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfCashAndCashEquivalentsTable_38d35fda-443a-417f-be43-a39075e3da8a" xlink:to="loc_us-gaap_CashAndCashEquivalentsAxis_cef0d774-e2db-415b-87dd-02fadd2fcfdf" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_cef0d774-e2db-415b-87dd-02fadd2fcfdf_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_CashAndCashEquivalentsAxis_cef0d774-e2db-415b-87dd-02fadd2fcfdf" xlink:to="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_cef0d774-e2db-415b-87dd-02fadd2fcfdf_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_ada911ab-f54d-458b-9090-2663119cd20e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_CashAndCashEquivalentsAxis_cef0d774-e2db-415b-87dd-02fadd2fcfdf" xlink:to="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_ada911ab-f54d-458b-9090-2663119cd20e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MoneyMarketFundsMember_3f29ccd5-5349-4fec-969d-7216c1b7bfba" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_MoneyMarketFundsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_ada911ab-f54d-458b-9090-2663119cd20e" xlink:to="loc_us-gaap_MoneyMarketFundsMember_3f29ccd5-5349-4fec-969d-7216c1b7bfba" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashEquivalentsMember_e2958572-9dc2-4971-a630-b369c29648d0" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CashEquivalentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_ada911ab-f54d-458b-9090-2663119cd20e" xlink:to="loc_us-gaap_CashEquivalentsMember_e2958572-9dc2-4971-a630-b369c29648d0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_f502ce0d-5c86-4ad2-9c7d-7f8857badd4f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CashAndCashEquivalentsAtCarryingValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CashAndCashEquivalentsLineItems_c022d3c8-08fd-45fd-92aa-18e942ae2897" xlink:to="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_f502ce0d-5c86-4ad2-9c7d-7f8857badd4f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_CashAndCashEquivalentsAccumulatedGrossUnrealizedGainBeforeTax_4196246e-de80-4904-9f1e-d4edc352433d" xlink:href="wk-20241231.xsd#wk_CashAndCashEquivalentsAccumulatedGrossUnrealizedGainBeforeTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CashAndCashEquivalentsLineItems_c022d3c8-08fd-45fd-92aa-18e942ae2897" xlink:to="loc_wk_CashAndCashEquivalentsAccumulatedGrossUnrealizedGainBeforeTax_4196246e-de80-4904-9f1e-d4edc352433d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsFairValueDisclosure_c9e64f6c-3743-4556-8d9f-14c35622c42b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CashAndCashEquivalentsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CashAndCashEquivalentsLineItems_c022d3c8-08fd-45fd-92aa-18e942ae2897" xlink:to="loc_us-gaap_CashAndCashEquivalentsFairValueDisclosure_c9e64f6c-3743-4556-8d9f-14c35622c42b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_ca967496-d361-4b01-88f1-758bf6bf3c84" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_5c786878-495b-4af1-8b93-69d373f790f1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtSecuritiesAvailableForSaleTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_ca967496-d361-4b01-88f1-758bf6bf3c84" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_5c786878-495b-4af1-8b93-69d373f790f1" xlink:type="arc" order="2" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentAxis_84e8262f-71eb-4acf-b2fc-66eaecec83ef" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FinancialInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_5c786878-495b-4af1-8b93-69d373f790f1" xlink:to="loc_us-gaap_FinancialInstrumentAxis_84e8262f-71eb-4acf-b2fc-66eaecec83ef" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_84e8262f-71eb-4acf-b2fc-66eaecec83ef_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancialInstrumentAxis_84e8262f-71eb-4acf-b2fc-66eaecec83ef" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_84e8262f-71eb-4acf-b2fc-66eaecec83ef_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_a477922a-4318-4884-87f5-7406c9c83ee9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancialInstrumentAxis_84e8262f-71eb-4acf-b2fc-66eaecec83ef" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_a477922a-4318-4884-87f5-7406c9c83ee9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommercialPaperMember_b0be3edb-1f5c-4a48-9153-c5b0e1965e44" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommercialPaperMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_a477922a-4318-4884-87f5-7406c9c83ee9" xlink:to="loc_us-gaap_CommercialPaperMember_b0be3edb-1f5c-4a48-9153-c5b0e1965e44" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USTreasurySecuritiesMember_9fb0c1eb-f7d2-4c62-b5be-d50fad52fcbf" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_USTreasurySecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_a477922a-4318-4884-87f5-7406c9c83ee9" xlink:to="loc_us-gaap_USTreasurySecuritiesMember_9fb0c1eb-f7d2-4c62-b5be-d50fad52fcbf" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USGovernmentAgenciesDebtSecuritiesMember_270674c2-8aec-429a-8e19-f83fdd5dcb82" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_USGovernmentAgenciesDebtSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_a477922a-4318-4884-87f5-7406c9c83ee9" xlink:to="loc_us-gaap_USGovernmentAgenciesDebtSecuritiesMember_270674c2-8aec-429a-8e19-f83fdd5dcb82" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CorporateDebtSecuritiesMember_f0b4a8ef-ca94-4eb7-b59a-fa9d4ab4e9dc" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CorporateDebtSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_a477922a-4318-4884-87f5-7406c9c83ee9" xlink:to="loc_us-gaap_CorporateDebtSecuritiesMember_f0b4a8ef-ca94-4eb7-b59a-fa9d4ab4e9dc" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ForeignGovernmentDebtSecuritiesMember_fe4dd400-5f17-42e6-80c5-676ed361443f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ForeignGovernmentDebtSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_a477922a-4318-4884-87f5-7406c9c83ee9" xlink:to="loc_us-gaap_ForeignGovernmentDebtSecuritiesMember_fe4dd400-5f17-42e6-80c5-676ed361443f" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleDebtSecuritiesAmortizedCostBasis_14c7123c-48b2-46e4-bb55-5079736a9d19" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AvailableForSaleDebtSecuritiesAmortizedCostBasis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_ca967496-d361-4b01-88f1-758bf6bf3c84" xlink:to="loc_us-gaap_AvailableForSaleDebtSecuritiesAmortizedCostBasis_14c7123c-48b2-46e4-bb55-5079736a9d19" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedGainBeforeTax_49f8ffdf-f613-40e6-85ec-370d96f388be" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedGainBeforeTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_ca967496-d361-4b01-88f1-758bf6bf3c84" xlink:to="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedGainBeforeTax_49f8ffdf-f613-40e6-85ec-370d96f388be" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedLossBeforeTax_66946394-9e7c-4182-aba5-bbc0a6e5a0b5" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedLossBeforeTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_ca967496-d361-4b01-88f1-758bf6bf3c84" xlink:to="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedLossBeforeTax_66946394-9e7c-4182-aba5-bbc0a6e5a0b5" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_112b2d98-2379-45a6-9e7c-24239f7a9471" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AvailableForSaleSecuritiesDebtSecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_ca967496-d361-4b01-88f1-758bf6bf3c84" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_112b2d98-2379-45a6-9e7c-24239f7a9471" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_CashCashEquivalentsAndAvailableForSaleDebtSecuritiesAmortizedCost_2dcb0945-d122-413f-8e35-66d7945212b8" xlink:href="wk-20241231.xsd#wk_CashCashEquivalentsAndAvailableForSaleDebtSecuritiesAmortizedCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_ca967496-d361-4b01-88f1-758bf6bf3c84" xlink:to="loc_wk_CashCashEquivalentsAndAvailableForSaleDebtSecuritiesAmortizedCost_2dcb0945-d122-413f-8e35-66d7945212b8" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_CashAndCashEquivalentsAndDebtSecuritiesAvailableForSaleAccumulatedGrossUnrealizedGainBeforeTax_7806f1a1-0162-463a-883e-707b5e4d537f" xlink:href="wk-20241231.xsd#wk_CashAndCashEquivalentsAndDebtSecuritiesAvailableForSaleAccumulatedGrossUnrealizedGainBeforeTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_ca967496-d361-4b01-88f1-758bf6bf3c84" xlink:to="loc_wk_CashAndCashEquivalentsAndDebtSecuritiesAvailableForSaleAccumulatedGrossUnrealizedGainBeforeTax_7806f1a1-0162-463a-883e-707b5e4d537f" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_CashCashEquivalentsAndAvailableForSaleDebtSecurities_19625a51-c03e-45a2-a921-cccaa8b534c6" xlink:href="wk-20241231.xsd#wk_CashCashEquivalentsAndAvailableForSaleDebtSecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_ca967496-d361-4b01-88f1-758bf6bf3c84" xlink:to="loc_wk_CashCashEquivalentsAndAvailableForSaleDebtSecurities_19625a51-c03e-45a2-a921-cccaa8b534c6" xlink:type="arc" order="6"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.workiva.com/role/CashEquivalentsandMarketableSecuritiesContinuousUnrealizedLossPositionDetails" xlink:type="simple" xlink:href="wk-20241231.xsd#CashEquivalentsandMarketableSecuritiesContinuousUnrealizedLossPositionDetails"/>
  <link:definitionLink xlink:role="http://www.workiva.com/role/CashEquivalentsandMarketableSecuritiesContinuousUnrealizedLossPositionDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesContinuousUnrealizedLossPositionFairValueAbstract_4a63565a-80cc-425c-b0db-f21611b87052" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AvailableForSaleSecuritiesContinuousUnrealizedLossPositionFairValueAbstract"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleUnrealizedLossPositionFairValueTable_2c4bf5ce-fc6f-4c81-b4bb-3767aabfcb7a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtSecuritiesAvailableForSaleUnrealizedLossPositionFairValueTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesContinuousUnrealizedLossPositionFairValueAbstract_4a63565a-80cc-425c-b0db-f21611b87052" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleUnrealizedLossPositionFairValueTable_2c4bf5ce-fc6f-4c81-b4bb-3767aabfcb7a" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentAxis_e2b0d300-7638-4651-9196-e6fea1ea3363" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FinancialInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtSecuritiesAvailableForSaleUnrealizedLossPositionFairValueTable_2c4bf5ce-fc6f-4c81-b4bb-3767aabfcb7a" xlink:to="loc_us-gaap_FinancialInstrumentAxis_e2b0d300-7638-4651-9196-e6fea1ea3363" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_e2b0d300-7638-4651-9196-e6fea1ea3363_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancialInstrumentAxis_e2b0d300-7638-4651-9196-e6fea1ea3363" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_e2b0d300-7638-4651-9196-e6fea1ea3363_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_fe8b68a9-65b1-4601-acad-b99067c9b5fb" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancialInstrumentAxis_e2b0d300-7638-4651-9196-e6fea1ea3363" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_fe8b68a9-65b1-4601-acad-b99067c9b5fb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USTreasurySecuritiesMember_9e9b1425-8aa5-426a-83cb-dcad45340249" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_USTreasurySecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_fe8b68a9-65b1-4601-acad-b99067c9b5fb" xlink:to="loc_us-gaap_USTreasurySecuritiesMember_9e9b1425-8aa5-426a-83cb-dcad45340249" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USGovernmentAgenciesDebtSecuritiesMember_e251b8dd-738d-4c21-8ac9-64e29eef89fb" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_USGovernmentAgenciesDebtSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_fe8b68a9-65b1-4601-acad-b99067c9b5fb" xlink:to="loc_us-gaap_USGovernmentAgenciesDebtSecuritiesMember_e251b8dd-738d-4c21-8ac9-64e29eef89fb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CorporateDebtSecuritiesMember_b005230b-9544-4672-a8c8-6d3595488bdf" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CorporateDebtSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_fe8b68a9-65b1-4601-acad-b99067c9b5fb" xlink:to="loc_us-gaap_CorporateDebtSecuritiesMember_b005230b-9544-4672-a8c8-6d3595488bdf" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableforSaleUnrealizedLossPositionAbstract_e6b8225b-571b-487e-96e8-9f86f245bb64" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtSecuritiesAvailableforSaleUnrealizedLossPositionAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesContinuousUnrealizedLossPositionFairValueAbstract_4a63565a-80cc-425c-b0db-f21611b87052" xlink:to="loc_us-gaap_DebtSecuritiesAvailableforSaleUnrealizedLossPositionAbstract_e6b8225b-571b-487e-96e8-9f86f245bb64" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPositionLessThan12Months_9f9e64d7-acd3-4841-b4c3-fdf0d69af5eb" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPositionLessThan12Months"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtSecuritiesAvailableforSaleUnrealizedLossPositionAbstract_e6b8225b-571b-487e-96e8-9f86f245bb64" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPositionLessThan12Months_9f9e64d7-acd3-4841-b4c3-fdf0d69af5eb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPosition12MonthsOrLonger_58e44e9e-0de6-4c74-ad9b-a98672399292" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPosition12MonthsOrLonger"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtSecuritiesAvailableforSaleUnrealizedLossPositionAbstract_e6b8225b-571b-487e-96e8-9f86f245bb64" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPosition12MonthsOrLonger_58e44e9e-0de6-4c74-ad9b-a98672399292" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesContinuousUnrealizedLossPositionAccumulatedLossAbstract_a74cb062-8770-4171-b7ea-f6afd12af4e6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AvailableForSaleSecuritiesContinuousUnrealizedLossPositionAccumulatedLossAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesContinuousUnrealizedLossPositionFairValueAbstract_4a63565a-80cc-425c-b0db-f21611b87052" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesContinuousUnrealizedLossPositionAccumulatedLossAbstract_a74cb062-8770-4171-b7ea-f6afd12af4e6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPositionLessThan12MonthsAccumulatedLoss_1340d364-ed23-4383-ab64-fe1e80090e13" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPositionLessThan12MonthsAccumulatedLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesContinuousUnrealizedLossPositionAccumulatedLossAbstract_a74cb062-8770-4171-b7ea-f6afd12af4e6" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPositionLessThan12MonthsAccumulatedLoss_1340d364-ed23-4383-ab64-fe1e80090e13" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPosition12MonthsOrLongerAccumulatedLoss_fc11c883-854a-431a-b88e-b5b71f10f408" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPosition12MonthsOrLongerAccumulatedLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesContinuousUnrealizedLossPositionAccumulatedLossAbstract_a74cb062-8770-4171-b7ea-f6afd12af4e6" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPosition12MonthsOrLongerAccumulatedLoss_fc11c883-854a-431a-b88e-b5b71f10f408" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.workiva.com/role/SupplementalConsolidatedBalanceSheetInformationPropertyandEquipmentDetails" xlink:type="simple" xlink:href="wk-20241231.xsd#SupplementalConsolidatedBalanceSheetInformationPropertyandEquipmentDetails"/>
  <link:definitionLink xlink:role="http://www.workiva.com/role/SupplementalConsolidatedBalanceSheetInformationPropertyandEquipmentDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentLineItems_5131c8ee-1ed7-49a2-a482-cca1bffbd73e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PropertyPlantAndEquipmentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_d4e2899f-67c6-4327-b5eb-c701822f1d55" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfPropertyPlantAndEquipmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_5131c8ee-1ed7-49a2-a482-cca1bffbd73e" xlink:to="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_d4e2899f-67c6-4327-b5eb-c701822f1d55" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_3a8029c9-a4f0-43c1-94a9-58113a1721c2" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PropertyPlantAndEquipmentByTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_d4e2899f-67c6-4327-b5eb-c701822f1d55" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_3a8029c9-a4f0-43c1-94a9-58113a1721c2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_3a8029c9-a4f0-43c1-94a9-58113a1721c2_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_3a8029c9-a4f0-43c1-94a9-58113a1721c2" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_3a8029c9-a4f0-43c1-94a9-58113a1721c2_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_d713f383-6a0e-4a9f-adcf-6a6fa8210f33" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_3a8029c9-a4f0-43c1-94a9-58113a1721c2" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_d713f383-6a0e-4a9f-adcf-6a6fa8210f33" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_ComputersEquipmentAndSoftwareMember_18752b9a-73e6-4684-a0c7-7730cded24a1" xlink:href="wk-20241231.xsd#wk_ComputersEquipmentAndSoftwareMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_d713f383-6a0e-4a9f-adcf-6a6fa8210f33" xlink:to="loc_wk_ComputersEquipmentAndSoftwareMember_18752b9a-73e6-4684-a0c7-7730cded24a1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FurnitureAndFixturesMember_c3e2d5ee-6512-4905-9c82-1248f8d3cbd2" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FurnitureAndFixturesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_d713f383-6a0e-4a9f-adcf-6a6fa8210f33" xlink:to="loc_us-gaap_FurnitureAndFixturesMember_c3e2d5ee-6512-4905-9c82-1248f8d3cbd2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VehiclesMember_ba6d8709-79b5-4909-96d1-daaf1969fc91" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_VehiclesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_d713f383-6a0e-4a9f-adcf-6a6fa8210f33" xlink:to="loc_us-gaap_VehiclesMember_ba6d8709-79b5-4909-96d1-daaf1969fc91" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LeaseholdImprovementsMember_14d7020d-fbf0-4642-94cf-aa4cce4b87fc" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LeaseholdImprovementsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_d713f383-6a0e-4a9f-adcf-6a6fa8210f33" xlink:to="loc_us-gaap_LeaseholdImprovementsMember_14d7020d-fbf0-4642-94cf-aa4cce4b87fc" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConstructionInProgressMember_96bae395-4e52-4a5b-a25d-d77a18b55b4e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ConstructionInProgressMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_d713f383-6a0e-4a9f-adcf-6a6fa8210f33" xlink:to="loc_us-gaap_ConstructionInProgressMember_96bae395-4e52-4a5b-a25d-d77a18b55b4e" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinanceLeaseRightOfUseAssetBeforeAccumulatedAmortization_df532272-ea54-4a81-8c55-031ee78843db" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FinanceLeaseRightOfUseAssetBeforeAccumulatedAmortization"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_5131c8ee-1ed7-49a2-a482-cca1bffbd73e" xlink:to="loc_us-gaap_FinanceLeaseRightOfUseAssetBeforeAccumulatedAmortization_df532272-ea54-4a81-8c55-031ee78843db" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentGross_7e5d24bb-5287-41c1-8b99-7c95808e7f77" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PropertyPlantAndEquipmentGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_5131c8ee-1ed7-49a2-a482-cca1bffbd73e" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentGross_7e5d24bb-5287-41c1-8b99-7c95808e7f77" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentAndFinanceLeaseRightOfUseAssetBeforeAccumulatedDepreciationAndAmortization_99c6bd4f-102d-4e60-8757-b2a77b7a622f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PropertyPlantAndEquipmentAndFinanceLeaseRightOfUseAssetBeforeAccumulatedDepreciationAndAmortization"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_5131c8ee-1ed7-49a2-a482-cca1bffbd73e" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentAndFinanceLeaseRightOfUseAssetBeforeAccumulatedDepreciationAndAmortization_99c6bd4f-102d-4e60-8757-b2a77b7a622f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentAndFinanceLeaseRightOfUseAssetAccumulatedDepreciationAndAmortization_3612e67a-d111-4587-892b-fca7e17df587" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PropertyPlantAndEquipmentAndFinanceLeaseRightOfUseAssetAccumulatedDepreciationAndAmortization"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_5131c8ee-1ed7-49a2-a482-cca1bffbd73e" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentAndFinanceLeaseRightOfUseAssetAccumulatedDepreciationAndAmortization_3612e67a-d111-4587-892b-fca7e17df587" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentAndFinanceLeaseRightOfUseAssetAfterAccumulatedDepreciationAndAmortization_4edcf550-102e-4d9e-80a3-177e5831d339" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PropertyPlantAndEquipmentAndFinanceLeaseRightOfUseAssetAfterAccumulatedDepreciationAndAmortization"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_5131c8ee-1ed7-49a2-a482-cca1bffbd73e" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentAndFinanceLeaseRightOfUseAssetAfterAccumulatedDepreciationAndAmortization_4edcf550-102e-4d9e-80a3-177e5831d339" xlink:type="arc" order="4"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.workiva.com/role/FairValueMeasurementsDetails" xlink:type="simple" xlink:href="wk-20241231.xsd#FairValueMeasurementsDetails"/>
  <link:definitionLink xlink:role="http://www.workiva.com/role/FairValueMeasurementsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_d6177143-7f48-4b04-ad4c-2e12ce1aed59" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_a523949b-4284-4031-992f-1aa2cc39c397" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_d6177143-7f48-4b04-ad4c-2e12ce1aed59" xlink:to="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_a523949b-4284-4031-992f-1aa2cc39c397" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByMeasurementFrequencyAxis_942f2571-119d-47de-ac10-f407ba50b6fc" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueByMeasurementFrequencyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_a523949b-4284-4031-992f-1aa2cc39c397" xlink:to="loc_us-gaap_FairValueByMeasurementFrequencyAxis_942f2571-119d-47de-ac10-f407ba50b6fc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementFrequencyDomain_942f2571-119d-47de-ac10-f407ba50b6fc_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueMeasurementFrequencyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByMeasurementFrequencyAxis_942f2571-119d-47de-ac10-f407ba50b6fc" xlink:to="loc_us-gaap_FairValueMeasurementFrequencyDomain_942f2571-119d-47de-ac10-f407ba50b6fc_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementFrequencyDomain_6ccac433-859b-47ac-824c-48e563d21e89" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueMeasurementFrequencyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByMeasurementFrequencyAxis_942f2571-119d-47de-ac10-f407ba50b6fc" xlink:to="loc_us-gaap_FairValueMeasurementFrequencyDomain_6ccac433-859b-47ac-824c-48e563d21e89" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsRecurringMember_3996c697-a503-4c76-8ffb-5feae29bc112" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueMeasurementsRecurringMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementFrequencyDomain_6ccac433-859b-47ac-824c-48e563d21e89" xlink:to="loc_us-gaap_FairValueMeasurementsRecurringMember_3996c697-a503-4c76-8ffb-5feae29bc112" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_86817675-9bd7-40d4-beae-d78cb4124b68" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueByFairValueHierarchyLevelAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_a523949b-4284-4031-992f-1aa2cc39c397" xlink:to="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_86817675-9bd7-40d4-beae-d78cb4124b68" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_86817675-9bd7-40d4-beae-d78cb4124b68_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_86817675-9bd7-40d4-beae-d78cb4124b68" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_86817675-9bd7-40d4-beae-d78cb4124b68_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_bc9c4a0e-dd0f-4e36-a6b7-84d6ddbf296d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_86817675-9bd7-40d4-beae-d78cb4124b68" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_bc9c4a0e-dd0f-4e36-a6b7-84d6ddbf296d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel1Member_9610bf6c-2fe6-4a4d-8f9e-c0984450aa9e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueInputsLevel1Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_bc9c4a0e-dd0f-4e36-a6b7-84d6ddbf296d" xlink:to="loc_us-gaap_FairValueInputsLevel1Member_9610bf6c-2fe6-4a4d-8f9e-c0984450aa9e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel2Member_9486e754-04c7-494a-8610-c6a1deebf032" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueInputsLevel2Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_bc9c4a0e-dd0f-4e36-a6b7-84d6ddbf296d" xlink:to="loc_us-gaap_FairValueInputsLevel2Member_9486e754-04c7-494a-8610-c6a1deebf032" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsAxis_ffa84a9a-67b8-447b-b83e-e1f0149f5253" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CashAndCashEquivalentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_a523949b-4284-4031-992f-1aa2cc39c397" xlink:to="loc_us-gaap_CashAndCashEquivalentsAxis_ffa84a9a-67b8-447b-b83e-e1f0149f5253" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_ffa84a9a-67b8-447b-b83e-e1f0149f5253_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_CashAndCashEquivalentsAxis_ffa84a9a-67b8-447b-b83e-e1f0149f5253" xlink:to="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_ffa84a9a-67b8-447b-b83e-e1f0149f5253_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_0471a88d-6671-4978-b762-074d74048aed" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_CashAndCashEquivalentsAxis_ffa84a9a-67b8-447b-b83e-e1f0149f5253" xlink:to="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_0471a88d-6671-4978-b762-074d74048aed" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MoneyMarketFundsMember_bca22ddb-1661-4c30-bc90-ea16feea9cce" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_MoneyMarketFundsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_0471a88d-6671-4978-b762-074d74048aed" xlink:to="loc_us-gaap_MoneyMarketFundsMember_bca22ddb-1661-4c30-bc90-ea16feea9cce" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashEquivalentsMember_aee29eb8-925e-43d7-9bf1-2fcea08ea2f5" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CashEquivalentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_0471a88d-6671-4978-b762-074d74048aed" xlink:to="loc_us-gaap_CashEquivalentsMember_aee29eb8-925e-43d7-9bf1-2fcea08ea2f5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentAxis_2c52e9af-533f-419f-87d5-5a12f63efba1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FinancialInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_a523949b-4284-4031-992f-1aa2cc39c397" xlink:to="loc_us-gaap_FinancialInstrumentAxis_2c52e9af-533f-419f-87d5-5a12f63efba1" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_2c52e9af-533f-419f-87d5-5a12f63efba1_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancialInstrumentAxis_2c52e9af-533f-419f-87d5-5a12f63efba1" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_2c52e9af-533f-419f-87d5-5a12f63efba1_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_8c055a2b-a3b8-43b7-abe4-7bd646ed46ec" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancialInstrumentAxis_2c52e9af-533f-419f-87d5-5a12f63efba1" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_8c055a2b-a3b8-43b7-abe4-7bd646ed46ec" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommercialPaperMember_c9f5494f-de74-4844-8997-a2e4de5897c1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommercialPaperMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_8c055a2b-a3b8-43b7-abe4-7bd646ed46ec" xlink:to="loc_us-gaap_CommercialPaperMember_c9f5494f-de74-4844-8997-a2e4de5897c1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USTreasurySecuritiesMember_8d189344-cffe-4236-9f19-5c0c6232a6e6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_USTreasurySecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_8c055a2b-a3b8-43b7-abe4-7bd646ed46ec" xlink:to="loc_us-gaap_USTreasurySecuritiesMember_8d189344-cffe-4236-9f19-5c0c6232a6e6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USGovernmentAgenciesDebtSecuritiesMember_9a7197d4-1d9c-4235-a64f-59a66b5e83f8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_USGovernmentAgenciesDebtSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_8c055a2b-a3b8-43b7-abe4-7bd646ed46ec" xlink:to="loc_us-gaap_USGovernmentAgenciesDebtSecuritiesMember_9a7197d4-1d9c-4235-a64f-59a66b5e83f8" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CorporateDebtSecuritiesMember_0660ff3b-6f50-4473-bfe6-a57742e751cf" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CorporateDebtSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_8c055a2b-a3b8-43b7-abe4-7bd646ed46ec" xlink:to="loc_us-gaap_CorporateDebtSecuritiesMember_0660ff3b-6f50-4473-bfe6-a57742e751cf" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ForeignGovernmentDebtSecuritiesMember_966e0996-d09d-4486-9e69-17f18f87c0b1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ForeignGovernmentDebtSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_8c055a2b-a3b8-43b7-abe4-7bd646ed46ec" xlink:to="loc_us-gaap_ForeignGovernmentDebtSecuritiesMember_966e0996-d09d-4486-9e69-17f18f87c0b1" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_5e04fa35-0c17-4f41-84eb-d749f562f072" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_a523949b-4284-4031-992f-1aa2cc39c397" xlink:to="loc_us-gaap_DebtInstrumentAxis_5e04fa35-0c17-4f41-84eb-d749f562f072" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_5e04fa35-0c17-4f41-84eb-d749f562f072_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_5e04fa35-0c17-4f41-84eb-d749f562f072" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_5e04fa35-0c17-4f41-84eb-d749f562f072_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_a07372e6-14ab-4766-88a2-b81bb261cf3e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_5e04fa35-0c17-4f41-84eb-d749f562f072" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_a07372e6-14ab-4766-88a2-b81bb261cf3e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_OnePointOneTwoFivePercentConvertibleSeniorNotesDue2026Member_2469f081-1dff-4a4e-ac74-5c60ed6965bd" xlink:href="wk-20241231.xsd#wk_OnePointOneTwoFivePercentConvertibleSeniorNotesDue2026Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_a07372e6-14ab-4766-88a2-b81bb261cf3e" xlink:to="loc_wk_OnePointOneTwoFivePercentConvertibleSeniorNotesDue2026Member_2469f081-1dff-4a4e-ac74-5c60ed6965bd" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_OnePointTwoFivePercentConvertibleSeniorNotesDue2028Member_2e88cf19-074a-45e1-bb29-d0fe38e00c65" xlink:href="wk-20241231.xsd#wk_OnePointTwoFivePercentConvertibleSeniorNotesDue2028Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_a07372e6-14ab-4766-88a2-b81bb261cf3e" xlink:to="loc_wk_OnePointTwoFivePercentConvertibleSeniorNotesDue2028Member_2e88cf19-074a-45e1-bb29-d0fe38e00c65" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsFairValueDisclosure_fc81399b-dd37-4079-bd44-2ae7c92d8f0f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CashAndCashEquivalentsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_d6177143-7f48-4b04-ad4c-2e12ce1aed59" xlink:to="loc_us-gaap_CashAndCashEquivalentsFairValueDisclosure_fc81399b-dd37-4079-bd44-2ae7c92d8f0f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_67350ace-1dd8-4890-a992-b58d17cb0e77" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AvailableForSaleSecuritiesDebtSecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_d6177143-7f48-4b04-ad4c-2e12ce1aed59" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_67350ace-1dd8-4890-a992-b58d17cb0e77" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_CashCashEquivalentsAndAvailableForSaleDebtSecurities_87aa3b81-7ba8-487b-8998-2426f0545778" xlink:href="wk-20241231.xsd#wk_CashCashEquivalentsAndAvailableForSaleDebtSecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_d6177143-7f48-4b04-ad4c-2e12ce1aed59" xlink:to="loc_wk_CashCashEquivalentsAndAvailableForSaleDebtSecurities_87aa3b81-7ba8-487b-8998-2426f0545778" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsFairValueDisclosure_50557295-8965-44a3-9fa5-9eedc551bdda" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AssetsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_d6177143-7f48-4b04-ad4c-2e12ce1aed59" xlink:to="loc_us-gaap_AssetsFairValueDisclosure_50557295-8965-44a3-9fa5-9eedc551bdda" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConvertibleDebtFairValueDisclosures_3081d963-bca0-4378-8e5d-7e086926bce6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ConvertibleDebtFairValueDisclosures"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_d6177143-7f48-4b04-ad4c-2e12ce1aed59" xlink:to="loc_us-gaap_ConvertibleDebtFairValueDisclosures_3081d963-bca0-4378-8e5d-7e086926bce6" xlink:type="arc" order="4"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.workiva.com/role/CommitmentsandContingenciesDetails" xlink:type="simple" xlink:href="wk-20241231.xsd#CommitmentsandContingenciesDetails"/>
  <link:definitionLink xlink:role="http://www.workiva.com/role/CommitmentsandContingenciesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UnrecordedUnconditionalPurchaseObligationLineItems_ba1db641-9b98-409e-9d09-298eb7d9a085" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_UnrecordedUnconditionalPurchaseObligationLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UnrecordedUnconditionalPurchaseObligationTable_6575c1a2-e486-4faa-a6fd-a54fa9c2c107" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_UnrecordedUnconditionalPurchaseObligationTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_UnrecordedUnconditionalPurchaseObligationLineItems_ba1db641-9b98-409e-9d09-298eb7d9a085" xlink:to="loc_us-gaap_UnrecordedUnconditionalPurchaseObligationTable_6575c1a2-e486-4faa-a6fd-a54fa9c2c107" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UnrecordedUnconditionalPurchaseObligationByCategoryOfItemPurchasedAxis_fe01276a-fd48-4d9e-beca-78901cd6b9f3" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_UnrecordedUnconditionalPurchaseObligationByCategoryOfItemPurchasedAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_UnrecordedUnconditionalPurchaseObligationTable_6575c1a2-e486-4faa-a6fd-a54fa9c2c107" xlink:to="loc_us-gaap_UnrecordedUnconditionalPurchaseObligationByCategoryOfItemPurchasedAxis_fe01276a-fd48-4d9e-beca-78901cd6b9f3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UnconditionalPurchaseObligationCategoryOfGoodsOrServicesAcquiredDomain_fe01276a-fd48-4d9e-beca-78901cd6b9f3_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_UnconditionalPurchaseObligationCategoryOfGoodsOrServicesAcquiredDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_UnrecordedUnconditionalPurchaseObligationByCategoryOfItemPurchasedAxis_fe01276a-fd48-4d9e-beca-78901cd6b9f3" xlink:to="loc_us-gaap_UnconditionalPurchaseObligationCategoryOfGoodsOrServicesAcquiredDomain_fe01276a-fd48-4d9e-beca-78901cd6b9f3_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UnconditionalPurchaseObligationCategoryOfGoodsOrServicesAcquiredDomain_1771848f-5925-4d53-8141-790c2b1320de" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_UnconditionalPurchaseObligationCategoryOfGoodsOrServicesAcquiredDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_UnrecordedUnconditionalPurchaseObligationByCategoryOfItemPurchasedAxis_fe01276a-fd48-4d9e-beca-78901cd6b9f3" xlink:to="loc_us-gaap_UnconditionalPurchaseObligationCategoryOfGoodsOrServicesAcquiredDomain_1771848f-5925-4d53-8141-790c2b1320de" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_CloudServicesArrangementMember_7c30a3ae-7da1-49b6-b503-e08552053ac5" xlink:href="wk-20241231.xsd#wk_CloudServicesArrangementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_UnconditionalPurchaseObligationCategoryOfGoodsOrServicesAcquiredDomain_1771848f-5925-4d53-8141-790c2b1320de" xlink:to="loc_wk_CloudServicesArrangementMember_7c30a3ae-7da1-49b6-b503-e08552053ac5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UnrecordedUnconditionalPurchaseObligationBalanceOnFirstAnniversary_a41092bf-b2af-4262-8c8d-27b546a9b4de" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_UnrecordedUnconditionalPurchaseObligationBalanceOnFirstAnniversary"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_UnrecordedUnconditionalPurchaseObligationLineItems_ba1db641-9b98-409e-9d09-298eb7d9a085" xlink:to="loc_us-gaap_UnrecordedUnconditionalPurchaseObligationBalanceOnFirstAnniversary_a41092bf-b2af-4262-8c8d-27b546a9b4de" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UnrecordedUnconditionalPurchaseObligationBalanceOnSecondAnniversary_4f79a5af-eb87-4ad0-8194-97e853c69c7f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_UnrecordedUnconditionalPurchaseObligationBalanceOnSecondAnniversary"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_UnrecordedUnconditionalPurchaseObligationLineItems_ba1db641-9b98-409e-9d09-298eb7d9a085" xlink:to="loc_us-gaap_UnrecordedUnconditionalPurchaseObligationBalanceOnSecondAnniversary_4f79a5af-eb87-4ad0-8194-97e853c69c7f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UnrecordedUnconditionalPurchaseObligationBalanceOnThirdAnniversary_a0754f8c-d75f-4476-8f47-c8d38def0495" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_UnrecordedUnconditionalPurchaseObligationBalanceOnThirdAnniversary"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_UnrecordedUnconditionalPurchaseObligationLineItems_ba1db641-9b98-409e-9d09-298eb7d9a085" xlink:to="loc_us-gaap_UnrecordedUnconditionalPurchaseObligationBalanceOnThirdAnniversary_a0754f8c-d75f-4476-8f47-c8d38def0495" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UnrecordedUnconditionalPurchaseObligationBalanceOnFourthAnniversary_a7751736-ab70-42c7-9a06-79ab57414048" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_UnrecordedUnconditionalPurchaseObligationBalanceOnFourthAnniversary"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_UnrecordedUnconditionalPurchaseObligationLineItems_ba1db641-9b98-409e-9d09-298eb7d9a085" xlink:to="loc_us-gaap_UnrecordedUnconditionalPurchaseObligationBalanceOnFourthAnniversary_a7751736-ab70-42c7-9a06-79ab57414048" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UnrecordedUnconditionalPurchaseObligationBalanceOnFifthAnniversary_256fcc2e-4bcd-4177-b21d-4439283ca8db" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_UnrecordedUnconditionalPurchaseObligationBalanceOnFifthAnniversary"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_UnrecordedUnconditionalPurchaseObligationLineItems_ba1db641-9b98-409e-9d09-298eb7d9a085" xlink:to="loc_us-gaap_UnrecordedUnconditionalPurchaseObligationBalanceOnFifthAnniversary_256fcc2e-4bcd-4177-b21d-4439283ca8db" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UnrecordedUnconditionalPurchaseObligationBalanceSheetAmount_1d876893-c0f7-4112-b9e8-0e75b0714e0d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_UnrecordedUnconditionalPurchaseObligationBalanceSheetAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_UnrecordedUnconditionalPurchaseObligationLineItems_ba1db641-9b98-409e-9d09-298eb7d9a085" xlink:to="loc_us-gaap_UnrecordedUnconditionalPurchaseObligationBalanceSheetAmount_1d876893-c0f7-4112-b9e8-0e75b0714e0d" xlink:type="arc" order="5"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.workiva.com/role/LeasesNarrativeDetails" xlink:type="simple" xlink:href="wk-20241231.xsd#LeasesNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.workiva.com/role/LeasesNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeLeaseDescriptionLineItems_e99282be-a314-41fd-a766-85431d3042fb" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LesseeLeaseDescriptionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeLeaseDescriptionTable_46cb54d7-8a6f-4828-a8f1-4037be399a5d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LesseeLeaseDescriptionTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_LesseeLeaseDescriptionLineItems_e99282be-a314-41fd-a766-85431d3042fb" xlink:to="loc_us-gaap_LesseeLeaseDescriptionTable_46cb54d7-8a6f-4828-a8f1-4037be399a5d" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_87c432ac-ddab-4f63-9f0b-504d51a13273" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PropertyPlantAndEquipmentByTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LesseeLeaseDescriptionTable_46cb54d7-8a6f-4828-a8f1-4037be399a5d" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_87c432ac-ddab-4f63-9f0b-504d51a13273" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_87c432ac-ddab-4f63-9f0b-504d51a13273_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_87c432ac-ddab-4f63-9f0b-504d51a13273" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_87c432ac-ddab-4f63-9f0b-504d51a13273_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_176e288b-9a79-4244-8a6d-e46e61bb8879" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_87c432ac-ddab-4f63-9f0b-504d51a13273" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_176e288b-9a79-4244-8a6d-e46e61bb8879" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BuildingMember_19a9de06-b5e7-4a80-9164-dc6b7bf17211" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BuildingMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_176e288b-9a79-4244-8a6d-e46e61bb8879" xlink:to="loc_us-gaap_BuildingMember_19a9de06-b5e7-4a80-9164-dc6b7bf17211" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_264402a1-6d19-4e7f-9330-fc9d6df0645c" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LesseeLeaseDescriptionTable_46cb54d7-8a6f-4828-a8f1-4037be399a5d" xlink:to="loc_srt_RangeAxis_264402a1-6d19-4e7f-9330-fc9d6df0645c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_264402a1-6d19-4e7f-9330-fc9d6df0645c_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_264402a1-6d19-4e7f-9330-fc9d6df0645c" xlink:to="loc_srt_RangeMember_264402a1-6d19-4e7f-9330-fc9d6df0645c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_ac95dffe-e395-468b-b949-829eaa5e39fe" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_264402a1-6d19-4e7f-9330-fc9d6df0645c" xlink:to="loc_srt_RangeMember_ac95dffe-e395-468b-b949-829eaa5e39fe" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_dc8a3cd8-51de-42b6-aa61-05bccb430d41" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_ac95dffe-e395-468b-b949-829eaa5e39fe" xlink:to="loc_srt_MinimumMember_dc8a3cd8-51de-42b6-aa61-05bccb430d41" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_a72e0a23-fd8d-4ad5-8bca-a8bfc643f038" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_ac95dffe-e395-468b-b949-829eaa5e39fe" xlink:to="loc_srt_MaximumMember_a72e0a23-fd8d-4ad5-8bca-a8bfc643f038" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_LesseeOperatingLeaseNumberOfRenewalOptions_6ee16414-6c73-4995-b690-3baeee903868" xlink:href="wk-20241231.xsd#wk_LesseeOperatingLeaseNumberOfRenewalOptions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LesseeLeaseDescriptionLineItems_e99282be-a314-41fd-a766-85431d3042fb" xlink:to="loc_wk_LesseeOperatingLeaseNumberOfRenewalOptions_6ee16414-6c73-4995-b690-3baeee903868" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseRenewalTerm_ced53636-d8e3-4e0a-addd-46bba16b9626" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LesseeOperatingLeaseRenewalTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LesseeLeaseDescriptionLineItems_e99282be-a314-41fd-a766-85431d3042fb" xlink:to="loc_us-gaap_LesseeOperatingLeaseRenewalTerm_ced53636-d8e3-4e0a-addd-46bba16b9626" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.workiva.com/role/DebtSummaryofConvertibleDebtDetails" xlink:type="simple" xlink:href="wk-20241231.xsd#DebtSummaryofConvertibleDebtDetails"/>
  <link:definitionLink xlink:role="http://www.workiva.com/role/DebtSummaryofConvertibleDebtDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentLineItems_9e64a32b-08ff-4399-82f8-21fc5218241a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_a40054db-5317-45ce-9973-a4c47b0756ad" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DebtInstrumentLineItems_9e64a32b-08ff-4399-82f8-21fc5218241a" xlink:to="loc_us-gaap_DebtInstrumentTable_a40054db-5317-45ce-9973-a4c47b0756ad" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_c66edbc9-e79c-4843-bb0c-6d4c949b6e7e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_a40054db-5317-45ce-9973-a4c47b0756ad" xlink:to="loc_us-gaap_DebtInstrumentAxis_c66edbc9-e79c-4843-bb0c-6d4c949b6e7e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_c66edbc9-e79c-4843-bb0c-6d4c949b6e7e_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_c66edbc9-e79c-4843-bb0c-6d4c949b6e7e" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_c66edbc9-e79c-4843-bb0c-6d4c949b6e7e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_b5d05282-c151-40f2-bd78-07628539b6cd" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_c66edbc9-e79c-4843-bb0c-6d4c949b6e7e" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_b5d05282-c151-40f2-bd78-07628539b6cd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_OnePointOneTwoFivePercentConvertibleSeniorNotesDue2026Member_2975697d-74aa-49d4-b860-c837a0fc080a" xlink:href="wk-20241231.xsd#wk_OnePointOneTwoFivePercentConvertibleSeniorNotesDue2026Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_b5d05282-c151-40f2-bd78-07628539b6cd" xlink:to="loc_wk_OnePointOneTwoFivePercentConvertibleSeniorNotesDue2026Member_2975697d-74aa-49d4-b860-c837a0fc080a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_OnePointTwoFivePercentConvertibleSeniorNotesDue2028Member_96bc1fb9-6b4f-4455-9ab8-9f0d93944f68" xlink:href="wk-20241231.xsd#wk_OnePointTwoFivePercentConvertibleSeniorNotesDue2028Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_b5d05282-c151-40f2-bd78-07628539b6cd" xlink:to="loc_wk_OnePointTwoFivePercentConvertibleSeniorNotesDue2028Member_96bc1fb9-6b4f-4455-9ab8-9f0d93944f68" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_ae0525d3-b9fa-4bde-9692-dde6bd7ad39e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_a40054db-5317-45ce-9973-a4c47b0756ad" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_ae0525d3-b9fa-4bde-9692-dde6bd7ad39e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_ae0525d3-b9fa-4bde-9692-dde6bd7ad39e_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_ae0525d3-b9fa-4bde-9692-dde6bd7ad39e" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_ae0525d3-b9fa-4bde-9692-dde6bd7ad39e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_0fe79a3c-b5b6-426d-b393-12ad0f51626e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_ae0525d3-b9fa-4bde-9692-dde6bd7ad39e" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_0fe79a3c-b5b6-426d-b393-12ad0f51626e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConvertibleDebtMember_d3678c9e-2e53-4b01-84f0-4dd53f13ee66" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ConvertibleDebtMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_0fe79a3c-b5b6-426d-b393-12ad0f51626e" xlink:to="loc_us-gaap_ConvertibleDebtMember_d3678c9e-2e53-4b01-84f0-4dd53f13ee66" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentFaceAmount_68fbb11f-0ba8-4f57-ae6c-c950d7df286c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentFaceAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_9e64a32b-08ff-4399-82f8-21fc5218241a" xlink:to="loc_us-gaap_DebtInstrumentFaceAmount_68fbb11f-0ba8-4f57-ae6c-c950d7df286c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentConvertibleConversionRatio1_052ada96-e8af-4d03-95e7-1ccb765c40f4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentConvertibleConversionRatio1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_9e64a32b-08ff-4399-82f8-21fc5218241a" xlink:to="loc_us-gaap_DebtInstrumentConvertibleConversionRatio1_052ada96-e8af-4d03-95e7-1ccb765c40f4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentConvertibleConversionPrice1_c0a46797-d678-4c9d-9f12-f8bb6e903ce6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentConvertibleConversionPrice1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_9e64a32b-08ff-4399-82f8-21fc5218241a" xlink:to="loc_us-gaap_DebtInstrumentConvertibleConversionPrice1_c0a46797-d678-4c9d-9f12-f8bb6e903ce6" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.workiva.com/role/DebtNarrativeDetails" xlink:type="simple" xlink:href="wk-20241231.xsd#DebtNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.workiva.com/role/DebtNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentLineItems_867c6202-672f-406c-ae21-b0804a5ae51c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_f3aa61b7-5114-4ca5-8b1f-5f8607544b2a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DebtInstrumentLineItems_867c6202-672f-406c-ae21-b0804a5ae51c" xlink:to="loc_us-gaap_DebtInstrumentTable_f3aa61b7-5114-4ca5-8b1f-5f8607544b2a" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_ad76b8bd-7214-48fb-b498-25a15a43bc60" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_f3aa61b7-5114-4ca5-8b1f-5f8607544b2a" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_ad76b8bd-7214-48fb-b498-25a15a43bc60" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_ad76b8bd-7214-48fb-b498-25a15a43bc60_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_ad76b8bd-7214-48fb-b498-25a15a43bc60" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_ad76b8bd-7214-48fb-b498-25a15a43bc60_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_e7d5c2d2-4024-4ce2-8a82-e07f5ff8a44f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_ad76b8bd-7214-48fb-b498-25a15a43bc60" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_e7d5c2d2-4024-4ce2-8a82-e07f5ff8a44f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConvertibleDebtMember_c1093522-fa0f-4d80-af10-35f0923142e0" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ConvertibleDebtMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_e7d5c2d2-4024-4ce2-8a82-e07f5ff8a44f" xlink:to="loc_us-gaap_ConvertibleDebtMember_c1093522-fa0f-4d80-af10-35f0923142e0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_6dc9c37c-f325-47cd-97f1-63eba772ed2a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_f3aa61b7-5114-4ca5-8b1f-5f8607544b2a" xlink:to="loc_us-gaap_DebtInstrumentAxis_6dc9c37c-f325-47cd-97f1-63eba772ed2a" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_6dc9c37c-f325-47cd-97f1-63eba772ed2a_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_6dc9c37c-f325-47cd-97f1-63eba772ed2a" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_6dc9c37c-f325-47cd-97f1-63eba772ed2a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_5ccbe548-3b2e-437f-b745-d4f192b254b6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_6dc9c37c-f325-47cd-97f1-63eba772ed2a" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_5ccbe548-3b2e-437f-b745-d4f192b254b6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_OnePointOneTwoFivePercentConvertibleSeniorNotesDue2026Member_04ff59d8-2b02-4c45-96b2-59a82ece7e35" xlink:href="wk-20241231.xsd#wk_OnePointOneTwoFivePercentConvertibleSeniorNotesDue2026Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_5ccbe548-3b2e-437f-b745-d4f192b254b6" xlink:to="loc_wk_OnePointOneTwoFivePercentConvertibleSeniorNotesDue2026Member_04ff59d8-2b02-4c45-96b2-59a82ece7e35" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_OnePointOneTwoFivePercentConvertibleSeniorNotesDue2026OverAllotmentOptionMember_e1e43aad-fa6d-4d52-bfa7-67055e0162f4" xlink:href="wk-20241231.xsd#wk_OnePointOneTwoFivePercentConvertibleSeniorNotesDue2026OverAllotmentOptionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_wk_OnePointOneTwoFivePercentConvertibleSeniorNotesDue2026Member_04ff59d8-2b02-4c45-96b2-59a82ece7e35" xlink:to="loc_wk_OnePointOneTwoFivePercentConvertibleSeniorNotesDue2026OverAllotmentOptionMember_e1e43aad-fa6d-4d52-bfa7-67055e0162f4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_OnePointTwoFivePercentConvertibleSeniorNotesDue2028Member_95e760de-018a-4500-8f68-f92d9ec02a7a" xlink:href="wk-20241231.xsd#wk_OnePointTwoFivePercentConvertibleSeniorNotesDue2028Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_5ccbe548-3b2e-437f-b745-d4f192b254b6" xlink:to="loc_wk_OnePointTwoFivePercentConvertibleSeniorNotesDue2028Member_95e760de-018a-4500-8f68-f92d9ec02a7a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_OnePointOneTwoFivePercentConvertibleSeniorNotesDue2028OverAllotmentOptionMember_5e8c43d5-a8b0-4536-8e8f-cddf31c6338b" xlink:href="wk-20241231.xsd#wk_OnePointOneTwoFivePercentConvertibleSeniorNotesDue2028OverAllotmentOptionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_wk_OnePointTwoFivePercentConvertibleSeniorNotesDue2028Member_95e760de-018a-4500-8f68-f92d9ec02a7a" xlink:to="loc_wk_OnePointOneTwoFivePercentConvertibleSeniorNotesDue2028OverAllotmentOptionMember_5e8c43d5-a8b0-4536-8e8f-cddf31c6338b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_DebtInstrumentConvertibleTermsOfConversionAxis_87191fee-fefb-4f69-aa86-535895bf5107" xlink:href="wk-20241231.xsd#wk_DebtInstrumentConvertibleTermsOfConversionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_f3aa61b7-5114-4ca5-8b1f-5f8607544b2a" xlink:to="loc_wk_DebtInstrumentConvertibleTermsOfConversionAxis_87191fee-fefb-4f69-aa86-535895bf5107" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_DebtInstrumentConvertibleTermsOfConversionDomain_87191fee-fefb-4f69-aa86-535895bf5107_default" xlink:href="wk-20241231.xsd#wk_DebtInstrumentConvertibleTermsOfConversionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_wk_DebtInstrumentConvertibleTermsOfConversionAxis_87191fee-fefb-4f69-aa86-535895bf5107" xlink:to="loc_wk_DebtInstrumentConvertibleTermsOfConversionDomain_87191fee-fefb-4f69-aa86-535895bf5107_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_DebtInstrumentConvertibleTermsOfConversionDomain_5c2ad98e-b4ff-4401-a43d-455a24e5a559" xlink:href="wk-20241231.xsd#wk_DebtInstrumentConvertibleTermsOfConversionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_wk_DebtInstrumentConvertibleTermsOfConversionAxis_87191fee-fefb-4f69-aa86-535895bf5107" xlink:to="loc_wk_DebtInstrumentConvertibleTermsOfConversionDomain_5c2ad98e-b4ff-4401-a43d-455a24e5a559" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_DebtConversionTermsOneMember_169c428b-e0f2-4c56-b274-c30895466fb0" xlink:href="wk-20241231.xsd#wk_DebtConversionTermsOneMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_wk_DebtInstrumentConvertibleTermsOfConversionDomain_5c2ad98e-b4ff-4401-a43d-455a24e5a559" xlink:to="loc_wk_DebtConversionTermsOneMember_169c428b-e0f2-4c56-b274-c30895466fb0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_DebtConversionTermsTwoMember_08ecad0d-9ae5-418d-9c9a-1f78794ce700" xlink:href="wk-20241231.xsd#wk_DebtConversionTermsTwoMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_wk_DebtInstrumentConvertibleTermsOfConversionDomain_5c2ad98e-b4ff-4401-a43d-455a24e5a559" xlink:to="loc_wk_DebtConversionTermsTwoMember_08ecad0d-9ae5-418d-9c9a-1f78794ce700" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_06061191-78b9-4430-8307-827293873028" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_f3aa61b7-5114-4ca5-8b1f-5f8607544b2a" xlink:to="loc_us-gaap_StatementClassOfStockAxis_06061191-78b9-4430-8307-827293873028" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_06061191-78b9-4430-8307-827293873028_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_06061191-78b9-4430-8307-827293873028" xlink:to="loc_us-gaap_ClassOfStockDomain_06061191-78b9-4430-8307-827293873028_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_47a5ad85-3dce-42d0-8685-4256198e142c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_06061191-78b9-4430-8307-827293873028" xlink:to="loc_us-gaap_ClassOfStockDomain_47a5ad85-3dce-42d0-8685-4256198e142c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonClassAMember_2cdab126-33e5-4eb4-94e9-bf797e51edb3" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonClassAMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_47a5ad85-3dce-42d0-8685-4256198e142c" xlink:to="loc_us-gaap_CommonClassAMember_2cdab126-33e5-4eb4-94e9-bf797e51edb3" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentFaceAmount_9a23c67a-cd39-46d7-88c5-3f52295d9814" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentFaceAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_867c6202-672f-406c-ae21-b0804a5ae51c" xlink:to="loc_us-gaap_DebtInstrumentFaceAmount_9a23c67a-cd39-46d7-88c5-3f52295d9814" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_d12148cb-0c2c-4105-9e70-640d37a72a9a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentInterestRateStatedPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_867c6202-672f-406c-ae21-b0804a5ae51c" xlink:to="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_d12148cb-0c2c-4105-9e70-640d37a72a9a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromConvertibleDebt_c0e86976-dce1-4079-b8f6-8e85edb91589" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProceedsFromConvertibleDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_867c6202-672f-406c-ae21-b0804a5ae51c" xlink:to="loc_us-gaap_ProceedsFromConvertibleDebt_c0e86976-dce1-4079-b8f6-8e85edb91589" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_DebtInstrumentFaceAmountMaximumOverAllotmentOption_4263d461-3f5a-47b4-8780-e8ee356ef686" xlink:href="wk-20241231.xsd#wk_DebtInstrumentFaceAmountMaximumOverAllotmentOption"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_867c6202-672f-406c-ae21-b0804a5ae51c" xlink:to="loc_wk_DebtInstrumentFaceAmountMaximumOverAllotmentOption_4263d461-3f5a-47b4-8780-e8ee356ef686" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockParOrStatedValuePerShare_ed578335-3895-40b3-b727-95465585cbfe" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonStockParOrStatedValuePerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_867c6202-672f-406c-ae21-b0804a5ae51c" xlink:to="loc_us-gaap_CommonStockParOrStatedValuePerShare_ed578335-3895-40b3-b727-95465585cbfe" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentConvertibleThresholdTradingDays_0804e65d-7511-4701-9c1c-cc961cc1cbb0" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentConvertibleThresholdTradingDays"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_867c6202-672f-406c-ae21-b0804a5ae51c" xlink:to="loc_us-gaap_DebtInstrumentConvertibleThresholdTradingDays_0804e65d-7511-4701-9c1c-cc961cc1cbb0" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentConvertibleThresholdConsecutiveTradingDays1_a827f546-4879-48a3-92f1-392189d2f57f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentConvertibleThresholdConsecutiveTradingDays1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_867c6202-672f-406c-ae21-b0804a5ae51c" xlink:to="loc_us-gaap_DebtInstrumentConvertibleThresholdConsecutiveTradingDays1_a827f546-4879-48a3-92f1-392189d2f57f" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentConvertibleThresholdPercentageOfStockPriceTrigger_b13f5f75-b7df-4d23-a524-eb70d78bf3c8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentConvertibleThresholdPercentageOfStockPriceTrigger"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_867c6202-672f-406c-ae21-b0804a5ae51c" xlink:to="loc_us-gaap_DebtInstrumentConvertibleThresholdPercentageOfStockPriceTrigger_b13f5f75-b7df-4d23-a524-eb70d78bf3c8" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_DebtInstrumentConvertibleThresholdPercentageOfStockTradingPrice_eb68dbea-1cff-4d13-a7c7-fa5bd2b4900e" xlink:href="wk-20241231.xsd#wk_DebtInstrumentConvertibleThresholdPercentageOfStockTradingPrice"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_867c6202-672f-406c-ae21-b0804a5ae51c" xlink:to="loc_wk_DebtInstrumentConvertibleThresholdPercentageOfStockTradingPrice_eb68dbea-1cff-4d13-a7c7-fa5bd2b4900e" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentRedemptionPricePercentage_bfe7af93-f332-420b-8d35-117463ab72c0" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentRedemptionPricePercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_867c6202-672f-406c-ae21-b0804a5ae51c" xlink:to="loc_us-gaap_DebtInstrumentRedemptionPricePercentage_bfe7af93-f332-420b-8d35-117463ab72c0" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentInterestRateEffectivePercentage_a6403f23-9bec-4dcb-ac38-ef267eaedffd" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentInterestRateEffectivePercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_867c6202-672f-406c-ae21-b0804a5ae51c" xlink:to="loc_us-gaap_DebtInstrumentInterestRateEffectivePercentage_a6403f23-9bec-4dcb-ac38-ef267eaedffd" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_DebtInstrumentRemainingTerm_af3633db-0c2b-4e0f-9522-db81cb5790b6" xlink:href="wk-20241231.xsd#wk_DebtInstrumentRemainingTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_867c6202-672f-406c-ae21-b0804a5ae51c" xlink:to="loc_wk_DebtInstrumentRemainingTerm_af3633db-0c2b-4e0f-9522-db81cb5790b6" xlink:type="arc" order="11"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.workiva.com/role/DebtNetCarryingAmountofConvertibleDebtDetails" xlink:type="simple" xlink:href="wk-20241231.xsd#DebtNetCarryingAmountofConvertibleDebtDetails"/>
  <link:definitionLink xlink:role="http://www.workiva.com/role/DebtNetCarryingAmountofConvertibleDebtDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentLineItems_85934138-f8d3-4de8-b296-f4e45fa20f9e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_f17137a4-3f3e-4db6-9b28-0ac03c88339a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DebtInstrumentLineItems_85934138-f8d3-4de8-b296-f4e45fa20f9e" xlink:to="loc_us-gaap_DebtInstrumentTable_f17137a4-3f3e-4db6-9b28-0ac03c88339a" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_95725e57-b8ad-43a6-9680-e2f38d267a41" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_f17137a4-3f3e-4db6-9b28-0ac03c88339a" xlink:to="loc_us-gaap_DebtInstrumentAxis_95725e57-b8ad-43a6-9680-e2f38d267a41" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_95725e57-b8ad-43a6-9680-e2f38d267a41_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_95725e57-b8ad-43a6-9680-e2f38d267a41" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_95725e57-b8ad-43a6-9680-e2f38d267a41_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_d4246fc6-6336-443f-8178-49425a2c7a1f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_95725e57-b8ad-43a6-9680-e2f38d267a41" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_d4246fc6-6336-443f-8178-49425a2c7a1f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_OnePointOneTwoFivePercentConvertibleSeniorNotesDue2026Member_aaedfe33-d127-4d13-af7a-34277e7e6b70" xlink:href="wk-20241231.xsd#wk_OnePointOneTwoFivePercentConvertibleSeniorNotesDue2026Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_d4246fc6-6336-443f-8178-49425a2c7a1f" xlink:to="loc_wk_OnePointOneTwoFivePercentConvertibleSeniorNotesDue2026Member_aaedfe33-d127-4d13-af7a-34277e7e6b70" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_OnePointTwoFivePercentConvertibleSeniorNotesDue2028Member_00446546-16c7-4511-b70c-f1b0fcd696f9" xlink:href="wk-20241231.xsd#wk_OnePointTwoFivePercentConvertibleSeniorNotesDue2028Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_d4246fc6-6336-443f-8178-49425a2c7a1f" xlink:to="loc_wk_OnePointTwoFivePercentConvertibleSeniorNotesDue2028Member_00446546-16c7-4511-b70c-f1b0fcd696f9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_7fe51476-2587-424a-9906-614682407901" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_f17137a4-3f3e-4db6-9b28-0ac03c88339a" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_7fe51476-2587-424a-9906-614682407901" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_7fe51476-2587-424a-9906-614682407901_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_7fe51476-2587-424a-9906-614682407901" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_7fe51476-2587-424a-9906-614682407901_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_15d00b4e-bb02-47e6-82e3-9f6a66edfcb7" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_7fe51476-2587-424a-9906-614682407901" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_15d00b4e-bb02-47e6-82e3-9f6a66edfcb7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConvertibleDebtMember_05110738-58a6-48fd-8e2b-c6dd5d2e5722" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ConvertibleDebtMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_15d00b4e-bb02-47e6-82e3-9f6a66edfcb7" xlink:to="loc_us-gaap_ConvertibleDebtMember_05110738-58a6-48fd-8e2b-c6dd5d2e5722" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentCarryingAmount_3a2d867f-6625-4406-a987-901b9f290de9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentCarryingAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_85934138-f8d3-4de8-b296-f4e45fa20f9e" xlink:to="loc_us-gaap_DebtInstrumentCarryingAmount_3a2d867f-6625-4406-a987-901b9f290de9" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredFinanceCostsNet_f54f266d-2321-404d-b283-c3a651f9ee9a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DeferredFinanceCostsNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_85934138-f8d3-4de8-b296-f4e45fa20f9e" xlink:to="loc_us-gaap_DeferredFinanceCostsNet_f54f266d-2321-404d-b283-c3a651f9ee9a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebt_8e04b0f9-2824-4406-8263-468832cdcceb" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongTermDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_85934138-f8d3-4de8-b296-f4e45fa20f9e" xlink:to="loc_us-gaap_LongTermDebt_8e04b0f9-2824-4406-8263-468832cdcceb" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.workiva.com/role/StockholdersEquityDetails" xlink:type="simple" xlink:href="wk-20241231.xsd#StockholdersEquityDetails"/>
  <link:definitionLink xlink:role="http://www.workiva.com/role/StockholdersEquityDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockLineItems_67ad8723-748c-4228-8f92-e65088f499c0" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfStockByClassTable_b9c0ceac-0b81-461b-aa32-3f9efa31c775" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfStockByClassTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ClassOfStockLineItems_67ad8723-748c-4228-8f92-e65088f499c0" xlink:to="loc_us-gaap_ScheduleOfStockByClassTable_b9c0ceac-0b81-461b-aa32-3f9efa31c775" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_fc7b13cd-ed19-44d0-ad2d-b5f71d12aeb5" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfStockByClassTable_b9c0ceac-0b81-461b-aa32-3f9efa31c775" xlink:to="loc_us-gaap_StatementClassOfStockAxis_fc7b13cd-ed19-44d0-ad2d-b5f71d12aeb5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_fc7b13cd-ed19-44d0-ad2d-b5f71d12aeb5_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_fc7b13cd-ed19-44d0-ad2d-b5f71d12aeb5" xlink:to="loc_us-gaap_ClassOfStockDomain_fc7b13cd-ed19-44d0-ad2d-b5f71d12aeb5_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_8b53a484-b141-4243-bfee-2eef8a05c4b9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_fc7b13cd-ed19-44d0-ad2d-b5f71d12aeb5" xlink:to="loc_us-gaap_ClassOfStockDomain_8b53a484-b141-4243-bfee-2eef8a05c4b9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonClassAMember_6f60a98b-5348-42d3-9b03-202bdf09caac" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonClassAMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_8b53a484-b141-4243-bfee-2eef8a05c4b9" xlink:to="loc_us-gaap_CommonClassAMember_6f60a98b-5348-42d3-9b03-202bdf09caac" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonClassBMember_8e6bb85b-7433-4835-b856-ba0280b257bd" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonClassBMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_8b53a484-b141-4243-bfee-2eef8a05c4b9" xlink:to="loc_us-gaap_CommonClassBMember_8e6bb85b-7433-4835-b856-ba0280b257bd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_CommonStockVotingRightsVotesPerShare_30fd0f75-7e8e-454a-938d-83e17a678b9a" xlink:href="wk-20241231.xsd#wk_CommonStockVotingRightsVotesPerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_67ad8723-748c-4228-8f92-e65088f499c0" xlink:to="loc_wk_CommonStockVotingRightsVotesPerShare_30fd0f75-7e8e-454a-938d-83e17a678b9a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_CommonStockConversionBasisRatio_f3a2caef-1192-4899-85e2-e153a9e45a03" xlink:href="wk-20241231.xsd#wk_CommonStockConversionBasisRatio"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_67ad8723-748c-4228-8f92-e65088f499c0" xlink:to="loc_wk_CommonStockConversionBasisRatio_f3a2caef-1192-4899-85e2-e153a9e45a03" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.workiva.com/role/StockBasedCompensationGeneralDetails" xlink:type="simple" xlink:href="wk-20241231.xsd#StockBasedCompensationGeneralDetails"/>
  <link:definitionLink xlink:role="http://www.workiva.com/role/StockBasedCompensationGeneralDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_58cdd08a-6cb0-4ca6-934e-38faa941d2de" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_bd70b14e-11e9-4b29-aed0-c3d344ecd600" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_58cdd08a-6cb0-4ca6-934e-38faa941d2de" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_bd70b14e-11e9-4b29-aed0-c3d344ecd600" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameAxis_cdd929fc-f58c-4419-b2a9-14b6bed5f558" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PlanNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_bd70b14e-11e9-4b29-aed0-c3d344ecd600" xlink:to="loc_us-gaap_PlanNameAxis_cdd929fc-f58c-4419-b2a9-14b6bed5f558" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameDomain_cdd929fc-f58c-4419-b2a9-14b6bed5f558_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PlanNameAxis_cdd929fc-f58c-4419-b2a9-14b6bed5f558" xlink:to="loc_us-gaap_PlanNameDomain_cdd929fc-f58c-4419-b2a9-14b6bed5f558_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameDomain_e5d7eb09-3024-4044-b074-0062be0721f1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PlanNameAxis_cdd929fc-f58c-4419-b2a9-14b6bed5f558" xlink:to="loc_us-gaap_PlanNameDomain_e5d7eb09-3024-4044-b074-0062be0721f1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_EquityIncentivePlan2014Member_690e7efb-4c21-409a-8386-9f1fc6acb7a1" xlink:href="wk-20241231.xsd#wk_EquityIncentivePlan2014Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanNameDomain_e5d7eb09-3024-4044-b074-0062be0721f1" xlink:to="loc_wk_EquityIncentivePlan2014Member_690e7efb-4c21-409a-8386-9f1fc6acb7a1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_5fd2b37a-a466-45ab-8939-97676a4495af" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_bd70b14e-11e9-4b29-aed0-c3d344ecd600" xlink:to="loc_us-gaap_AwardTypeAxis_5fd2b37a-a466-45ab-8939-97676a4495af" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_5fd2b37a-a466-45ab-8939-97676a4495af_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_5fd2b37a-a466-45ab-8939-97676a4495af" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_5fd2b37a-a466-45ab-8939-97676a4495af_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_1b6fb515-4fc8-4dd5-aef2-14ae14fec156" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_5fd2b37a-a466-45ab-8939-97676a4495af" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_1b6fb515-4fc8-4dd5-aef2-14ae14fec156" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockMember_77c9686f-7aa8-46f1-a4be-72edaf62983a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EmployeeStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_1b6fb515-4fc8-4dd5-aef2-14ae14fec156" xlink:to="loc_us-gaap_EmployeeStockMember_77c9686f-7aa8-46f1-a4be-72edaf62983a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_354ce9b9-29b3-4590-90da-614b90710df0" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_bd70b14e-11e9-4b29-aed0-c3d344ecd600" xlink:to="loc_us-gaap_StatementClassOfStockAxis_354ce9b9-29b3-4590-90da-614b90710df0" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_354ce9b9-29b3-4590-90da-614b90710df0_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_354ce9b9-29b3-4590-90da-614b90710df0" xlink:to="loc_us-gaap_ClassOfStockDomain_354ce9b9-29b3-4590-90da-614b90710df0_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_627ab757-e99a-472d-9680-087b0e6742eb" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_354ce9b9-29b3-4590-90da-614b90710df0" xlink:to="loc_us-gaap_ClassOfStockDomain_627ab757-e99a-472d-9680-087b0e6742eb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonClassAMember_6a8ab38f-137a-4e00-9900-cd74983a755d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonClassAMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_627ab757-e99a-472d-9680-087b0e6742eb" xlink:to="loc_us-gaap_CommonClassAMember_6a8ab38f-137a-4e00-9900-cd74983a755d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAvailableForGrant_ec0abe19-945f-4cc7-84b7-c25409e59092" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAvailableForGrant"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_58cdd08a-6cb0-4ca6-934e-38faa941d2de" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAvailableForGrant_ec0abe19-945f-4cc7-84b7-c25409e59092" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardPurchasePriceOfCommonStockPercent_b6645f25-5cd6-4ddb-9890-1ab8b6183a0b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardPurchasePriceOfCommonStockPercent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_58cdd08a-6cb0-4ca6-934e-38faa941d2de" xlink:to="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardPurchasePriceOfCommonStockPercent_b6645f25-5cd6-4ddb-9890-1ab8b6183a0b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_SharebasedCompensationArrangementBySharebasedPaymentAwardMaximumPurchaseValueDuringOfferingPeriodPerEmployee_edb28537-2947-4872-be12-0e4fe318120c" xlink:href="wk-20241231.xsd#wk_SharebasedCompensationArrangementBySharebasedPaymentAwardMaximumPurchaseValueDuringOfferingPeriodPerEmployee"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_58cdd08a-6cb0-4ca6-934e-38faa941d2de" xlink:to="loc_wk_SharebasedCompensationArrangementBySharebasedPaymentAwardMaximumPurchaseValueDuringOfferingPeriodPerEmployee_edb28537-2947-4872-be12-0e4fe318120c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_SharebasedCompensationArrangementBySharebasedPaymentAwardStockPlanOfferingPeriod_56274a38-b1b2-42c6-9a16-1e381c000dd8" xlink:href="wk-20241231.xsd#wk_SharebasedCompensationArrangementBySharebasedPaymentAwardStockPlanOfferingPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_58cdd08a-6cb0-4ca6-934e-38faa941d2de" xlink:to="loc_wk_SharebasedCompensationArrangementBySharebasedPaymentAwardStockPlanOfferingPeriod_56274a38-b1b2-42c6-9a16-1e381c000dd8" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.workiva.com/role/StockBasedCompensationStockBasedCompensationExpenseDetails" xlink:type="simple" xlink:href="wk-20241231.xsd#StockBasedCompensationStockBasedCompensationExpenseDetails"/>
  <link:definitionLink xlink:role="http://www.workiva.com/role/StockBasedCompensationStockBasedCompensationExpenseDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems_812d5762-6cf0-43b5-85fc-71d43218c7b3" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfEmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsTable_b3258a4a-c457-4262-9451-d53bd0e6f720" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfEmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems_812d5762-6cf0-43b5-85fc-71d43218c7b3" xlink:to="loc_us-gaap_ScheduleOfEmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsTable_b3258a4a-c457-4262-9451-d53bd0e6f720" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductOrServiceAxis_e4b42c9a-f4bc-4603-881d-f98c94f92dee" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ProductOrServiceAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfEmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsTable_b3258a4a-c457-4262-9451-d53bd0e6f720" xlink:to="loc_srt_ProductOrServiceAxis_e4b42c9a-f4bc-4603-881d-f98c94f92dee" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_e4b42c9a-f4bc-4603-881d-f98c94f92dee_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ProductOrServiceAxis_e4b42c9a-f4bc-4603-881d-f98c94f92dee" xlink:to="loc_srt_ProductsAndServicesDomain_e4b42c9a-f4bc-4603-881d-f98c94f92dee_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_1b2fc829-a963-4029-bcd7-475f2e5f878c" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ProductOrServiceAxis_e4b42c9a-f4bc-4603-881d-f98c94f92dee" xlink:to="loc_srt_ProductsAndServicesDomain_1b2fc829-a963-4029-bcd7-475f2e5f878c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LicenseAndServiceMember_f60bb3c4-8356-433e-877c-f7a4e8e38748" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LicenseAndServiceMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_1b2fc829-a963-4029-bcd7-475f2e5f878c" xlink:to="loc_us-gaap_LicenseAndServiceMember_f60bb3c4-8356-433e-877c-f7a4e8e38748" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_ProfessionalServicesMember_2e38c542-90a8-4f7b-bed5-26676e487f12" xlink:href="wk-20241231.xsd#wk_ProfessionalServicesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_1b2fc829-a963-4029-bcd7-475f2e5f878c" xlink:to="loc_wk_ProfessionalServicesMember_2e38c542-90a8-4f7b-bed5-26676e487f12" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationAxis_d3aea538-28cb-4dab-aa93-fce3b0e39fa2" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncomeStatementLocationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfEmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsTable_b3258a4a-c457-4262-9451-d53bd0e6f720" xlink:to="loc_us-gaap_IncomeStatementLocationAxis_d3aea538-28cb-4dab-aa93-fce3b0e39fa2" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationDomain_d3aea538-28cb-4dab-aa93-fce3b0e39fa2_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncomeStatementLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_IncomeStatementLocationAxis_d3aea538-28cb-4dab-aa93-fce3b0e39fa2" xlink:to="loc_us-gaap_IncomeStatementLocationDomain_d3aea538-28cb-4dab-aa93-fce3b0e39fa2_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationDomain_aea2172e-11f0-42ce-9b4f-6cfe2c55051e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncomeStatementLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_IncomeStatementLocationAxis_d3aea538-28cb-4dab-aa93-fce3b0e39fa2" xlink:to="loc_us-gaap_IncomeStatementLocationDomain_aea2172e-11f0-42ce-9b4f-6cfe2c55051e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostOfSalesMember_9b8eb43b-f729-4d23-b9f7-9341ef38576b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CostOfSalesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_aea2172e-11f0-42ce-9b4f-6cfe2c55051e" xlink:to="loc_us-gaap_CostOfSalesMember_9b8eb43b-f729-4d23-b9f7-9341ef38576b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResearchAndDevelopmentExpenseMember_07f24b69-fa74-4207-94e9-41d364306bb6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ResearchAndDevelopmentExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_aea2172e-11f0-42ce-9b4f-6cfe2c55051e" xlink:to="loc_us-gaap_ResearchAndDevelopmentExpenseMember_07f24b69-fa74-4207-94e9-41d364306bb6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SellingAndMarketingExpenseMember_6099f6eb-396a-4f5f-a0f9-c845274c1db2" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SellingAndMarketingExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_aea2172e-11f0-42ce-9b4f-6cfe2c55051e" xlink:to="loc_us-gaap_SellingAndMarketingExpenseMember_6099f6eb-396a-4f5f-a0f9-c845274c1db2" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GeneralAndAdministrativeExpenseMember_325df27b-51bb-49a2-b4c8-30148fd2bde8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_GeneralAndAdministrativeExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_aea2172e-11f0-42ce-9b4f-6cfe2c55051e" xlink:to="loc_us-gaap_GeneralAndAdministrativeExpenseMember_325df27b-51bb-49a2-b4c8-30148fd2bde8" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_TitleOfIndividualAxis_de06ddba-3c96-4f57-9f69-854eea43bfb4" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_TitleOfIndividualAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfEmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsTable_b3258a4a-c457-4262-9451-d53bd0e6f720" xlink:to="loc_srt_TitleOfIndividualAxis_de06ddba-3c96-4f57-9f69-854eea43bfb4" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_de06ddba-3c96-4f57-9f69-854eea43bfb4_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_TitleOfIndividualWithRelationshipToEntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_TitleOfIndividualAxis_de06ddba-3c96-4f57-9f69-854eea43bfb4" xlink:to="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_de06ddba-3c96-4f57-9f69-854eea43bfb4_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_ae07287b-d8e5-47bf-b499-683bab4ea9ff" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_TitleOfIndividualWithRelationshipToEntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_TitleOfIndividualAxis_de06ddba-3c96-4f57-9f69-854eea43bfb4" xlink:to="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_ae07287b-d8e5-47bf-b499-683bab4ea9ff" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_FormerExecutiveRetiredMember_7a684754-3903-45ef-b632-42afff21ad00" xlink:href="wk-20241231.xsd#wk_FormerExecutiveRetiredMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_ae07287b-d8e5-47bf-b499-683bab4ea9ff" xlink:to="loc_wk_FormerExecutiveRetiredMember_7a684754-3903-45ef-b632-42afff21ad00" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllocatedShareBasedCompensationExpense_42ef8039-c5d0-417f-a627-0742af0c39af" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AllocatedShareBasedCompensationExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems_812d5762-6cf0-43b5-85fc-71d43218c7b3" xlink:to="loc_us-gaap_AllocatedShareBasedCompensationExpense_42ef8039-c5d0-417f-a627-0742af0c39af" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.workiva.com/role/StockBasedCompensationStockOptionsDetails" xlink:type="simple" xlink:href="wk-20241231.xsd#StockBasedCompensationStockOptionsDetails"/>
  <link:definitionLink xlink:role="http://www.workiva.com/role/StockBasedCompensationStockOptionsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_d0eed2a5-43a1-4fd8-8dcc-ba0b0668b237" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_8d88b12b-8e4f-4aec-9a7e-a6ac9a8d420c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_d0eed2a5-43a1-4fd8-8dcc-ba0b0668b237" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_8d88b12b-8e4f-4aec-9a7e-a6ac9a8d420c" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_c9dce392-f2b7-4203-9d00-f2c8b33f157b" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_8d88b12b-8e4f-4aec-9a7e-a6ac9a8d420c" xlink:to="loc_srt_RangeAxis_c9dce392-f2b7-4203-9d00-f2c8b33f157b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_c9dce392-f2b7-4203-9d00-f2c8b33f157b_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_c9dce392-f2b7-4203-9d00-f2c8b33f157b" xlink:to="loc_srt_RangeMember_c9dce392-f2b7-4203-9d00-f2c8b33f157b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_46769c37-e69f-41e6-9f0b-2130a78e5f5a" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_c9dce392-f2b7-4203-9d00-f2c8b33f157b" xlink:to="loc_srt_RangeMember_46769c37-e69f-41e6-9f0b-2130a78e5f5a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_f2c2f5a5-e71a-4418-a9c9-a8c6f268688a" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_46769c37-e69f-41e6-9f0b-2130a78e5f5a" xlink:to="loc_srt_MinimumMember_f2c2f5a5-e71a-4418-a9c9-a8c6f268688a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_7c970342-2891-483f-8fc8-a1db3b552630" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_46769c37-e69f-41e6-9f0b-2130a78e5f5a" xlink:to="loc_srt_MaximumMember_7c970342-2891-483f-8fc8-a1db3b552630" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_5cb3d659-125c-4cb8-a3ed-a013749628ab" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_8d88b12b-8e4f-4aec-9a7e-a6ac9a8d420c" xlink:to="loc_us-gaap_StatementClassOfStockAxis_5cb3d659-125c-4cb8-a3ed-a013749628ab" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_5cb3d659-125c-4cb8-a3ed-a013749628ab_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_5cb3d659-125c-4cb8-a3ed-a013749628ab" xlink:to="loc_us-gaap_ClassOfStockDomain_5cb3d659-125c-4cb8-a3ed-a013749628ab_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_6b8fbc58-7582-4ed2-bbc4-95640669b610" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_5cb3d659-125c-4cb8-a3ed-a013749628ab" xlink:to="loc_us-gaap_ClassOfStockDomain_6b8fbc58-7582-4ed2-bbc4-95640669b610" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonClassAMember_3ea597ee-fefe-41ab-83ca-98ace13216dd" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonClassAMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_6b8fbc58-7582-4ed2-bbc4-95640669b610" xlink:to="loc_us-gaap_CommonClassAMember_3ea597ee-fefe-41ab-83ca-98ace13216dd" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_b33ac75d-505e-4ae2-8a65-9999d72fb548" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_8d88b12b-8e4f-4aec-9a7e-a6ac9a8d420c" xlink:to="loc_us-gaap_AwardTypeAxis_b33ac75d-505e-4ae2-8a65-9999d72fb548" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_b33ac75d-505e-4ae2-8a65-9999d72fb548_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_b33ac75d-505e-4ae2-8a65-9999d72fb548" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_b33ac75d-505e-4ae2-8a65-9999d72fb548_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_f9cbd6cc-8ddb-4879-a839-9af10040f064" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_b33ac75d-505e-4ae2-8a65-9999d72fb548" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_f9cbd6cc-8ddb-4879-a839-9af10040f064" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockOptionMember_0faa7316-ad1e-43b8-b6a9-d8cb63ab9228" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EmployeeStockOptionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_f9cbd6cc-8ddb-4879-a839-9af10040f064" xlink:to="loc_us-gaap_EmployeeStockOptionMember_0faa7316-ad1e-43b8-b6a9-d8cb63ab9228" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingRollForward_d5651dcc-f6ef-44cc-86a1-6f5350251cd4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_d0eed2a5-43a1-4fd8-8dcc-ba0b0668b237" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingRollForward_d5651dcc-f6ef-44cc-86a1-6f5350251cd4" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingNumber_b1c6a830-ef65-4506-8029-89c96a90c27e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingRollForward_d5651dcc-f6ef-44cc-86a1-6f5350251cd4" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingNumber_b1c6a830-ef65-4506-8029-89c96a90c27e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodGross_cfaa202b-6fa0-4cab-963a-320770a2fd2e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingRollForward_d5651dcc-f6ef-44cc-86a1-6f5350251cd4" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodGross_cfaa202b-6fa0-4cab-963a-320770a2fd2e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsForfeituresInPeriod_8ca5b952-5984-4402-896a-4a62ad54d643" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsForfeituresInPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingRollForward_d5651dcc-f6ef-44cc-86a1-6f5350251cd4" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsForfeituresInPeriod_8ca5b952-5984-4402-896a-4a62ad54d643" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExpirationsInPeriod_2ed694d7-a9f0-44e2-8fc7-caf56e79992c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExpirationsInPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingRollForward_d5651dcc-f6ef-44cc-86a1-6f5350251cd4" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExpirationsInPeriod_2ed694d7-a9f0-44e2-8fc7-caf56e79992c" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised_e80b41ff-cd51-4efc-bc04-b000ccde766a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingRollForward_d5651dcc-f6ef-44cc-86a1-6f5350251cd4" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised_e80b41ff-cd51-4efc-bc04-b000ccde766a" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingNumber_5222337b-5c62-4b1d-bf26-9c5e46656cb7" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingNumber"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableNumber_5b292b55-c4bb-4bec-bdec-0b10ee4b08c6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_d0eed2a5-43a1-4fd8-8dcc-ba0b0668b237" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableNumber_5b292b55-c4bb-4bec-bdec-0b10ee4b08c6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePriceRollforward_5551d800-4c13-4df7-8993-c30e01f3568c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePriceRollforward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_d0eed2a5-43a1-4fd8-8dcc-ba0b0668b237" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePriceRollforward_5551d800-4c13-4df7-8993-c30e01f3568c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePrice_735781af-c392-49dd-9e94-d15eb50e4c20" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePrice"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePriceRollforward_5551d800-4c13-4df7-8993-c30e01f3568c" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePrice_735781af-c392-49dd-9e94-d15eb50e4c20" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsGrantsInPeriodWeightedAverageExercisePrice_973b0046-0e56-4995-8048-730387e8ea88" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsGrantsInPeriodWeightedAverageExercisePrice"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePriceRollforward_5551d800-4c13-4df7-8993-c30e01f3568c" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsGrantsInPeriodWeightedAverageExercisePrice_973b0046-0e56-4995-8048-730387e8ea88" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsForfeituresInPeriodWeightedAverageExercisePrice_b607ad8e-3606-42c8-a859-eca30708a3e3" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsForfeituresInPeriodWeightedAverageExercisePrice"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePriceRollforward_5551d800-4c13-4df7-8993-c30e01f3568c" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsForfeituresInPeriodWeightedAverageExercisePrice_b607ad8e-3606-42c8-a859-eca30708a3e3" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsExpirationsInPeriodWeightedAverageExercisePrice_ca038014-892f-4b55-8cae-1dd8eb9e6837" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsExpirationsInPeriodWeightedAverageExercisePrice"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePriceRollforward_5551d800-4c13-4df7-8993-c30e01f3568c" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsExpirationsInPeriodWeightedAverageExercisePrice_ca038014-892f-4b55-8cae-1dd8eb9e6837" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsExercisesInPeriodWeightedAverageExercisePrice_79394412-1b39-483b-9b22-b326906caaa7" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsExercisesInPeriodWeightedAverageExercisePrice"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePriceRollforward_5551d800-4c13-4df7-8993-c30e01f3568c" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsExercisesInPeriodWeightedAverageExercisePrice_79394412-1b39-483b-9b22-b326906caaa7" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePrice_3dc1ad82-bf25-47e2-916d-cc88cec27981" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePrice"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableWeightedAverageExercisePrice_2b3d6e38-45fe-473b-9018-9cd9fe2c2112" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableWeightedAverageExercisePrice"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_d0eed2a5-43a1-4fd8-8dcc-ba0b0668b237" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableWeightedAverageExercisePrice_2b3d6e38-45fe-473b-9018-9cd9fe2c2112" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsOutstandingWeightedAverageRemainingContractualTerm2_09fdcf63-3cb4-4789-8595-cd6fd081403b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsOutstandingWeightedAverageRemainingContractualTerm2"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_d0eed2a5-43a1-4fd8-8dcc-ba0b0668b237" xlink:to="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsOutstandingWeightedAverageRemainingContractualTerm2_09fdcf63-3cb4-4789-8595-cd6fd081403b" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsExercisableWeightedAverageRemainingContractualTerm1_f358ea52-5252-407c-b912-339cfdd58ed4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsExercisableWeightedAverageRemainingContractualTerm1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_d0eed2a5-43a1-4fd8-8dcc-ba0b0668b237" xlink:to="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsExercisableWeightedAverageRemainingContractualTerm1_f358ea52-5252-407c-b912-339cfdd58ed4" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingIntrinsicValue_4f3ce2ba-b067-4c3a-ab18-badfd5e68e63" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingIntrinsicValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_d0eed2a5-43a1-4fd8-8dcc-ba0b0668b237" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingIntrinsicValue_4f3ce2ba-b067-4c3a-ab18-badfd5e68e63" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsExercisableIntrinsicValue1_d07bdfa7-4e46-4de2-a1a1-b3e62f7f890e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsExercisableIntrinsicValue1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_d0eed2a5-43a1-4fd8-8dcc-ba0b0668b237" xlink:to="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsExercisableIntrinsicValue1_d07bdfa7-4e46-4de2-a1a1-b3e62f7f890e" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsAdditionalDisclosuresAbstract_5353508a-076b-4fae-ab36-d657b7315350" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsAdditionalDisclosuresAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_d0eed2a5-43a1-4fd8-8dcc-ba0b0668b237" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsAdditionalDisclosuresAbstract_5353508a-076b-4fae-ab36-d657b7315350" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardAwardVestingPeriod1_36bc61c1-e709-4de4-857e-9f0bad891613" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardAwardVestingPeriod1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsAdditionalDisclosuresAbstract_5353508a-076b-4fae-ab36-d657b7315350" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardAwardVestingPeriod1_36bc61c1-e709-4de4-857e-9f0bad891613" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardExpirationPeriod_be45f067-e802-4f94-8f74-7207ebd66519" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardExpirationPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsAdditionalDisclosuresAbstract_5353508a-076b-4fae-ab36-d657b7315350" xlink:to="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardExpirationPeriod_be45f067-e802-4f94-8f74-7207ebd66519" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisesInPeriodTotalIntrinsicValue_4f3c87df-329c-4a6f-b35b-a932f2029db4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisesInPeriodTotalIntrinsicValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsAdditionalDisclosuresAbstract_5353508a-076b-4fae-ab36-d657b7315350" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisesInPeriodTotalIntrinsicValue_4f3c87df-329c-4a6f-b35b-a932f2029db4" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedStockOptions_91228a30-912c-407d-bfc9-3a17dcaabf35" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedStockOptions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsAdditionalDisclosuresAbstract_5353508a-076b-4fae-ab36-d657b7315350" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedStockOptions_91228a30-912c-407d-bfc9-3a17dcaabf35" xlink:type="arc" order="4"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.workiva.com/role/StockBasedCompensationRestrictedStockUnitsandPerformanceRestrictedStockUnitsDetails" xlink:type="simple" xlink:href="wk-20241231.xsd#StockBasedCompensationRestrictedStockUnitsandPerformanceRestrictedStockUnitsDetails"/>
  <link:definitionLink xlink:role="http://www.workiva.com/role/StockBasedCompensationRestrictedStockUnitsandPerformanceRestrictedStockUnitsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_6b97de88-1080-4454-b371-c560b271dbb4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_a82f95fd-e12c-45b8-969d-f40578cc7de0" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_6b97de88-1080-4454-b371-c560b271dbb4" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_a82f95fd-e12c-45b8-969d-f40578cc7de0" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VestingAxis_970d0a4f-90ef-4e27-a5c5-229daa3acfee" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_VestingAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_a82f95fd-e12c-45b8-969d-f40578cc7de0" xlink:to="loc_us-gaap_VestingAxis_970d0a4f-90ef-4e27-a5c5-229daa3acfee" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VestingDomain_970d0a4f-90ef-4e27-a5c5-229daa3acfee_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_VestingDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_VestingAxis_970d0a4f-90ef-4e27-a5c5-229daa3acfee" xlink:to="loc_us-gaap_VestingDomain_970d0a4f-90ef-4e27-a5c5-229daa3acfee_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VestingDomain_3a1fe94e-a4fd-46bf-8795-ed8ac9a2d44a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_VestingDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_VestingAxis_970d0a4f-90ef-4e27-a5c5-229daa3acfee" xlink:to="loc_us-gaap_VestingDomain_3a1fe94e-a4fd-46bf-8795-ed8ac9a2d44a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationAwardTrancheOneMember_cf1bb218-5d20-453a-99a9-36e444211a03" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationAwardTrancheOneMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_VestingDomain_3a1fe94e-a4fd-46bf-8795-ed8ac9a2d44a" xlink:to="loc_us-gaap_ShareBasedCompensationAwardTrancheOneMember_cf1bb218-5d20-453a-99a9-36e444211a03" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_3968e8e6-5d43-418c-8e0a-b6f4f7684557" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_a82f95fd-e12c-45b8-969d-f40578cc7de0" xlink:to="loc_srt_RangeAxis_3968e8e6-5d43-418c-8e0a-b6f4f7684557" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_3968e8e6-5d43-418c-8e0a-b6f4f7684557_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_3968e8e6-5d43-418c-8e0a-b6f4f7684557" xlink:to="loc_srt_RangeMember_3968e8e6-5d43-418c-8e0a-b6f4f7684557_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_0a9d2ec0-d391-45e9-96aa-10eed7925d0a" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_3968e8e6-5d43-418c-8e0a-b6f4f7684557" xlink:to="loc_srt_RangeMember_0a9d2ec0-d391-45e9-96aa-10eed7925d0a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_6b7e6bb4-48bd-40ff-ad41-e9a1efe11762" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_0a9d2ec0-d391-45e9-96aa-10eed7925d0a" xlink:to="loc_srt_MinimumMember_6b7e6bb4-48bd-40ff-ad41-e9a1efe11762" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_6d9f2992-807c-4e68-bcd8-2ae6f0b14068" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_0a9d2ec0-d391-45e9-96aa-10eed7925d0a" xlink:to="loc_srt_MaximumMember_6d9f2992-807c-4e68-bcd8-2ae6f0b14068" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_TitleOfIndividualAxis_293c5d67-a905-448d-a666-8ea8d3d08ab4" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_TitleOfIndividualAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_a82f95fd-e12c-45b8-969d-f40578cc7de0" xlink:to="loc_srt_TitleOfIndividualAxis_293c5d67-a905-448d-a666-8ea8d3d08ab4" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_293c5d67-a905-448d-a666-8ea8d3d08ab4_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_TitleOfIndividualWithRelationshipToEntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_TitleOfIndividualAxis_293c5d67-a905-448d-a666-8ea8d3d08ab4" xlink:to="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_293c5d67-a905-448d-a666-8ea8d3d08ab4_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_e2addb51-9ea0-4365-b6dd-95d2d8e61868" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_TitleOfIndividualWithRelationshipToEntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_TitleOfIndividualAxis_293c5d67-a905-448d-a666-8ea8d3d08ab4" xlink:to="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_e2addb51-9ea0-4365-b6dd-95d2d8e61868" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_DirectorMember_54b51229-4858-4436-848d-445e8e08303c" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_DirectorMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_e2addb51-9ea0-4365-b6dd-95d2d8e61868" xlink:to="loc_srt_DirectorMember_54b51229-4858-4436-848d-445e8e08303c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_1aabb7bb-53d2-4cb8-a361-045317b18685" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_a82f95fd-e12c-45b8-969d-f40578cc7de0" xlink:to="loc_us-gaap_AwardTypeAxis_1aabb7bb-53d2-4cb8-a361-045317b18685" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_1aabb7bb-53d2-4cb8-a361-045317b18685_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_1aabb7bb-53d2-4cb8-a361-045317b18685" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_1aabb7bb-53d2-4cb8-a361-045317b18685_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_2b6d93cb-2e10-482c-b7d8-3f038686ef69" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_1aabb7bb-53d2-4cb8-a361-045317b18685" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_2b6d93cb-2e10-482c-b7d8-3f038686ef69" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_RestrictedStockUnitsAndPerformanceSharesMember_73db66a8-9c34-4b1f-9155-73a4801d754c" xlink:href="wk-20241231.xsd#wk_RestrictedStockUnitsAndPerformanceSharesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_2b6d93cb-2e10-482c-b7d8-3f038686ef69" xlink:to="loc_wk_RestrictedStockUnitsAndPerformanceSharesMember_73db66a8-9c34-4b1f-9155-73a4801d754c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockUnitsRSUMember_073aec01-c0be-41d7-88e4-30ed102b0d27" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RestrictedStockUnitsRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_wk_RestrictedStockUnitsAndPerformanceSharesMember_73db66a8-9c34-4b1f-9155-73a4801d754c" xlink:to="loc_us-gaap_RestrictedStockUnitsRSUMember_073aec01-c0be-41d7-88e4-30ed102b0d27" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PerformanceSharesMember_ce6f342f-a7cd-43c1-9672-0aa36146893d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PerformanceSharesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_wk_RestrictedStockUnitsAndPerformanceSharesMember_73db66a8-9c34-4b1f-9155-73a4801d754c" xlink:to="loc_us-gaap_PerformanceSharesMember_ce6f342f-a7cd-43c1-9672-0aa36146893d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardAwardVestingPeriod1_fbc1a4a2-bb9a-442c-84e3-cdf7cd68d1d3" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardAwardVestingPeriod1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_6b97de88-1080-4454-b371-c560b271dbb4" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardAwardVestingPeriod1_fbc1a4a2-bb9a-442c-84e3-cdf7cd68d1d3" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodTotalFairValue_8be9146b-4756-42b5-97d7-d53a879373bb" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodTotalFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_6b97de88-1080-4454-b371-c560b271dbb4" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodTotalFairValue_8be9146b-4756-42b5-97d7-d53a879373bb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedRollForward_af15820c-dc34-47fd-88bf-82d7b6159fd4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_6b97de88-1080-4454-b371-c560b271dbb4" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedRollForward_af15820c-dc34-47fd-88bf-82d7b6159fd4" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber_4a1cc31b-22ef-41db-ad76-fdebdcaa6c25" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedRollForward_af15820c-dc34-47fd-88bf-82d7b6159fd4" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber_4a1cc31b-22ef-41db-ad76-fdebdcaa6c25" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriod_c7a2e93f-26d9-4605-9d50-2faa994fc266" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedRollForward_af15820c-dc34-47fd-88bf-82d7b6159fd4" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriod_c7a2e93f-26d9-4605-9d50-2faa994fc266" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeitedInPeriod_dfaae520-5a9b-4216-8b9f-0a85d76a53ba" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeitedInPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedRollForward_af15820c-dc34-47fd-88bf-82d7b6159fd4" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeitedInPeriod_dfaae520-5a9b-4216-8b9f-0a85d76a53ba" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriod_e2469c6e-b63d-45a0-adbd-5d05f3877a1f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedRollForward_af15820c-dc34-47fd-88bf-82d7b6159fd4" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriod_e2469c6e-b63d-45a0-adbd-5d05f3877a1f" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber_a882ec3b-8558-4dc4-b7fb-2ec5cf6817cf" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward_8500973a-f967-4598-938f-f1286aa93924" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_6b97de88-1080-4454-b371-c560b271dbb4" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward_8500973a-f967-4598-938f-f1286aa93924" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue_9e3672c0-7475-4732-9f5f-2252b95c2f69" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward_8500973a-f967-4598-938f-f1286aa93924" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue_9e3672c0-7475-4732-9f5f-2252b95c2f69" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriodWeightedAverageGrantDateFairValue_6d19bb39-7d74-4641-92ad-830f51c22e33" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriodWeightedAverageGrantDateFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward_8500973a-f967-4598-938f-f1286aa93924" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriodWeightedAverageGrantDateFairValue_6d19bb39-7d74-4641-92ad-830f51c22e33" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeituresWeightedAverageGrantDateFairValue_ef42ab5f-2e7c-4d1a-a029-0e6fd57519a9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeituresWeightedAverageGrantDateFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward_8500973a-f967-4598-938f-f1286aa93924" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeituresWeightedAverageGrantDateFairValue_ef42ab5f-2e7c-4d1a-a029-0e6fd57519a9" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodWeightedAverageGrantDateFairValue_80e441f6-8228-47ec-8971-0246d4fefd59" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodWeightedAverageGrantDateFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward_8500973a-f967-4598-938f-f1286aa93924" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodWeightedAverageGrantDateFairValue_80e441f6-8228-47ec-8971-0246d4fefd59" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue_0ab7cb77-3748-4c10-bd5e-845749225b1d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedAndDeferred_2192407d-f286-4a37-b106-f646be95989b" xlink:href="wk-20241231.xsd#wk_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedAndDeferred"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_6b97de88-1080-4454-b371-c560b271dbb4" xlink:to="loc_wk_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedAndDeferred_2192407d-f286-4a37-b106-f646be95989b" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsReleasedFromDeferral_7a48d706-4e2f-4cf9-ad6b-3b44a14cca69" xlink:href="wk-20241231.xsd#wk_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsReleasedFromDeferral"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_6b97de88-1080-4454-b371-c560b271dbb4" xlink:to="loc_wk_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsReleasedFromDeferral_7a48d706-4e2f-4cf9-ad6b-3b44a14cca69" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedAndOutstanding_c1b5a4a3-3f4d-4744-b4b2-96d637b5bd2d" xlink:href="wk-20241231.xsd#wk_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedAndOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_6b97de88-1080-4454-b371-c560b271dbb4" xlink:to="loc_wk_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedAndOutstanding_c1b5a4a3-3f4d-4744-b4b2-96d637b5bd2d" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedShareBasedAwardsOtherThanOptions_7453b5f9-bc74-402a-906e-ff681983d7e4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedShareBasedAwardsOtherThanOptions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_6b97de88-1080-4454-b371-c560b271dbb4" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedShareBasedAwardsOtherThanOptions_7453b5f9-bc74-402a-906e-ff681983d7e4" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1_bb26913b-72d3-4daf-a1a8-63ef5d01321f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_6b97de88-1080-4454-b371-c560b271dbb4" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1_bb26913b-72d3-4daf-a1a8-63ef5d01321f" xlink:type="arc" order="8"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.workiva.com/role/StockBasedCompensationEmployeeStockPurchasePlanAssumptionsDetails" xlink:type="simple" xlink:href="wk-20241231.xsd#StockBasedCompensationEmployeeStockPurchasePlanAssumptionsDetails"/>
  <link:definitionLink xlink:role="http://www.workiva.com/role/StockBasedCompensationEmployeeStockPurchasePlanAssumptionsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_a48fed0d-54e3-4f7d-aeef-dca477876482" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_26413b1f-3698-4c07-94d9-21cb3961b2fc" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_a48fed0d-54e3-4f7d-aeef-dca477876482" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_26413b1f-3698-4c07-94d9-21cb3961b2fc" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_dcdf2fcc-c1f8-4f5f-8340-7f1158cdea98" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_26413b1f-3698-4c07-94d9-21cb3961b2fc" xlink:to="loc_us-gaap_AwardTypeAxis_dcdf2fcc-c1f8-4f5f-8340-7f1158cdea98" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_dcdf2fcc-c1f8-4f5f-8340-7f1158cdea98_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_dcdf2fcc-c1f8-4f5f-8340-7f1158cdea98" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_dcdf2fcc-c1f8-4f5f-8340-7f1158cdea98_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_377474b5-ad77-45ab-9412-be9c27a04a7b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_dcdf2fcc-c1f8-4f5f-8340-7f1158cdea98" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_377474b5-ad77-45ab-9412-be9c27a04a7b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockMember_dc97cec6-7e50-4bad-b7c1-eb3653cd03c5" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EmployeeStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_377474b5-ad77-45ab-9412-be9c27a04a7b" xlink:to="loc_us-gaap_EmployeeStockMember_dc97cec6-7e50-4bad-b7c1-eb3653cd03c5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsAndMethodologyAbstract_d71af78f-36aa-4a26-97f0-355e78b6bbdf" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsAndMethodologyAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_a48fed0d-54e3-4f7d-aeef-dca477876482" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsAndMethodologyAbstract_d71af78f-36aa-4a26-97f0-355e78b6bbdf" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardFairValueAssumptionsExpectedTerm1_2067ae2e-c604-46aa-baf5-8361b891655b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardFairValueAssumptionsExpectedTerm1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsAndMethodologyAbstract_d71af78f-36aa-4a26-97f0-355e78b6bbdf" xlink:to="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardFairValueAssumptionsExpectedTerm1_2067ae2e-c604-46aa-baf5-8361b891655b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsRiskFreeInterestRateMinimum_3ef73e87-f9b7-48a6-9d18-60208d289031" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsRiskFreeInterestRateMinimum"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsAndMethodologyAbstract_d71af78f-36aa-4a26-97f0-355e78b6bbdf" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsRiskFreeInterestRateMinimum_3ef73e87-f9b7-48a6-9d18-60208d289031" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsRiskFreeInterestRateMaximum_045c8fa9-926a-4c10-8d48-fc452a978b81" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsRiskFreeInterestRateMaximum"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsAndMethodologyAbstract_d71af78f-36aa-4a26-97f0-355e78b6bbdf" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsRiskFreeInterestRateMaximum_045c8fa9-926a-4c10-8d48-fc452a978b81" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedVolatilityRateMinimum_fdc54453-3e8b-4bd9-a55d-e97803759d77" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedVolatilityRateMinimum"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsAndMethodologyAbstract_d71af78f-36aa-4a26-97f0-355e78b6bbdf" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedVolatilityRateMinimum_fdc54453-3e8b-4bd9-a55d-e97803759d77" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedVolatilityRateMaximum_49a64067-c262-417b-a5bb-1af5b8f64621" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedVolatilityRateMaximum"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsAndMethodologyAbstract_d71af78f-36aa-4a26-97f0-355e78b6bbdf" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedVolatilityRateMaximum_49a64067-c262-417b-a5bb-1af5b8f64621" xlink:type="arc" order="5"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.workiva.com/role/StockBasedCompensationEmployeeStockPurchaseDetails" xlink:type="simple" xlink:href="wk-20241231.xsd#StockBasedCompensationEmployeeStockPurchaseDetails"/>
  <link:definitionLink xlink:role="http://www.workiva.com/role/StockBasedCompensationEmployeeStockPurchaseDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_6ed15d35-c0ea-43fe-aeb9-f0c3f4c435c5" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_6b019351-0118-41a2-8b70-be693c4509de" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_6ed15d35-c0ea-43fe-aeb9-f0c3f4c435c5" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_6b019351-0118-41a2-8b70-be693c4509de" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_5dc090e2-471a-4f0c-9c91-04ae2702e397" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_6b019351-0118-41a2-8b70-be693c4509de" xlink:to="loc_us-gaap_AwardTypeAxis_5dc090e2-471a-4f0c-9c91-04ae2702e397" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_5dc090e2-471a-4f0c-9c91-04ae2702e397_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_5dc090e2-471a-4f0c-9c91-04ae2702e397" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_5dc090e2-471a-4f0c-9c91-04ae2702e397_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_6938067a-bd2e-4ad0-b52d-3befe09b8de2" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_5dc090e2-471a-4f0c-9c91-04ae2702e397" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_6938067a-bd2e-4ad0-b52d-3befe09b8de2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockMember_fa194aab-7877-49c9-872b-c0d609658229" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EmployeeStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_6938067a-bd2e-4ad0-b52d-3befe09b8de2" xlink:to="loc_us-gaap_EmployeeStockMember_fa194aab-7877-49c9-872b-c0d609658229" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardSharesIssuedInPeriod_cb29559b-748d-4ee6-b6fc-d1a2ad47136b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardSharesIssuedInPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_6ed15d35-c0ea-43fe-aeb9-f0c3f4c435c5" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardSharesIssuedInPeriod_cb29559b-748d-4ee6-b6fc-d1a2ad47136b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_ShareBasedCompensationArrangementByShareBasedPaymentAwardSharesIssuedInPeriodWeightedAveragePricePerShare_173cce97-980f-47ee-8c86-039588969feb" xlink:href="wk-20241231.xsd#wk_ShareBasedCompensationArrangementByShareBasedPaymentAwardSharesIssuedInPeriodWeightedAveragePricePerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_6ed15d35-c0ea-43fe-aeb9-f0c3f4c435c5" xlink:to="loc_wk_ShareBasedCompensationArrangementByShareBasedPaymentAwardSharesIssuedInPeriodWeightedAveragePricePerShare_173cce97-980f-47ee-8c86-039588969feb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromIssuanceOfSharesUnderIncentiveAndShareBasedCompensationPlans_82c8a236-9143-4e90-b67a-70da57976f41" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProceedsFromIssuanceOfSharesUnderIncentiveAndShareBasedCompensationPlans"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_6ed15d35-c0ea-43fe-aeb9-f0c3f4c435c5" xlink:to="loc_us-gaap_ProceedsFromIssuanceOfSharesUnderIncentiveAndShareBasedCompensationPlans_82c8a236-9143-4e90-b67a-70da57976f41" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedShareBasedAwardsOtherThanOptions_7fde6441-5b21-4a41-af5a-af245fb62393" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedShareBasedAwardsOtherThanOptions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_6ed15d35-c0ea-43fe-aeb9-f0c3f4c435c5" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedShareBasedAwardsOtherThanOptions_7fde6441-5b21-4a41-af5a-af245fb62393" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1_c42cc033-f4b0-4596-a5d2-25dc431009a3" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_6ed15d35-c0ea-43fe-aeb9-f0c3f4c435c5" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1_c42cc033-f4b0-4596-a5d2-25dc431009a3" xlink:type="arc" order="4"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.workiva.com/role/AccumulatedOtherComprehensiveLossIncomeDetails" xlink:type="simple" xlink:href="wk-20241231.xsd#AccumulatedOtherComprehensiveLossIncomeDetails"/>
  <link:definitionLink xlink:role="http://www.workiva.com/role/AccumulatedOtherComprehensiveLossIncomeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_85b9f328-5fb0-4be8-afec-437d11b7775c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossTable_e0f6e523-69c1-434a-ba8c-927f1ff3381e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeLossTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_85b9f328-5fb0-4be8-afec-437d11b7775c" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossTable_e0f6e523-69c1-434a-ba8c-927f1ff3381e" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_057f3e89-c800-4e87-b629-dbc28d13385e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossTable_e0f6e523-69c1-434a-ba8c-927f1ff3381e" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_057f3e89-c800-4e87-b629-dbc28d13385e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_057f3e89-c800-4e87-b629-dbc28d13385e_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_057f3e89-c800-4e87-b629-dbc28d13385e" xlink:to="loc_us-gaap_EquityComponentDomain_057f3e89-c800-4e87-b629-dbc28d13385e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_20b612fb-3212-4fd4-8a30-9e06cee30d9b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_057f3e89-c800-4e87-b629-dbc28d13385e" xlink:to="loc_us-gaap_EquityComponentDomain_20b612fb-3212-4fd4-8a30-9e06cee30d9b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_6028cb3a-2e4c-4d86-9cbb-c998be9f4789" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_20b612fb-3212-4fd4-8a30-9e06cee30d9b" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_6028cb3a-2e4c-4d86-9cbb-c998be9f4789" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedTranslationAdjustmentMember_fea25dcb-0116-465a-9db2-3e2335b383f0" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccumulatedTranslationAdjustmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_6028cb3a-2e4c-4d86-9cbb-c998be9f4789" xlink:to="loc_us-gaap_AccumulatedTranslationAdjustmentMember_fea25dcb-0116-465a-9db2-3e2335b383f0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedNetUnrealizedInvestmentGainLossMember_de68cdb6-9b3e-4feb-8355-43a13d62f2a8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccumulatedNetUnrealizedInvestmentGainLossMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_6028cb3a-2e4c-4d86-9cbb-c998be9f4789" xlink:to="loc_us-gaap_AccumulatedNetUnrealizedInvestmentGainLossMember_de68cdb6-9b3e-4feb-8355-43a13d62f2a8" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AOCIAttributableToParentNetOfTaxRollForward_7e8aee02-98d3-4b21-8c0d-70cc27b5ea40" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AOCIAttributableToParentNetOfTaxRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_85b9f328-5fb0-4be8-afec-437d11b7775c" xlink:to="loc_us-gaap_AOCIAttributableToParentNetOfTaxRollForward_7e8aee02-98d3-4b21-8c0d-70cc27b5ea40" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_123782a3-e593-474b-a3cb-2ad042be9cb3" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockholdersEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AOCIAttributableToParentNetOfTaxRollForward_7e8aee02-98d3-4b21-8c0d-70cc27b5ea40" xlink:to="loc_us-gaap_StockholdersEquity_123782a3-e593-474b-a3cb-2ad042be9cb3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_558a62da-4466-4eed-a39a-d6ef610f7de7" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AOCIAttributableToParentNetOfTaxRollForward_7e8aee02-98d3-4b21-8c0d-70cc27b5ea40" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_558a62da-4466-4eed-a39a-d6ef610f7de7" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_568afb3c-8f23-43a2-8a54-c05e4d451065" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockholdersEquity"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.workiva.com/role/AcquisitionsNarrativeDetails" xlink:type="simple" xlink:href="wk-20241231.xsd#AcquisitionsNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.workiva.com/role/AcquisitionsNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionLineItems_58b42d3d-0fde-4680-808e-a340fc66ad24" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessAcquisitionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_58af4c50-7430-4c48-8de8-4940b0d19824" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_58b42d3d-0fde-4680-808e-a340fc66ad24" xlink:to="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_58af4c50-7430-4c48-8de8-4940b0d19824" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAxis_ddf3f756-adb1-49e1-ab48-34d9c4a05176" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessAcquisitionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_58af4c50-7430-4c48-8de8-4940b0d19824" xlink:to="loc_us-gaap_BusinessAcquisitionAxis_ddf3f756-adb1-49e1-ab48-34d9c4a05176" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_ddf3f756-adb1-49e1-ab48-34d9c4a05176_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_ddf3f756-adb1-49e1-ab48-34d9c4a05176" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_ddf3f756-adb1-49e1-ab48-34d9c4a05176_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_7e0bd865-b867-47a3-bee0-2a3c67110fcf" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_ddf3f756-adb1-49e1-ab48-34d9c4a05176" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_7e0bd865-b867-47a3-bee0-2a3c67110fcf" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_Sustain.LifeMember_2d5f7fea-6487-435e-97be-1d1ade37dabd" xlink:href="wk-20241231.xsd#wk_Sustain.LifeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionAcquireeDomain_7e0bd865-b867-47a3-bee0-2a3c67110fcf" xlink:to="loc_wk_Sustain.LifeMember_2d5f7fea-6487-435e-97be-1d1ade37dabd" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_ParsePortApSMember_a44e09fb-be6d-4432-9108-12f426106817" xlink:href="wk-20241231.xsd#wk_ParsePortApSMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionAcquireeDomain_7e0bd865-b867-47a3-bee0-2a3c67110fcf" xlink:to="loc_wk_ParsePortApSMember_a44e09fb-be6d-4432-9108-12f426106817" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquireBusinessesNetOfCashAcquired_d780d30f-000a-432a-8b37-f83c09730c85" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PaymentsToAcquireBusinessesNetOfCashAcquired"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_58b42d3d-0fde-4680-808e-a340fc66ad24" xlink:to="loc_us-gaap_PaymentsToAcquireBusinessesNetOfCashAcquired_d780d30f-000a-432a-8b37-f83c09730c85" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAcquiredFromAcquisition_510ebf6e-5055-48d4-b628-a2ac3462b45c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CashAcquiredFromAcquisition"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_58b42d3d-0fde-4680-808e-a340fc66ad24" xlink:to="loc_us-gaap_CashAcquiredFromAcquisition_510ebf6e-5055-48d4-b628-a2ac3462b45c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionPurchasePriceAllocationGoodwillExpectedTaxDeductibleAmount_c47565a3-484f-443d-94e9-92b34285228a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessAcquisitionPurchasePriceAllocationGoodwillExpectedTaxDeductibleAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_58b42d3d-0fde-4680-808e-a340fc66ad24" xlink:to="loc_us-gaap_BusinessAcquisitionPurchasePriceAllocationGoodwillExpectedTaxDeductibleAmount_c47565a3-484f-443d-94e9-92b34285228a" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationAcquisitionRelatedCosts_539dbb93-4867-469d-9644-ecd3d98cc7cb" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationAcquisitionRelatedCosts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_58b42d3d-0fde-4680-808e-a340fc66ad24" xlink:to="loc_us-gaap_BusinessCombinationAcquisitionRelatedCosts_539dbb93-4867-469d-9644-ecd3d98cc7cb" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.workiva.com/role/AcquisitionsAssetsAcquiredandLiabilitiesAssumedDetails" xlink:type="simple" xlink:href="wk-20241231.xsd#AcquisitionsAssetsAcquiredandLiabilitiesAssumedDetails"/>
  <link:definitionLink xlink:role="http://www.workiva.com/role/AcquisitionsAssetsAcquiredandLiabilitiesAssumedDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionLineItems_84ba0f22-b7de-425d-bdd7-0e21f48ad5ef" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessAcquisitionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_97de02ea-bbfb-4a75-9ef4-4be954b87c56" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_84ba0f22-b7de-425d-bdd7-0e21f48ad5ef" xlink:to="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_97de02ea-bbfb-4a75-9ef4-4be954b87c56" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAxis_3a2f063b-79f6-4253-b8d9-d3f263f9e3e4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessAcquisitionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_97de02ea-bbfb-4a75-9ef4-4be954b87c56" xlink:to="loc_us-gaap_BusinessAcquisitionAxis_3a2f063b-79f6-4253-b8d9-d3f263f9e3e4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_3a2f063b-79f6-4253-b8d9-d3f263f9e3e4_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_3a2f063b-79f6-4253-b8d9-d3f263f9e3e4" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_3a2f063b-79f6-4253-b8d9-d3f263f9e3e4_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_c193c7c2-cad0-4ac8-a647-d40b828be3b4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_3a2f063b-79f6-4253-b8d9-d3f263f9e3e4" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_c193c7c2-cad0-4ac8-a647-d40b828be3b4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_Sustain.LifeMember_1dfc88a2-f03d-4c73-a811-3027fb7a6403" xlink:href="wk-20241231.xsd#wk_Sustain.LifeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionAcquireeDomain_c193c7c2-cad0-4ac8-a647-d40b828be3b4" xlink:to="loc_wk_Sustain.LifeMember_1dfc88a2-f03d-4c73-a811-3027fb7a6403" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_ParsePortApSMember_286e38b2-98aa-4ca4-954f-83f1358f5595" xlink:href="wk-20241231.xsd#wk_ParsePortApSMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionAcquireeDomain_c193c7c2-cad0-4ac8-a647-d40b828be3b4" xlink:to="loc_wk_ParsePortApSMember_286e38b2-98aa-4ca4-954f-83f1358f5595" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquireBusinessesGross_4d3f2fc9-73cb-4b6b-98a9-ea9640954b23" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PaymentsToAcquireBusinessesGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_84ba0f22-b7de-425d-bdd7-0e21f48ad5ef" xlink:to="loc_us-gaap_PaymentsToAcquireBusinessesGross_4d3f2fc9-73cb-4b6b-98a9-ea9640954b23" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationConsiderationTransferred1_850688ba-6718-4876-aca0-116e6a86c7e3" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationConsiderationTransferred1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_84ba0f22-b7de-425d-bdd7-0e21f48ad5ef" xlink:to="loc_us-gaap_BusinessCombinationConsiderationTransferred1_850688ba-6718-4876-aca0-116e6a86c7e3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCashAndEquivalents_4ef6fc77-1117-450a-adc1-6c7da8e1bd27" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCashAndEquivalents"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_84ba0f22-b7de-425d-bdd7-0e21f48ad5ef" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCashAndEquivalents_4ef6fc77-1117-450a-adc1-6c7da8e1bd27" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentAssetsAccountsReceivables_8a685863-c271-4eef-9be6-752fb6a5ffb7" xlink:href="wk-20241231.xsd#wk_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentAssetsAccountsReceivables"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_84ba0f22-b7de-425d-bdd7-0e21f48ad5ef" xlink:to="loc_wk_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentAssetsAccountsReceivables_8a685863-c271-4eef-9be6-752fb6a5ffb7" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentAssetsOtherReceivables_35e8a473-a0cb-4f5f-87b7-8c1793b6cf24" xlink:href="wk-20241231.xsd#wk_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentAssetsOtherReceivables"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_84ba0f22-b7de-425d-bdd7-0e21f48ad5ef" xlink:to="loc_wk_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentAssetsOtherReceivables_35e8a473-a0cb-4f5f-87b7-8c1793b6cf24" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentAssetsReceivables_9d8321de-68e1-49a6-bb2a-537042ceed81" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentAssetsReceivables"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_84ba0f22-b7de-425d-bdd7-0e21f48ad5ef" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentAssetsReceivables_9d8321de-68e1-49a6-bb2a-537042ceed81" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentAssetsPrepaidExpenseAndOtherAssets_7c2e3b3f-d8bd-45eb-a11b-dfb9d842f46c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentAssetsPrepaidExpenseAndOtherAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_84ba0f22-b7de-425d-bdd7-0e21f48ad5ef" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentAssetsPrepaidExpenseAndOtherAssets_7c2e3b3f-d8bd-45eb-a11b-dfb9d842f46c" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedIntangibleAssetsOtherThanGoodwill_6a1e0ce8-15db-4888-9ba2-220ffb108995" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedIntangibleAssetsOtherThanGoodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_84ba0f22-b7de-425d-bdd7-0e21f48ad5ef" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedIntangibleAssetsOtherThanGoodwill_6a1e0ce8-15db-4888-9ba2-220ffb108995" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedIntangibles_0edcb55a-def6-4bbf-9d5e-7f6d7c7515fd" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedIntangibles"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_84ba0f22-b7de-425d-bdd7-0e21f48ad5ef" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedIntangibles_0edcb55a-def6-4bbf-9d5e-7f6d7c7515fd" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Goodwill_f738986c-713e-48bc-8618-83277c333391" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_Goodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_84ba0f22-b7de-425d-bdd7-0e21f48ad5ef" xlink:to="loc_us-gaap_Goodwill_f738986c-713e-48bc-8618-83277c333391" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedOtherNoncurrentAssets_873730fe-f164-479d-a53f-a5fd53b67a19" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedOtherNoncurrentAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_84ba0f22-b7de-425d-bdd7-0e21f48ad5ef" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedOtherNoncurrentAssets_873730fe-f164-479d-a53f-a5fd53b67a19" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentLiabilitiesAccountsPayable_5191866a-6092-43b7-933c-38367a3e1719" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentLiabilitiesAccountsPayable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_84ba0f22-b7de-425d-bdd7-0e21f48ad5ef" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentLiabilitiesAccountsPayable_5191866a-6092-43b7-933c-38367a3e1719" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentLiabilitiesAccruedLiabilities_67bb3df9-71a7-4bc4-b1f7-62dd8b57988a" xlink:href="wk-20241231.xsd#wk_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentLiabilitiesAccruedLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_84ba0f22-b7de-425d-bdd7-0e21f48ad5ef" xlink:to="loc_wk_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentLiabilitiesAccruedLiabilities_67bb3df9-71a7-4bc4-b1f7-62dd8b57988a" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedContractWithCustomerLiability_f2e5ed8c-1152-4cea-a17d-bc5a824ead3c" xlink:href="wk-20241231.xsd#wk_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedContractWithCustomerLiability"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_84ba0f22-b7de-425d-bdd7-0e21f48ad5ef" xlink:to="loc_wk_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedContractWithCustomerLiability_f2e5ed8c-1152-4cea-a17d-bc5a824ead3c" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedNoncurrentLiabilitiesOther_584ef1f6-2642-4684-87c7-d10ce9f1453f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedNoncurrentLiabilitiesOther"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_84ba0f22-b7de-425d-bdd7-0e21f48ad5ef" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedNoncurrentLiabilitiesOther_584ef1f6-2642-4684-87c7-d10ce9f1453f" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredGoodwillAndLiabilitiesAssumedNet_79707b90-8c27-4d53-90b5-3d375cdd0f5c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredGoodwillAndLiabilitiesAssumedNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_84ba0f22-b7de-425d-bdd7-0e21f48ad5ef" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredGoodwillAndLiabilitiesAssumedNet_79707b90-8c27-4d53-90b5-3d375cdd0f5c" xlink:type="arc" order="15"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.workiva.com/role/GoodwillandIntangibleAssetsIntangibleAssetComponentsDetails" xlink:type="simple" xlink:href="wk-20241231.xsd#GoodwillandIntangibleAssetsIntangibleAssetComponentsDetails"/>
  <link:definitionLink xlink:role="http://www.workiva.com/role/GoodwillandIntangibleAssetsIntangibleAssetComponentsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_63402b86-4395-4b1e-88b2-5ef7d6a5aae3" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FiniteLivedIntangibleAssetsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_58201d82-9a23-469d-aff0-0c1104c0f986" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_63402b86-4395-4b1e-88b2-5ef7d6a5aae3" xlink:to="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_58201d82-9a23-469d-aff0-0c1104c0f986" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_b14bef4f-f6d8-40c5-a5eb-5c86a1bfd433" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_58201d82-9a23-469d-aff0-0c1104c0f986" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_b14bef4f-f6d8-40c5-a5eb-5c86a1bfd433" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_b14bef4f-f6d8-40c5-a5eb-5c86a1bfd433_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_b14bef4f-f6d8-40c5-a5eb-5c86a1bfd433" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_b14bef4f-f6d8-40c5-a5eb-5c86a1bfd433_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_2690cde5-65fb-48aa-a4f9-c60290e81cfc" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_b14bef4f-f6d8-40c5-a5eb-5c86a1bfd433" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_2690cde5-65fb-48aa-a4f9-c60290e81cfc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TechnologyBasedIntangibleAssetsMember_e3f69a66-9613-4926-9dd7-895f6e50cd2e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_TechnologyBasedIntangibleAssetsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_2690cde5-65fb-48aa-a4f9-c60290e81cfc" xlink:to="loc_us-gaap_TechnologyBasedIntangibleAssetsMember_e3f69a66-9613-4926-9dd7-895f6e50cd2e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CustomerRelationshipsMember_c8ebefa7-1347-4e43-a1a2-e4926d6f591f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CustomerRelationshipsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_2690cde5-65fb-48aa-a4f9-c60290e81cfc" xlink:to="loc_us-gaap_CustomerRelationshipsMember_c8ebefa7-1347-4e43-a1a2-e4926d6f591f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TradeNamesMember_720a0bb5-f4ef-4c8d-830b-b5da8890d825" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_TradeNamesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_2690cde5-65fb-48aa-a4f9-c60290e81cfc" xlink:to="loc_us-gaap_TradeNamesMember_720a0bb5-f4ef-4c8d-830b-b5da8890d825" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PatentsMember_ac516f48-cd69-4f1b-9374-e6eee87cc5bf" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PatentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_2690cde5-65fb-48aa-a4f9-c60290e81cfc" xlink:to="loc_us-gaap_PatentsMember_ac516f48-cd69-4f1b-9374-e6eee87cc5bf" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetUsefulLife_8122996c-86e2-4a0b-bdeb-9c57d05a7529" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FiniteLivedIntangibleAssetUsefulLife"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_63402b86-4395-4b1e-88b2-5ef7d6a5aae3" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetUsefulLife_8122996c-86e2-4a0b-bdeb-9c57d05a7529" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsGross_59065b5e-cb86-454f-9fe3-b24484f447a8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FiniteLivedIntangibleAssetsGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_63402b86-4395-4b1e-88b2-5ef7d6a5aae3" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsGross_59065b5e-cb86-454f-9fe3-b24484f447a8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_28ec99ff-3ee0-4c45-8039-b4364c23969f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_63402b86-4395-4b1e-88b2-5ef7d6a5aae3" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_28ec99ff-3ee0-4c45-8039-b4364c23969f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsNet_8f4b5297-e429-43a3-9272-758c96098d90" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FiniteLivedIntangibleAssetsNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_63402b86-4395-4b1e-88b2-5ef7d6a5aae3" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsNet_8f4b5297-e429-43a3-9272-758c96098d90" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.workiva.com/role/GeographicInformationRevenueandLonglivedAssetsDetails" xlink:type="simple" xlink:href="wk-20241231.xsd#GeographicInformationRevenueandLonglivedAssetsDetails"/>
  <link:definitionLink xlink:role="http://www.workiva.com/role/GeographicInformationRevenueandLonglivedAssetsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenuesFromExternalCustomersAndLongLivedAssetsLineItems_2768b3ed-d6d5-425d-bba2-b8eb18accf11" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RevenuesFromExternalCustomersAndLongLivedAssetsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRevenuesFromExternalCustomersAndLongLivedAssetsTable_834409bf-96b6-43bd-a312-3ffae9ed4487" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfRevenuesFromExternalCustomersAndLongLivedAssetsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RevenuesFromExternalCustomersAndLongLivedAssetsLineItems_2768b3ed-d6d5-425d-bba2-b8eb18accf11" xlink:to="loc_us-gaap_ScheduleOfRevenuesFromExternalCustomersAndLongLivedAssetsTable_834409bf-96b6-43bd-a312-3ffae9ed4487" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductOrServiceAxis_205cd568-c962-49a1-8b24-1009c4ca52f5" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ProductOrServiceAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRevenuesFromExternalCustomersAndLongLivedAssetsTable_834409bf-96b6-43bd-a312-3ffae9ed4487" xlink:to="loc_srt_ProductOrServiceAxis_205cd568-c962-49a1-8b24-1009c4ca52f5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_205cd568-c962-49a1-8b24-1009c4ca52f5_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ProductOrServiceAxis_205cd568-c962-49a1-8b24-1009c4ca52f5" xlink:to="loc_srt_ProductsAndServicesDomain_205cd568-c962-49a1-8b24-1009c4ca52f5_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_2ce1478a-1aa4-4b5f-bb81-d3c4659ce413" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ProductOrServiceAxis_205cd568-c962-49a1-8b24-1009c4ca52f5" xlink:to="loc_srt_ProductsAndServicesDomain_2ce1478a-1aa4-4b5f-bb81-d3c4659ce413" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LicenseAndServiceMember_abcbe31b-e2ca-4567-8265-48cfc47360a5" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LicenseAndServiceMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_2ce1478a-1aa4-4b5f-bb81-d3c4659ce413" xlink:to="loc_us-gaap_LicenseAndServiceMember_abcbe31b-e2ca-4567-8265-48cfc47360a5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_XBRLProfessionalServicesMember_0831150f-686b-406c-8c98-ce44061710a8" xlink:href="wk-20241231.xsd#wk_XBRLProfessionalServicesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_2ce1478a-1aa4-4b5f-bb81-d3c4659ce413" xlink:to="loc_wk_XBRLProfessionalServicesMember_0831150f-686b-406c-8c98-ce44061710a8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementGeographicalAxis_95ba7a0d-4666-4bd8-9b44-b30d7d276e13" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_StatementGeographicalAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRevenuesFromExternalCustomersAndLongLivedAssetsTable_834409bf-96b6-43bd-a312-3ffae9ed4487" xlink:to="loc_srt_StatementGeographicalAxis_95ba7a0d-4666-4bd8-9b44-b30d7d276e13" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_95ba7a0d-4666-4bd8-9b44-b30d7d276e13_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementGeographicalAxis_95ba7a0d-4666-4bd8-9b44-b30d7d276e13" xlink:to="loc_srt_SegmentGeographicalDomain_95ba7a0d-4666-4bd8-9b44-b30d7d276e13_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_0574a40e-b3e9-4d9b-b39c-a53a5ce1ee94" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementGeographicalAxis_95ba7a0d-4666-4bd8-9b44-b30d7d276e13" xlink:to="loc_srt_SegmentGeographicalDomain_0574a40e-b3e9-4d9b-b39c-a53a5ce1ee94" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_AmericasMember_abe45767-e4e0-4ce6-8fed-2f89d8173b31" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_AmericasMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_0574a40e-b3e9-4d9b-b39c-a53a5ce1ee94" xlink:to="loc_srt_AmericasMember_abe45767-e4e0-4ce6-8fed-2f89d8173b31" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_OtherCountriesExcludingAmericasMember_57778a5e-1bcc-4f11-bc01-81e33dd72e41" xlink:href="wk-20241231.xsd#wk_OtherCountriesExcludingAmericasMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_0574a40e-b3e9-4d9b-b39c-a53a5ce1ee94" xlink:to="loc_wk_OtherCountriesExcludingAmericasMember_57778a5e-1bcc-4f11-bc01-81e33dd72e41" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_US_625b1871-06a9-4bcf-bf5e-4b8c968f26d4" xlink:href="https://xbrl.sec.gov/country/2024/country-2024.xsd#country_US"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_0574a40e-b3e9-4d9b-b39c-a53a5ce1ee94" xlink:to="loc_country_US_625b1871-06a9-4bcf-bf5e-4b8c968f26d4" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_NL_c0affa94-6b29-4a5b-a086-04692f132cb2" xlink:href="https://xbrl.sec.gov/country/2024/country-2024.xsd#country_NL"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_0574a40e-b3e9-4d9b-b39c-a53a5ce1ee94" xlink:to="loc_country_NL_c0affa94-6b29-4a5b-a086-04692f132cb2" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_GB_30fe59b0-2930-4993-a427-53fffdb1614d" xlink:href="https://xbrl.sec.gov/country/2024/country-2024.xsd#country_GB"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_0574a40e-b3e9-4d9b-b39c-a53a5ce1ee94" xlink:to="loc_country_GB_30fe59b0-2930-4993-a427-53fffdb1614d" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_OtherCountriesExcludingUnitedStatesNetherlandsAndUnitedKingdomMember_9ac85361-2456-46c9-84de-182956d6a9b6" xlink:href="wk-20241231.xsd#wk_OtherCountriesExcludingUnitedStatesNetherlandsAndUnitedKingdomMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_0574a40e-b3e9-4d9b-b39c-a53a5ce1ee94" xlink:to="loc_wk_OtherCountriesExcludingUnitedStatesNetherlandsAndUnitedKingdomMember_9ac85361-2456-46c9-84de-182956d6a9b6" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_c48e1909-6fa4-4bdb-86c7-3a723cf849ed" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenuesFromExternalCustomersAndLongLivedAssetsLineItems_2768b3ed-d6d5-425d-bba2-b8eb18accf11" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_c48e1909-6fa4-4bdb-86c7-3a723cf849ed" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NoncurrentAssets_60896b19-d946-4c50-8ce1-5d5e6304b11f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NoncurrentAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenuesFromExternalCustomersAndLongLivedAssetsLineItems_2768b3ed-d6d5-425d-bba2-b8eb18accf11" xlink:to="loc_us-gaap_NoncurrentAssets_60896b19-d946-4c50-8ce1-5d5e6304b11f" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.workiva.com/role/GeographicInformationNarrativeDetails" xlink:type="simple" xlink:href="wk-20241231.xsd#GeographicInformationNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.workiva.com/role/GeographicInformationNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskLineItems_d045108c-a64a-4d70-92d7-6992d2093c88" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ConcentrationRiskLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskTable_f22cae91-229b-4e39-bb78-cf38f06f8287" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ConcentrationRiskTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ConcentrationRiskLineItems_d045108c-a64a-4d70-92d7-6992d2093c88" xlink:to="loc_us-gaap_ConcentrationRiskTable_f22cae91-229b-4e39-bb78-cf38f06f8287" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_e3b2241f-426b-4239-9a25-68e27bd285ea" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ConcentrationRiskByBenchmarkAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ConcentrationRiskTable_f22cae91-229b-4e39-bb78-cf38f06f8287" xlink:to="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_e3b2241f-426b-4239-9a25-68e27bd285ea" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskBenchmarkDomain_e3b2241f-426b-4239-9a25-68e27bd285ea_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ConcentrationRiskBenchmarkDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_e3b2241f-426b-4239-9a25-68e27bd285ea" xlink:to="loc_us-gaap_ConcentrationRiskBenchmarkDomain_e3b2241f-426b-4239-9a25-68e27bd285ea_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskBenchmarkDomain_051e2304-904e-4de3-9cd7-5c87fa87f36e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ConcentrationRiskBenchmarkDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_e3b2241f-426b-4239-9a25-68e27bd285ea" xlink:to="loc_us-gaap_ConcentrationRiskBenchmarkDomain_051e2304-904e-4de3-9cd7-5c87fa87f36e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_RevenueFromContractWithCustomerAmericasRevenueMember_eb22d708-a809-4915-9f16-90c4077426b5" xlink:href="wk-20241231.xsd#wk_RevenueFromContractWithCustomerAmericasRevenueMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskBenchmarkDomain_051e2304-904e-4de3-9cd7-5c87fa87f36e" xlink:to="loc_wk_RevenueFromContractWithCustomerAmericasRevenueMember_eb22d708-a809-4915-9f16-90c4077426b5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskByTypeAxis_5a9b25c7-4f1a-4795-81d0-7d6aa9e1829a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ConcentrationRiskByTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ConcentrationRiskTable_f22cae91-229b-4e39-bb78-cf38f06f8287" xlink:to="loc_us-gaap_ConcentrationRiskByTypeAxis_5a9b25c7-4f1a-4795-81d0-7d6aa9e1829a" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskTypeDomain_5a9b25c7-4f1a-4795-81d0-7d6aa9e1829a_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ConcentrationRiskTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ConcentrationRiskByTypeAxis_5a9b25c7-4f1a-4795-81d0-7d6aa9e1829a" xlink:to="loc_us-gaap_ConcentrationRiskTypeDomain_5a9b25c7-4f1a-4795-81d0-7d6aa9e1829a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskTypeDomain_31ba4787-6400-419b-a572-50d326b36ee2" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ConcentrationRiskTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ConcentrationRiskByTypeAxis_5a9b25c7-4f1a-4795-81d0-7d6aa9e1829a" xlink:to="loc_us-gaap_ConcentrationRiskTypeDomain_31ba4787-6400-419b-a572-50d326b36ee2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GeographicConcentrationRiskMember_afa401dd-1ea1-4b77-9a42-3eecf3d0243c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_GeographicConcentrationRiskMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskTypeDomain_31ba4787-6400-419b-a572-50d326b36ee2" xlink:to="loc_us-gaap_GeographicConcentrationRiskMember_afa401dd-1ea1-4b77-9a42-3eecf3d0243c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementGeographicalAxis_bc2587dc-f093-4c90-93f2-c9ee3a3b02be" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_StatementGeographicalAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ConcentrationRiskTable_f22cae91-229b-4e39-bb78-cf38f06f8287" xlink:to="loc_srt_StatementGeographicalAxis_bc2587dc-f093-4c90-93f2-c9ee3a3b02be" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_bc2587dc-f093-4c90-93f2-c9ee3a3b02be_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementGeographicalAxis_bc2587dc-f093-4c90-93f2-c9ee3a3b02be" xlink:to="loc_srt_SegmentGeographicalDomain_bc2587dc-f093-4c90-93f2-c9ee3a3b02be_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_0ea00c6b-fcdd-436c-849f-9586308aee51" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementGeographicalAxis_bc2587dc-f093-4c90-93f2-c9ee3a3b02be" xlink:to="loc_srt_SegmentGeographicalDomain_0ea00c6b-fcdd-436c-849f-9586308aee51" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_US_7f8967f2-eedb-40d6-9c61-4c56c435fcf2" xlink:href="https://xbrl.sec.gov/country/2024/country-2024.xsd#country_US"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_0ea00c6b-fcdd-436c-849f-9586308aee51" xlink:to="loc_country_US_7f8967f2-eedb-40d6-9c61-4c56c435fcf2" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskPercentage1_3fb53276-24a8-4800-907b-7375e6814beb" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ConcentrationRiskPercentage1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskLineItems_d045108c-a64a-4d70-92d7-6992d2093c88" xlink:to="loc_us-gaap_ConcentrationRiskPercentage1_3fb53276-24a8-4800-907b-7375e6814beb" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.workiva.com/role/RevenueRecognitionDisaggregationofRevenueDetails" xlink:type="simple" xlink:href="wk-20241231.xsd#RevenueRecognitionDisaggregationofRevenueDetails"/>
  <link:definitionLink xlink:role="http://www.workiva.com/role/RevenueRecognitionDisaggregationofRevenueDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisaggregationOfRevenueLineItems_0a6b5f5e-8fab-49e2-8420-ae8f56046ece" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DisaggregationOfRevenueLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisaggregationOfRevenueTable_f7c889b8-906c-451b-8e5b-624d5c8744fb" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DisaggregationOfRevenueTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_0a6b5f5e-8fab-49e2-8420-ae8f56046ece" xlink:to="loc_us-gaap_DisaggregationOfRevenueTable_f7c889b8-906c-451b-8e5b-624d5c8744fb" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductOrServiceAxis_e5a5f5c3-6e06-445b-ac63-c1bb682e1927" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ProductOrServiceAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DisaggregationOfRevenueTable_f7c889b8-906c-451b-8e5b-624d5c8744fb" xlink:to="loc_srt_ProductOrServiceAxis_e5a5f5c3-6e06-445b-ac63-c1bb682e1927" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_e5a5f5c3-6e06-445b-ac63-c1bb682e1927_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ProductOrServiceAxis_e5a5f5c3-6e06-445b-ac63-c1bb682e1927" xlink:to="loc_srt_ProductsAndServicesDomain_e5a5f5c3-6e06-445b-ac63-c1bb682e1927_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_cc52b714-4cd2-477d-857b-470138b1247c" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ProductOrServiceAxis_e5a5f5c3-6e06-445b-ac63-c1bb682e1927" xlink:to="loc_srt_ProductsAndServicesDomain_cc52b714-4cd2-477d-857b-470138b1247c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LicenseAndServiceMember_af38a14d-a96f-4b0e-9da8-ecb0c166ad1f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LicenseAndServiceMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_cc52b714-4cd2-477d-857b-470138b1247c" xlink:to="loc_us-gaap_LicenseAndServiceMember_af38a14d-a96f-4b0e-9da8-ecb0c166ad1f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_XBRLProfessionalServicesMember_ac241d11-7df0-454f-838e-fbb46520ef98" xlink:href="wk-20241231.xsd#wk_XBRLProfessionalServicesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_cc52b714-4cd2-477d-857b-470138b1247c" xlink:to="loc_wk_XBRLProfessionalServicesMember_ac241d11-7df0-454f-838e-fbb46520ef98" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_OtherServicesMember_8b4ec602-980e-4312-ab9e-ea8136b97d2f" xlink:href="wk-20241231.xsd#wk_OtherServicesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_cc52b714-4cd2-477d-857b-470138b1247c" xlink:to="loc_wk_OtherServicesMember_8b4ec602-980e-4312-ab9e-ea8136b97d2f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_42c13477-9de2-4f18-8f29-626bf7ac1736" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_0a6b5f5e-8fab-49e2-8420-ae8f56046ece" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_42c13477-9de2-4f18-8f29-626bf7ac1736" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.workiva.com/role/RevenueRecognitionDeferredRevenueandTransactionPriceAllocatedtotheRemainingPerformanceObligationsDetails" xlink:type="simple" xlink:href="wk-20241231.xsd#RevenueRecognitionDeferredRevenueandTransactionPriceAllocatedtotheRemainingPerformanceObligationsDetails"/>
  <link:definitionLink xlink:role="http://www.workiva.com/role/RevenueRecognitionDeferredRevenueandTransactionPriceAllocatedtotheRemainingPerformanceObligationsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems_9ef9a8d7-bb5b-4a86-a24b-4681d758ca14" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionTable_3a6aa1eb-9701-4734-9399-edb68f3d0000" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems_9ef9a8d7-bb5b-4a86-a24b-4681d758ca14" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionTable_3a6aa1eb-9701-4734-9399-edb68f3d0000" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionStartDateAxis_e3cddc19-e3b1-4c7b-8fa0-8304ef5cc30f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionStartDateAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionTable_3a6aa1eb-9701-4734-9399-edb68f3d0000" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionStartDateAxis_e3cddc19-e3b1-4c7b-8fa0-8304ef5cc30f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligation_13ec497f-73c5-4eca-aab7-6b8f59f16016" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RevenueRemainingPerformanceObligation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems_9ef9a8d7-bb5b-4a86-a24b-4681d758ca14" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligation_13ec497f-73c5-4eca-aab7-6b8f59f16016" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionPeriod1_8590829f-631f-4ec0-87db-9ec0045f5a19" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionPeriod1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems_9ef9a8d7-bb5b-4a86-a24b-4681d758ca14" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionPeriod1_8590829f-631f-4ec0-87db-9ec0045f5a19" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.workiva.com/role/RevenueRecognitionDeferredRevenueandTransactionPriceAllocatedtotheRemainingPerformanceObligationsDetails_1" xlink:type="simple" xlink:href="wk-20241231.xsd#RevenueRecognitionDeferredRevenueandTransactionPriceAllocatedtotheRemainingPerformanceObligationsDetails_1"/>
  <link:definitionLink xlink:role="http://www.workiva.com/role/RevenueRecognitionDeferredRevenueandTransactionPriceAllocatedtotheRemainingPerformanceObligationsDetails_1" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems_9ef9a8d7-bb5b-4a86-a24b-4681d758ca14" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionTable_3a6aa1eb-9701-4734-9399-edb68f3d0000" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems_9ef9a8d7-bb5b-4a86-a24b-4681d758ca14" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionTable_3a6aa1eb-9701-4734-9399-edb68f3d0000" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligation_13ec497f-73c5-4eca-aab7-6b8f59f16016" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RevenueRemainingPerformanceObligation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems_9ef9a8d7-bb5b-4a86-a24b-4681d758ca14" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligation_13ec497f-73c5-4eca-aab7-6b8f59f16016" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionPeriod1_8590829f-631f-4ec0-87db-9ec0045f5a19" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionPeriod1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems_9ef9a8d7-bb5b-4a86-a24b-4681d758ca14" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionPeriod1_8590829f-631f-4ec0-87db-9ec0045f5a19" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.workiva.com/role/EmployeeBenefitPlansDetails" xlink:type="simple" xlink:href="wk-20241231.xsd#EmployeeBenefitPlansDetails"/>
  <link:definitionLink xlink:role="http://www.workiva.com/role/EmployeeBenefitPlansDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_c3960b7e-6019-470e-98d2-11e352c77be3" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DefinedBenefitPlanDisclosureLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_fb1ca08d-094c-4df5-a4bb-3cafe5357660" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_c3960b7e-6019-470e-98d2-11e352c77be3" xlink:to="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_fb1ca08d-094c-4df5-a4bb-3cafe5357660" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanSponsorLocationAxis_225f1a42-4202-44d8-b9e4-3509d95fe811" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RetirementPlanSponsorLocationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_fb1ca08d-094c-4df5-a4bb-3cafe5357660" xlink:to="loc_us-gaap_RetirementPlanSponsorLocationAxis_225f1a42-4202-44d8-b9e4-3509d95fe811" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanSponsorLocationDomain_225f1a42-4202-44d8-b9e4-3509d95fe811_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RetirementPlanSponsorLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RetirementPlanSponsorLocationAxis_225f1a42-4202-44d8-b9e4-3509d95fe811" xlink:to="loc_us-gaap_RetirementPlanSponsorLocationDomain_225f1a42-4202-44d8-b9e4-3509d95fe811_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanSponsorLocationDomain_8e178213-8a9d-454c-9004-b62e21fa84cb" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RetirementPlanSponsorLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RetirementPlanSponsorLocationAxis_225f1a42-4202-44d8-b9e4-3509d95fe811" xlink:to="loc_us-gaap_RetirementPlanSponsorLocationDomain_8e178213-8a9d-454c-9004-b62e21fa84cb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DomesticPlanMember_da751a40-1ea9-4a0c-9ba9-d66fd7069fe7" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DomesticPlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RetirementPlanSponsorLocationDomain_8e178213-8a9d-454c-9004-b62e21fa84cb" xlink:to="loc_us-gaap_DomesticPlanMember_da751a40-1ea9-4a0c-9ba9-d66fd7069fe7" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_US_d8d724fe-e135-44d9-a3b2-d7dfbe38449a" xlink:href="https://xbrl.sec.gov/country/2024/country-2024.xsd#country_US"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DomesticPlanMember_da751a40-1ea9-4a0c-9ba9-d66fd7069fe7" xlink:to="loc_country_US_d8d724fe-e135-44d9-a3b2-d7dfbe38449a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ForeignPlanMember_86e5c0b7-219a-46b7-a866-c2ce6c55295e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ForeignPlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RetirementPlanSponsorLocationDomain_8e178213-8a9d-454c-9004-b62e21fa84cb" xlink:to="loc_us-gaap_ForeignPlanMember_86e5c0b7-219a-46b7-a866-c2ce6c55295e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedContributionPlanCostRecognized_189dd13c-bc45-4289-8c79-7d59b6890a87" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DefinedContributionPlanCostRecognized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_c3960b7e-6019-470e-98d2-11e352c77be3" xlink:to="loc_us-gaap_DefinedContributionPlanCostRecognized_189dd13c-bc45-4289-8c79-7d59b6890a87" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.workiva.com/role/IncomeTaxesNarrativeDetails" xlink:type="simple" xlink:href="wk-20241231.xsd#IncomeTaxesNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.workiva.com/role/IncomeTaxesNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLossCarryforwardsLineItems_05561762-74ec-41b3-b539-33549ff5b44c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingLossCarryforwardsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLossCarryforwardsTable_0cddc1a9-e431-4c4e-9e7d-94fe3b56f873" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingLossCarryforwardsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_OperatingLossCarryforwardsLineItems_05561762-74ec-41b3-b539-33549ff5b44c" xlink:to="loc_us-gaap_OperatingLossCarryforwardsTable_0cddc1a9-e431-4c4e-9e7d-94fe3b56f873" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxAuthorityAxis_7d5ecce4-8a70-44bf-82f9-6a25616c2cc4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncomeTaxAuthorityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_OperatingLossCarryforwardsTable_0cddc1a9-e431-4c4e-9e7d-94fe3b56f873" xlink:to="loc_us-gaap_IncomeTaxAuthorityAxis_7d5ecce4-8a70-44bf-82f9-6a25616c2cc4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxAuthorityDomain_7d5ecce4-8a70-44bf-82f9-6a25616c2cc4_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncomeTaxAuthorityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_IncomeTaxAuthorityAxis_7d5ecce4-8a70-44bf-82f9-6a25616c2cc4" xlink:to="loc_us-gaap_IncomeTaxAuthorityDomain_7d5ecce4-8a70-44bf-82f9-6a25616c2cc4_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxAuthorityDomain_c81eba7b-284f-40a9-8633-1b93b317c829" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncomeTaxAuthorityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_IncomeTaxAuthorityAxis_7d5ecce4-8a70-44bf-82f9-6a25616c2cc4" xlink:to="loc_us-gaap_IncomeTaxAuthorityDomain_c81eba7b-284f-40a9-8633-1b93b317c829" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DomesticCountryMember_9798b51b-e6e2-44cd-9a58-a95276b86c44" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DomesticCountryMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeTaxAuthorityDomain_c81eba7b-284f-40a9-8633-1b93b317c829" xlink:to="loc_us-gaap_DomesticCountryMember_9798b51b-e6e2-44cd-9a58-a95276b86c44" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StateAndLocalJurisdictionMember_49724445-cfdf-4379-9e6f-8a3d0922df41" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StateAndLocalJurisdictionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeTaxAuthorityDomain_c81eba7b-284f-40a9-8633-1b93b317c829" xlink:to="loc_us-gaap_StateAndLocalJurisdictionMember_49724445-cfdf-4379-9e6f-8a3d0922df41" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ForeignCountryMember_e4ed6fe9-38cd-415b-a303-3df50d49b945" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ForeignCountryMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeTaxAuthorityDomain_c81eba7b-284f-40a9-8633-1b93b317c829" xlink:to="loc_us-gaap_ForeignCountryMember_e4ed6fe9-38cd-415b-a303-3df50d49b945" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLossCarryforwards_d3c56d45-2207-4871-929a-c89268644618" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingLossCarryforwards"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingLossCarryforwardsLineItems_05561762-74ec-41b3-b539-33549ff5b44c" xlink:to="loc_us-gaap_OperatingLossCarryforwards_d3c56d45-2207-4871-929a-c89268644618" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TaxCreditCarryforwardLineItems_f20b46b9-25e2-4542-ab84-e7ebb6b6f8d5" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_TaxCreditCarryforwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TaxCreditCarryforwardTable_49bd399a-6bbc-4c9f-8a18-31764699b11a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_TaxCreditCarryforwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_TaxCreditCarryforwardLineItems_f20b46b9-25e2-4542-ab84-e7ebb6b6f8d5" xlink:to="loc_us-gaap_TaxCreditCarryforwardTable_49bd399a-6bbc-4c9f-8a18-31764699b11a" xlink:type="arc" order="2" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxAuthorityAxis_f1847780-6a90-4609-819e-b0e0f4657e7e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncomeTaxAuthorityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_TaxCreditCarryforwardTable_49bd399a-6bbc-4c9f-8a18-31764699b11a" xlink:to="loc_us-gaap_IncomeTaxAuthorityAxis_f1847780-6a90-4609-819e-b0e0f4657e7e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxAuthorityDomain_f1847780-6a90-4609-819e-b0e0f4657e7e_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncomeTaxAuthorityDomain"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxAuthorityDomain_14c778b5-8a09-4576-9923-8bc9496d8a9d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncomeTaxAuthorityDomain"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DomesticCountryMember_c4f15a08-5c19-40ec-ae4f-41ca91dbc8f4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DomesticCountryMember"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StateAndLocalJurisdictionMember_a71faaa4-d1b5-4a85-b024-170778e68aa6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StateAndLocalJurisdictionMember"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TaxCreditCarryforwardAmount_ae40d034-d32b-43a5-9412-c03f835b4ee0" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_TaxCreditCarryforwardAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TaxCreditCarryforwardLineItems_f20b46b9-25e2-4542-ab84-e7ebb6b6f8d5" xlink:to="loc_us-gaap_TaxCreditCarryforwardAmount_ae40d034-d32b-43a5-9412-c03f835b4ee0" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.workiva.com/role/NetLossPerShareEarningsPerShareBasicandDilutedDetails" xlink:type="simple" xlink:href="wk-20241231.xsd#NetLossPerShareEarningsPerShareBasicandDilutedDetails"/>
  <link:definitionLink xlink:role="http://www.workiva.com/role/NetLossPerShareEarningsPerShareBasicandDilutedDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockLineItems_ce362b18-dbb2-483b-9a4b-263626c09be4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfStockByClassTable_b6f40721-3e21-4906-bf03-fdb0ea877018" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfStockByClassTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ClassOfStockLineItems_ce362b18-dbb2-483b-9a4b-263626c09be4" xlink:to="loc_us-gaap_ScheduleOfStockByClassTable_b6f40721-3e21-4906-bf03-fdb0ea877018" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_e422aba9-34e7-4288-8f21-7b7e0c485026" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfStockByClassTable_b6f40721-3e21-4906-bf03-fdb0ea877018" xlink:to="loc_us-gaap_StatementClassOfStockAxis_e422aba9-34e7-4288-8f21-7b7e0c485026" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_e422aba9-34e7-4288-8f21-7b7e0c485026_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_e422aba9-34e7-4288-8f21-7b7e0c485026" xlink:to="loc_us-gaap_ClassOfStockDomain_e422aba9-34e7-4288-8f21-7b7e0c485026_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_f2215be1-488b-4098-807d-33dc1fbc4a3f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_e422aba9-34e7-4288-8f21-7b7e0c485026" xlink:to="loc_us-gaap_ClassOfStockDomain_f2215be1-488b-4098-807d-33dc1fbc4a3f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonClassAMember_e0abc0c5-d93e-4683-a34d-f9f1e979343a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonClassAMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_f2215be1-488b-4098-807d-33dc1fbc4a3f" xlink:to="loc_us-gaap_CommonClassAMember_e0abc0c5-d93e-4683-a34d-f9f1e979343a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonClassBMember_d2f5ad2a-8a2a-45dd-91e7-37056f8116f1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonClassBMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_f2215be1-488b-4098-807d-33dc1fbc4a3f" xlink:to="loc_us-gaap_CommonClassBMember_d2f5ad2a-8a2a-45dd-91e7-37056f8116f1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLossAvailableToCommonStockholdersDilutedAbstract_b656e125-e0a1-4349-9ca2-cc445cdfdb02" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetIncomeLossAvailableToCommonStockholdersDilutedAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_ce362b18-dbb2-483b-9a4b-263626c09be4" xlink:to="loc_us-gaap_NetIncomeLossAvailableToCommonStockholdersDilutedAbstract_b656e125-e0a1-4349-9ca2-cc445cdfdb02" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLossAvailableToCommonStockholdersBasic_f450abc4-6389-44ee-899a-d63d061903ea" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetIncomeLossAvailableToCommonStockholdersBasic"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetIncomeLossAvailableToCommonStockholdersDilutedAbstract_b656e125-e0a1-4349-9ca2-cc445cdfdb02" xlink:to="loc_us-gaap_NetIncomeLossAvailableToCommonStockholdersBasic_f450abc4-6389-44ee-899a-d63d061903ea" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLossAvailableToCommonStockholdersDiluted_3f8640d6-4b79-42c3-87a3-2eff643be0a9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetIncomeLossAvailableToCommonStockholdersDiluted"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetIncomeLossAvailableToCommonStockholdersDilutedAbstract_b656e125-e0a1-4349-9ca2-cc445cdfdb02" xlink:to="loc_us-gaap_NetIncomeLossAvailableToCommonStockholdersDiluted_3f8640d6-4b79-42c3-87a3-2eff643be0a9" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingDilutedDisclosureItemsAbstract_6e69ca52-70e8-4fa2-ad76-7f58cb5b6086" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_WeightedAverageNumberOfSharesOutstandingDilutedDisclosureItemsAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_ce362b18-dbb2-483b-9a4b-263626c09be4" xlink:to="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingDilutedDisclosureItemsAbstract_6e69ca52-70e8-4fa2-ad76-7f58cb5b6086" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingBasic_e5eb697b-822d-4645-b61c-d131f8cad813" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_WeightedAverageNumberOfSharesOutstandingBasic"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingDilutedDisclosureItemsAbstract_6e69ca52-70e8-4fa2-ad76-7f58cb5b6086" xlink:to="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingBasic_e5eb697b-822d-4645-b61c-d131f8cad813" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding_05dec622-50af-453c-9ce9-8ba0204d41b1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingDilutedDisclosureItemsAbstract_6e69ca52-70e8-4fa2-ad76-7f58cb5b6086" xlink:to="loc_us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding_05dec622-50af-453c-9ce9-8ba0204d41b1" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EarningsPerShareBasic_ec8f9c1b-3c7a-4db2-a8f7-ee42c81761a6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EarningsPerShareBasic"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_ce362b18-dbb2-483b-9a4b-263626c09be4" xlink:to="loc_us-gaap_EarningsPerShareBasic_ec8f9c1b-3c7a-4db2-a8f7-ee42c81761a6" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EarningsPerShareDiluted_470cb477-f6b9-42ea-80ad-5dc998b6db6d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EarningsPerShareDiluted"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_ce362b18-dbb2-483b-9a4b-263626c09be4" xlink:to="loc_us-gaap_EarningsPerShareDiluted_470cb477-f6b9-42ea-80ad-5dc998b6db6d" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.workiva.com/role/NetLossPerShareAntidilutiveSecuritiesExcludedfromComputationofEarningsPerShareDetails" xlink:type="simple" xlink:href="wk-20241231.xsd#NetLossPerShareAntidilutiveSecuritiesExcludedfromComputationofEarningsPerShareDetails"/>
  <link:definitionLink xlink:role="http://www.workiva.com/role/NetLossPerShareAntidilutiveSecuritiesExcludedfromComputationofEarningsPerShareDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_6a1c3e6f-63ec-4415-a18d-560d6989d144" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable_0408f8a4-860a-40b7-a2c4-905c3fc9b764" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_6a1c3e6f-63ec-4415-a18d-560d6989d144" xlink:to="loc_us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable_0408f8a4-860a-40b7-a2c4-905c3fc9b764" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_0c8ee405-9a9d-484d-a042-4b730b6ff3ab" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable_0408f8a4-860a-40b7-a2c4-905c3fc9b764" xlink:to="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_0c8ee405-9a9d-484d-a042-4b730b6ff3ab" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesNameDomain_0c8ee405-9a9d-484d-a042-4b730b6ff3ab_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AntidilutiveSecuritiesNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_0c8ee405-9a9d-484d-a042-4b730b6ff3ab" xlink:to="loc_us-gaap_AntidilutiveSecuritiesNameDomain_0c8ee405-9a9d-484d-a042-4b730b6ff3ab_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesNameDomain_d2305708-552f-4205-9416-efc39702b74c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AntidilutiveSecuritiesNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_0c8ee405-9a9d-484d-a042-4b730b6ff3ab" xlink:to="loc_us-gaap_AntidilutiveSecuritiesNameDomain_d2305708-552f-4205-9416-efc39702b74c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockOptionMember_0c101d29-8da6-4bdd-a5d5-b4543f361de7" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EmployeeStockOptionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_d2305708-552f-4205-9416-efc39702b74c" xlink:to="loc_us-gaap_EmployeeStockOptionMember_0c101d29-8da6-4bdd-a5d5-b4543f361de7" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_wk_RestrictedStockUnitsAndPerformanceSharesMember_97f41a31-2f61-45dd-9a19-dc8d32575144" xlink:href="wk-20241231.xsd#wk_RestrictedStockUnitsAndPerformanceSharesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_d2305708-552f-4205-9416-efc39702b74c" xlink:to="loc_wk_RestrictedStockUnitsAndPerformanceSharesMember_97f41a31-2f61-45dd-9a19-dc8d32575144" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockMember_cbd91286-4092-4302-8093-8ce6c80d25db" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EmployeeStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_d2305708-552f-4205-9416-efc39702b74c" xlink:to="loc_us-gaap_EmployeeStockMember_cbd91286-4092-4302-8093-8ce6c80d25db" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConvertibleDebtSecuritiesMember_144d5ecf-b68b-46b3-8778-a1218fb6e6b1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ConvertibleDebtSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_d2305708-552f-4205-9416-efc39702b74c" xlink:to="loc_us-gaap_ConvertibleDebtSecuritiesMember_144d5ecf-b68b-46b3-8778-a1218fb6e6b1" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount_c3e4bd51-a7bb-43b1-8c88-28ba25530acd" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_6a1c3e6f-63ec-4415-a18d-560d6989d144" xlink:to="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount_c3e4bd51-a7bb-43b1-8c88-28ba25530acd" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://fasb.org/us-gaap/role/eedm/ExtensibleEnumerationLists" xlink:type="simple" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-roles-2024.xsd#eedm"/>
  <link:definitionLink xlink:role="http://fasb.org/us-gaap/role/eedm/ExtensibleEnumerationLists" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesCurrentAbstract" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LiabilitiesCurrentAbstract"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccruedLiabilitiesCurrent" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccruedLiabilitiesCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesCurrentAbstract" xlink:to="loc_us-gaap_AccruedLiabilitiesCurrent" xlink:type="arc" order="41"/>
  </link:definitionLink>
</link:linkbase>
